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CRIMIC CR00642

Gene sens-2

CG31632

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:6828025..6828034	27B4	FBti0200614	80614

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AATCAATTTCCAGCGCGCTGGAGAAG	CATTTTTCATACCACCCTTCCCGCCA

Images

Construct Design Sequences

sgRNA sequence	TACTGGACAACAGTAGTCGGGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ATGGGCGTCACATGAACCTT	GGTCTGGCCGGTCGTAATAT	CTGTTGTCCAGTACTCCGCC	CTGCTGACTAGTGGGCCAAA

 

Amplicon 5'

Amplicon 3'

ATGGGCGTCACATGAACCTTTGGACGCAGAAATCACACCGACTCATCGACTCGATCACAGTCTAATTTTAAAAACAGTTTAGGCATCTAGAAATTGTAGTGACTACACCTAAGTAATGGGTTCTTTAGGTTTTCCTTAAAAACTGCTGGATGAAAAGTGAAAACCGTCGGCTGGCCGGGACAAGCAATTAAATGAAATTTATCAGCGAAAGGAAAAAGGTAAAAAATTGTAGTCTAAAGTTTAGATAGCCTCCTGATTTATTGCCCAGCGATAAGCAGCTGGGAAAAGGGTGCAATATACGGAGCAGGGGTTTGGGGCACAGCCATTCTGTTTTGGTGGGGGGGGAGTAGCAGGCATGTGGGGTGAACACCTCTGATTGATTTGCAGTGTAAAAATAAATAATATAATTGCAATTGGCGACATTCGCGTAGGGGCTTCAAAGCAACGCTGCCGACGGAGCGACAGACCAATACAGCTGATAGCTGATGGCCGATGGCTGATGGCTGATAACTGGTATACACACATTTGGCCAACTGTTGAGTGTTTGGATACGCAATCCCCATCTAATGCCACTCAACGAGTGGAGCTTATGCGGACTAAAGAGCTCTGAGAGGTCTGCTCTCCCACCAACTGATATGCTATCGCTGGGGTGGGCGATCCAAATCCAAATCGGAAACCCAATCGGAATCCAAATCGAAATCCCAATCGGAATCCCAATCGAAATCCAAATGGAAATCCAAATCGAAATCCGAACAACCCAAGTCGAAATCGAAATCTCAACTCAATCCGTAAATCATGCACACACATTCGCCAGGCATGCCAGCGAACAGACCAAATCATGGCCTAATCTATCTAACCGTTCACAGTTTATGGCCCTCTTCTGGCCATATCGCCGTGGCTCTTTATTATTTATACTGTGCTGTTTTTATTTTTCGCCATTTCCACTTTCATATTTGCCGAAAGCGGAATTTGTTTTTTATAATATTACGACCGGCCAGACC

CTGTTGTCCAGTACTCCGCCCTCGATTTAATCGAATCGATACCTGCGAAATATAAAACACTTGGACTCCACTCCGGCGCCCGAACCAACTGATTTTGTGGCCACAAGAGGTGTTATAAATTACGGCCAAAATGAGAATATGTTTGAAAGCCAAATTAGCGGCACATGCCCTGAATTTTAATACAAAATCTATATGTCAAAATCCATAGAAAGTCTCACTGATTAGGAGGTAGTGTAATCAACGTTTTTGGTTTTCGTAGGACTCAAGATTTGCATAAGCCAAATCCTGACGTCCTATAATTACATAAATGATGATGTTTCTTAATTAAAATTATATGCGTAGAATTGCTCGCTTAACATAAAGAACCAAGTATTTCCAAAATGTTTACTAAACAATTTTTATAACAATCCACACCCCCCCCCCCTCAAATTTCGGTAGTTCGCGAGTAATTATAATTGAAATTCCAGAAATTAACACCTCCGGTAGGAGCACCAGCTGCCCTCCGGCAAAGCGTAAACCCAAACCAAATTGTAATGGCTGCAAATCGCCCCAAAACAATAATCATCCGTGTGGGCGGGGTAGACAGTAAACAGGGCGCAGGATAACAATGATTTCAATTACCATGCAGTGTCGAATAATTATTTTACCTTCGTCCTTTTTATGCGAACAAGTGCGTGGAGTGCGTGAATATTGCTCCCGGGCGGATAAAAACAGATGAACGTCAGAGGCCGAAGGGCTGCATGATATTGGGTGTTAGGGGGTTGGGTGTTCCGTGTATCCTGGGTGTCCTGGTTGTTTGGGGTGGACGAGGGCTATGTCTATGTCTAATGTGCACAAATTTATGACTTTGCTGCCGACCGTCGAGGGGTCGTTGATGCCACTTTGCCAGCCTCCAGCAGCCGAAGGACACACCAGGACTCGCCAGGACTCGAGGAGGAATCGCCACCCTGGAGCGACATCGGGCACATGCTTAAGCTCATTATTATCGTTTAAACAAGTCGCTCTACGTGCGCCACCAGGAGCCACTTGTCGTGGCTCTCCTTATCCTGGTTGTAATACTGGTCCTTGGGGTTGCTTTTGGCCCACTAGTCAGCAG

 

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