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CRIMIC CR00649

Gene CG4945

CG4945

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:16567177..16567177	53C8	FBti0200617	83182

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	short band	AATACCAGAACCGAGAGCAGTTGGCA	AGTCCGAGCGATAGATGAGCACATTG

Images

Construct Design Sequences

sgRNA sequence	TTTCCCGGGGAAGAGACAATTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AGAGGCAAGCGGGCATTATT	CGATTTCCCGGGGAAGAGAC	GTCTCTTCCCCGGGAAATCG	CACGTCGTAGGTGGTCACAA

 

Amplicon 5'

Amplicon 3'

AGAGGCAAGCGGGCATTATTTTTAATTTAGCTGTTGATATTATGCATAAAGCGAAGAGAGAAAACAAACATCAAAACAGAATAGAATCAGCGAAGCCGGGAGAGTGCCGAGAAGAATTCTACGGCCTGGTTCTGAAATCTTTTTTCCTCAAATTCCGACCGTGTGTGTACGTGTCAAAATATTTATACGCGCACACGCTCCACGGATTATTTGTTTGGTGAATTTCCACCAGGCACAGTCTGCATATATCTATCTACATATGTCCGTTTACCAGGAAGCGAATATACAAATCCCAATTCAGCCGATCAATTAATCTCTTAATTTACTTTAGCCGCGTGCAGGAACAGCAACAAAAGATACATCCATTAATGCAGATACTAGCGCGTATAAAATAAAGGAATCGCACAGATATAGAAATGTTTTCCTTTGGTGTTTCTGCTGCTGTTTCTGCTGCTGTCGCTATAAATATTTACAACGCCCGCCTTGTATACAAAGTGTAAATATGTAGATACAATCTGCTAGATACATTCGCGTACATAAACTGTTTCAATTGTTTTATTGGCCCGCTCATGGACAAAGCTGCGCAGAATTGAAAGTGAATCAAGTGCTATGAACCAGTCCAGTAAATAAAAAACTGACTTGCAACCACATAAACAGCCAAAATGTCTAAGAAACCACGTGGAAACATTCACAAGATACGAGAGTTCGAATTGGAGAAGGTGAGTGATCGGATATGATTCAATCCGAAATCGAATTGTAATACTCATTCATTTCCGCGCGACTTGTGACCCATTATTTTTATTTTTTTTATGGTTTACCGCGCCGCCATAAACAAATATGCAGATAGAGAAAGGTATTAAGAGTTCGTCTTGGCCCAGTCGGTATGATGGGCAATCTGCCAATTGTCTCTTCCCCGGGAAATCG

GTCTCTTCCCCGGGAAATCGTCTACAATACAACTGGGAAAATGCCATGTTAACGATTAGATTTCTGATCATTAAACACAAACAGCAATGGTCAGCGTCTCGTTCTCGAATACAGTAATGTTTAATAGGTCTAGGTAACGCATTCTATACATACATATATGTAGTATATAGTTGCCCAGTGCATTTGCACATTTAATTGTTCAGATATAGTTGAGGTGGGTCTTTAACGAGGCGTTGCACTTCGCAAGAAGCTCTCTTTGATTTTTTCGCATAAAAGGCAAATGTATTAGTGAGTCAATTAGTTCGTTAATAATGGAATTCTGTTTATGAACACGTGTTGCATTCTAAATCTAAATGAAAACAGCAAGTGGTGCAAATCAAGTATATATACTTTGAGTTCCAGAACATGAGGTTCAAACTTTTTAAAGGCATTAACTTTGGTGTAACTTCTACATAACCTATTTTTAGTTTAAGTTTTAACAAAATATGTGCCCACCAATATTCTCTATTTCAATTTAAAGATATTATGGTTCCATGAAGTTTTTCTATCCCCATTGAATACATCTATGCATATATCGCAGCTGTTTTAATATGCCATCTATATATAGATCCAGCTTGTGGACGAGTTTGATATCATACAGATCGTTGGCGAAGGATGGTTCGGGAAGATTCTACTGGTGGAGCATCGGGGATCACAGACGGAGATGGTCCTGAAAGCTGTACCCAAACCATATGTGACATTGCGTGACTTTTACCGGGAATTCCACTATGGACTCCACTTGGGAGTCCATCGACACATTGTGACCACCTACGACGTG

 

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