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CRIMIC CR00654

Gene Mapmodulin

CG5784

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:17869566..17869595	54F4	FBti0200621	80618

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	no band	CACACGCGTACCACGCACAAAAGATA	GGGCTTCAGGGTTTCCAGATCCTTTA

Images

Construct Design Sequences

sgRNA sequence	CGTCGTGACATATTGATAATTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CCTCGCAGTCTGGTCACATT	TGAACTTGCACAGCCTCCAA	ACGACGCCACAACAAAACC	CCAAACGCTGTGTGCATTCA

 

Amplicon 5'

Amplicon 3'

CCTCGCAGTCTGGTCACATTTGTAACATTTTTTGACGCAAAGCGAACAGAAAAAAAAAATGGAGAACAATACATACGCAAAAATCGATCAAACGCTGAGAAATTTTTTTTTGTTGAAATGCAAAACGAAATTACATGCAAAATAACCAATTTGGCGTTAATGCAAATGACTTGGTAAACATTGCTGCTGCTCCATCGCCAAATTGCATATTTTTGTAATGCAGGCGACGAGGAAAAAGTGCCAACTCGCGTTTATGTAACCCCAATATATGTGTGTGTGCCACAAGCGTGTGTATGTGTGCGTGTGCTTCGACACAAAGCAGAATGCGCAAAGAGAAGAATGACGAGCAAAACGGAATTATCAAGAAGTGGAAGGGAGATGGCAGGGGAACAACAAGACTAGCCGGCACTCGCAGCAACAAAACAACAACAACATGGTTGACCGTGACGTAGTTTAGTTGTTGCGCCTGTCTGCGAATAGTGGGTGGGGGTGAGAGTGGGTGGTGTTTTGTGGTCTGATAATCTACAAATGAATAAATGTACACAACGTACGCCTTTTGGAGGCTGTGCAAGTTCA

ACGACGCCACAACAAAACCAACAACAAAGAAACAAAATGGGTGCCTTCAGAGTCCGTCTGTCCGTCTCTGTCTAGCCGAGAAACAGTTATGTGCTTACAGCGGACACTTTCTAACCTGAATTATGGTTCAAATTTATTTTATTTCTAAGTGGCAAGAATCTTGGTATATCCTAAAAGAAAATTAACTGTAGCTCAACACAATTTTTAATAATTATCAACAAAATTGAATATGAAAATATAAAATTGTAAATAAAAACTGACTGCAAAACCTAATCTGTATTCGGTTTGATATACTTTGTAATATAAATAACTTATTGTTTGATGAACTTCAAAATCATGTTTTTGGTAATGGAATCAATTTAACTATGTTAAGAATCTTTTTATGGATATGGTGTAGTTTAAACAAAAAATTTAACTCTTGGAAAGGAAACAAAGTGGCACTTCCCCAAAGGTATAATTTATAAAAATTGGAAATGAAAAAAATGTTCGTATGAAATTCACGGCTAAGAATTCGGTTGTGCTCGATCTATTGATCGATCTAGTAAGCACCAAAGTGGGGAATGCAAATGATCTAAAATATAGCTAACAGTAGAGAGTGTCTACTGTATTTGAGCAAATGAGTGGAAATGAATGCACACAGCGTTTGG

 

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