CR00651

CRIMIC CR00651

Gene botv

CG15110

Information

Donor Location Cytology FBti Stock number
pM37-p02R:19129937..1912994456B5FBti020061880617

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
no band no band GAGCACAGATGATGAGACTGTGCTGT GAGCCTCTCCCACCAGAACTAGATAT

Images

Construct Design Sequences

sgRNA sequence ATACCGGTTTCTCCGTCATTCGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
GCTTTCACTTTCAAGGCCCG GGAGAAACCGGTATACCTACGC CGGATACCGGAAGTGAACTT CCTTTCGCTGAACGCTATGC
 
Amplicon 5' Amplicon 3'
GCTTTCACTTTCAAGGCCCGGCCCACAAAAGCAGGCCCAAAGCAAAGAAAAGAATCAATGGGCGGGCAAAAGAAAGTGAAAATGTCACCGACAGTAAATATAAATAGAGGCTTTTACCCATATTCGAGTATTCCGTGCAGGATTCCGGCGAATGATCGATGTAACTTTGCGAGTATAATTAAAATCCGGAATCGGTTTTGTGCACTTAGGAACTATTTTCGTTTCCAATTAAAACTCGAACACAAGTGGTTAGAAATTGGCAGTCATTTCGCGACTATTAATAATCGGTTCAAGTGTTTTGCCGGTAATTACAAAAACAAACTATTTGAAACTATAAACCGCGAGGCGAAGCGGACGCACCGCGGATTTGGATGCGACTAAAATCACAAACACACTTTTGTCACGACAACTTAAAAATCCGACTGTTACGAGCTTTGAATCCGCTGTTAAATGAAAACTGTTGATTCGCTGTTGCGAATAATTCTGTTAGCAAGTGTAAGCAAAAACTTCATTGGTTTTTTGATTCAATTTTAATTATTTGATAGCGCTAGCAGTGAAAACTAAGATTACTAAGCAAGAAATACAAAACATTTGCTTTTATGGCTTCAAGATTGAAACTGTTATGTTTGAATAATTCGATTTTGGCGGGCTAACCGATGCCTTTGATAATTATCGATGCACCGATTACACGCATACTCCAAGTGGTGGTTGTGCAGCCCTGCATCGCGTTGCCAACGAAACGGACTCCACTCGCGATAAAGTGTAAATAAATGAGATATTTTGGTGGAATCGCGAGCTGAAACTAATGCCATGCCACCGGCGTACGACCTTGGCCATTCCGGAGAGGCGTACCAACCCCTGGACACTGGCAGCGGCGGCGGAAACGAAGCCTGTGCCCCGAACTCATCCTCCGCGCAAATCCGCCATTCGATGGGATTCCGGACATCCTGGATGCGGCAGTTCCGCCGCTACAAACTGCCCATGGTGCTGCTGATGCTGCTGTTTCTCGTTTCCTGCCTAGCATACCGCATCCTAAGCGTAGGTATACCGGTTTCTCC CGGATACCGGAAGTGAACTTCAGTGGAGATGGGAACTCAAATATTTGGAGCACCAAATATATTTATCTTATAATTAAAGATAACACTTAAAAGTAAAACAAAACAAAACACTTGTTGAATCAATAATAATCTTTATATATTGTTCTTACATTCAGATGTTTCTAAATATTTGAGTTTTCTTTATATATACGTGTATGTAGGTCAAGCTGTAAAACACAAATACAATCATTTATAAGATTCAAATTTAATCTATCATTGCTTTTATATAATTGAAATATTGTAAATATGTCCGATCTCAGATACTCACACAAGAACTACAAACTTTTTGCTTATCTTCTAACGTTATCAGCGTGTGTCAAAGATATTTACTTACCATAGATAATATTCTAAATATTGATCAACTGACAACTTTCATATTTTCCAGGTGGAGCAAGATGCCCCGCCACTGGATCTGCATCGTAGTTCACCGCTGCTAGATGCCTACGAAGATTTCAGCGCCATGAGAGCAGGAGATCTGAAAATGCGCATCGAGGAGATGGTTAGAATCAAGGTTAGTAGCCGAGAACTCCTATAAATACGTAGCCAGAAGCTCAAACCTCTTCTCATCCAGAGCACCGTGTCCGTGGAGTTGCGCGAATTGGAGTCCCGTCGCCAGAAGCTGCAATCGGACATTAGCCAGTACAACCAGAAGATCGAGGAGCTCAAACAGGAACTGCTTAGGGAGCAAACAGAACTGGAGCGCCTCAAGATCTCCGTGGAGCAGGCTCAGGTGGCCCAGCGGGAGGCTGTTCAGCGGAATACACCGGACTTGGCCTTGCCCCGCTCTCTTTTACCCAACACACTGCCAAGAAAGAGTAATCCCATCACCGGAGGGATGGCGGCCAGCTGCGAAATGCACAACTGCTTCAATCACTCACGCTGCAGTCTAACCTCCGGATTTCCTGTTTATCTATACGATCCGGATGAGCATAGCGTTCAGCGAAAGG
 
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