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CRIMIC CR00646

Gene alpha-Est2

CG2505

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3R:7542950..7542964	84D9	FBti0200616	80616

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	CGACTGCCCATAGTACCATTTGCCAT	ACGTCCTGATCAATAGCCGCTATCTG

Images

Construct Design Sequences

sgRNA sequence	GGACAAAGAACAAGGTCCGAAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CTGCCTCGAGATACAATCGGA	GCCAATAATCACGACATTGACC	GGACCTTGTTCTTTGTCCCCA	AGCCGATCCTGCACTTTCTC

 

Amplicon 5'

Amplicon 3'

CTGCCTCGAGATACAATCGGAATCAGTATCACGGAAACTTTGACCAATCCAACAAACTCGGAAAAAGTTCTTCGAATTTCAAAACCACAAGAACACATATAACATCCGAATTGCACTAAGTGGAATGGATAGCAATTATGACCAAATAACACGTAAATCGAGGCAAAAAGTGCATTGGTGTGCTACCTATCAAAAGAAGTTTAGTTGACATTTTATGCAGCTTTGAGCATTCGGTTTAAGTTTATTTGATCAAATTCAAAGTTATGCCGCAGCTTCAAGAAATTTTATATTCGTGTAATAGCCTTTTGAAAATTCATAATTAATTGAGGCTATTTCAGCTACACTACCGATTAAATAAGCCAGATGGAGTTTTTCGCTATCTGATTTTGTTGAAATGAATCTTCGTCTTCTAACTCTTTTACAAACTTTGTATATATAAAATATCAAATAAATAGGTTGTTTAAATTATTAGCTACTCCATTGAAAATCTCACTAGTTTTTTAATAATTTTTTTTTTTAATTTACTTTTACAAATAATTCAAAATATATATTTCAAATGCATGTATTATTAGCTTACAATGTACAATTTCCTTTATAATTAATGCGCACTATCTTTTACAGTGGAGGACCACGTTATACTTTCACAAATTAAAAATATGTTAAATTATTTTATTAACTTTTATATATTTTATATATATATATAATATATATATATATATATATTTTATGCATGTAATTATTATTATTCGCTGCCAATAAGATATGTCTATCATTTGAAGTATGTATGTTGTCAGGCAGTTACCTATTTTGCTGACCTATGGTGTGCTTGATAATGATCATCCGGCAAAATACTTTTGAGTATTAAAATGTAATCAAATGCTGTCGGCGTCTCTCCTGATTTAAGGTGCTATCACCGAATTTCTCCGATTAGTCGCACGGCATTATTCGCTCGGACCAGAAATACAGCGAAGCCCTCGAAACAAAATATTAGTAGATCCCGAATATAATCCTAGCAAATAGAAAAGAACCAAATCATGGAGGTGCAAGTGGGATGGCCAAAGCTGATCAAGATGGGAGCCCAGTGAGTAATTAGATTACAGGCCAAGAGTGCATGGTCAATGTCGTGATTATTGGC

GGACCTTGTTCTTTGTCCCCAATCAGTCAGTCTGTCTACTATTATCCCCGATCTGATTCAATCTCTCACTCAGGTTGGTTGGCCACAAGGTGCAACAGTACCGGCTCTCCACGGGTCACACAGTGATTTTGGACACCAAGTACGGACAGGTGCGCGGACTCCAAAGGAAGACGGTCTACGACAAGGAGCCGTACTTTGCCTTCGAGGGCATTCCGTACGCCAAGCCCCCCGTGGGTGACCTCCGATTCAGGGCTCCTCAGCCACCGGAACCCTGGCAAGGAGTGCTCAACTGCACTACCAATCGATCCAAGCCCATGCAGAGGAACATGCTCCTGGGAATTGTCGAGGGCAGTGAGGATTGTTTGCATTTGAACGTTTATGTCAAAGCCTTGAAGTCGGAAAAACCGCTGCCCGTAATTGTGTGGATTTACGGAGGTGGTTTCCAAAAGGGTGAGGCCTCCAGGGATATTTACAGTCCGGATTACTTCATGAAGAAGCCAGTGGTATTCGTCGCTATCAACTACAGGTTGGCAGCACTAGGTAAGGAATCAATTTCCATACAAACAAAAGCCGGAAAATTAAAGTATTAAATATGACAGAAATATATGTATGTAAGTCTATTGGACTAATGTACACAGTACTTGTAGTTATTGTATTCGAATGAGAGAAATAGCACATTTTTGATAAGGCGCCTAATCATTCGCATGTAAACTAGGAATAGAAGAAAATGTTCCTACAATATATATACTTTTTATAAGCATTGACAATAACCAATTTTATTAATTTAATAGGTAAGAATAAAAGTATACAAAATTATTCCTATTACGGTATGTTCTAAGAAACATACTAAAGTGGTCAATACACATTTTTTTAACATCTTATAATAAAATATAAAATAAAATATTTATGTAACCTGTCTTCCTTTCAGGATTTCTCAGTCTAAAGGACCCGAAGCTGGATGTTCCTGGCAATGCGGGACTTAAGGACCAAGTAATGGCTCTGCGTTGGATAAGTCAGAACATCGCCCACTTCAATGGCGACCCCAACAACATCACATTGATGGGAGAAAGTGCAGGATCGGCT

 

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