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CRIMIC CR00641

Gene IscU

CG9836

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3R:8823866..8823883	85B2	FBti0200613	80613

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCTTCAGCCATTCGTCCATCTTCTCA	CCTTTTGCAGCATTCGAGCAGAAGTC

Images

Construct Design Sequences

sgRNA sequence	CTGAAAGACAGCGTTTCAGGCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AACCACCATTCACTGTATCC	CAAAGTGATAAGCGCGATAG	CTGTCTTTCAGAACGACAAC	GCTTAGGATGATCTTCTGGG

 

Amplicon 5'

Amplicon 3'

AACCACCATTCACTGTATCCATACCATTTGACTCACCACTCACAGAGCTCTAGCGAACAATAATCGCATACTACGACCTAATGCCAAGTAATATATAGTATATGGTATTGTATTGTATGGTAGAGTACTTATAGTTGTTTTTTTTTTAGTAGTTGTTCAGAGAAACGCTCCACTACATTCACAGATTCCGTTCCGCCTCTCAGTTGGCCACCTTCTTCTGCTGCTTGACCTTGTAGTCAGCCAGGGCCGCCTTGATGGCATCCTCGGCCAGCATGGAGCAATGCAGCTTGACGGGCGGCAGACGCAGCTCCTTGGCGATGTCCGTATTCTTCAGCTTTCCGGCCTCGTCGATGGACTTGCCCTTCACCCACTCGGTGGCCAGGGAGCTGCTGGCAATGGCCGATCCACAGCCGAAGGTCTTGAACTTGGCGTCCACTATCTTGCCGTTCTCGTCCACCTTGATCTGCAGTTTCATCACATCGCCGCAGGCTGGTGCTCCGACCAAGCCGGTGCCCACAGTGACATCCTTCTTGTCCAAAGATCCCACGTTGCGCGGGTTTTCGTAGTGCTCAACGACCTGTGGGTGAAAATAGGTAGGTGTGTTAGTCACCAGGGGGTTGTGCTATCGCGCTTATCACTTTG

CTGTCTTTCAGAACGACAACAAAGTCGTTTCTTATCTATATTATACAGCTTCTAGATATTACGAAAGAACCTTCCGCGCGGCAATTGCGAGTTCTAACTTACATTTTCATGATACAATGCCACCGGGACACTTTGTACGCGCTTCAGCTGCGATCGCAGCAACCGGGAGGAGTTTCGCACCAGGGACATATTTAAATTAAATAAATCAATTTAATGAAGAATTGTTGTGAAATTCAAAGAGATCGAAAAAACACAGGCAACTGCGTGTTAGAAACAGCTGATGGACAGTGTGCCCGTTTTAAGGCAGATGTGAAAGACCAGCTAACGCCGTTGTCGATATGTTTTCGATTTTTTAAAAGATTTCTTATATTATTATTATTTACTCCTTAAAAACAGTTCAGTATAATAAGTTATTTACTATAAGTAATAAATTGTTTTTATCAATAATGAGCCATAAAAAAGCTTGAATAAAGCACCCTCTTGCATTTAAAATCACACTCTGAGTTGGCAGCTTGCCAGGTATTTTGTAAACTCACTCCTATGGTCACTCCCCAATCACAAAGTTTACTATTGCCCATCCACAAATTCTGCCAACTTCCGATATATTTTTACAGCAGCAGACTATGAGTTTCTGAAACTAGTCCCCTAGGTCAATAATATCCACATCCAGTCATGACCTCTCGCAAGCGCAGTGGCAGCGGCTCCACCAAGCGGCCCAAGGAGAAGGTTCGCCCGGAGCACTGGATTTCACGAGCTCCGCCCTCGAAGATTTGTGCTTTGCTTGACTAATTGGAATTTATTGCAGGTGGTCTATATATATGAGCTGTTGTGCCGGGGCGAGGATCCCAGCAGCGAGAGTCCCGAATTTTGGAACGAGTTCTTCCTGCTGCAGCCGAACTTCGAGGCGCTGGAGAATGAGATTGGCAAACTCAACAACGAGCAGCTGCAGCTGGTGAAACCGAACCTGAACACCCTCTTCCAGAGGTGCATCGAAATGCTTGACACGGGTAGACCAGTTTGACCTATTAATATATACCTCCAGCCACCTATAATCCGTGATTTCCCCCAGAAGATCATCCTAAGC

 

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