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CRIMIC CR00657

Gene CG6805

CG6805

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:17006760..17006783	53E4	FBti0199436	78974

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TAGTTGTGCTGCGGTCACACTAAGCT	CGATGTGAGATAGGGAATTGAGGAGG

Images

Construct Design Sequences

sgRNA sequence	AAAATCAAATGAACTTTATATGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CGGACTCTCTGAGCGATCAC	TGGGTGCTAGAGATAAGGCG	TTGCAGTTCGTGGAAGGCA	TGCCCAGGCGAATGTTGTAT

 

Amplicon 5'

Amplicon 3'

CGGACTCTCTGAGCGATCACCAGTTCGTAAAGGTGGACTCGAAGCAGCTGCAGGGAATTCTTATAACCATGTTCGCACAGCACAAGCACATCCCGCACATGAAAGAAATCGAGACAGAAGCCACGCGCACGGGACTTGGAGGGCTGTGGGGCAACAAGGGTGCCGTAAGCATTCGACTTTCGCTCTACGGAACTGGCGTCGCATTCGTCTGCTCCCATCTGGCGGCGCACGATGAGAAGCTGAAGGAGCGCATCGAAGACTATCACCAAATCGTGGACAATCACAAGTACAATGCGCAGGGATATCGCCGGATATTCGATCACGACTTTGTCTTTTGGTTTGGCGATCTTAACTTCCGTCTCTCCGGCGATATGTCCGCTTGGGATGTCCGCACGGATGTGGAGAACCAGCGATACGCTGATCTGCTCAAGCTCGACCAGTTGAATCTGCTCCGGGAGAAGGGAAACGCCTTCAGCTTGCTGGAGGAGCAGCAGCCCAACTTTGCGCCCACTTTTAAGGTAACAATCTAATATACAATCTTATTATCTCTACAGTCAAAGTTTGCCTTGAACTTTCTAATCTTAATCTAAATAATGTATATAGGGGTGGGTGTAATTAATTTTATCTGCACTTGAATTTTTAATTTCCGCCAATCACTTCATCAATCAATCACTTCATTCGGCTCCACAGATTTCATGAGAAAAGCAAAATCCAACTAATTTATGAGGCAATATGTCGAATGTAATTAATTCGCCTTATCTCTAGCACCCA

TTGCAGTTCGTGGAAGGCACTAATGACTACAACTTGAAGCGGCGACCCGCCTGGTGCGATCGGATTTTGCATCGCGTGCAGAGCAACATTTATCCGGGCATTACCCTGAGTGCCAACCAGCTGTCTTATCAGTCCCACATGGACTACACTCTCTCCGACCACAAGCCCGTATCGGCGACATTCAACTACAAGGTCGAGGCTGCCAACCAGACCTACACCGACGAGGAGCTCCACGAAATGACGCACGGATCTGCCTCATCTCCGGCGACGCCAAATGTTAGTCTATCCTTCGCATTTGTTGTTTTTGTAGCTGTGTCCTATACTCAACTTTGATTTCGCTTCTTTTGTAGTTATTTATCGCTGAACTAGAGCAATGGTAGCGCCGTCATGACGTCACTTAGTTGTGAACTTTTAAATCTCTTTGCATGCTTTTAGATTATATGCGTAGACGAGTTGTAGAGTAATTAAAAGACTTCCTTTCAGAAGGAACAAGAGAAACGAAAATAATATCAAGTACAATGTGATGAAATCTCTGCTAATTTAGCAATCATTTAAGCAATTCTCAAGTGATCCATGGAAATAATGCAGCTTCCGAGTATATATACATACATATGTTATCACTCAATTAAGACGATATTAATTTAACGATTGATTGCATTAAACAAATGTATTTTTGTACACTAAACTGTTGATCGAATAAAAAAAACTATTACCTCTCGACGTGACTAATCTTTTTTGTTACTGAGTCAATTAAAATGATACTCTGTTAAGTATTTGTATTGAGAAACCTGTTAATACAAAAAAATAAATAAATCGATATGACGCATTTTTGTTTTGTTATTGGAGTAACTTTCCCAGATAACAACGGATAAGACTTCTTAGAATGGTTTTGGGTTTATTCGATGCTGATGACAGCATTTTCGTTTCTTTTATTAGCTATTAATGTTCCTTGGGTCGAACAGTTCTTTATACAACATTCGCCTGGGCA

 

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