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CRIMIC CR00658

Gene CCT8

CG8258

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:8906732..8906732	44F5	FBti0200622	80619

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	OK	CACTGAGCGTTCGAGAAATCTGCAAG	ATTGTGGTGTCTCCCAGTTCCTCGAT

Images

Construct Design Sequences

sgRNA sequence	TTAGTCATCCTCATGGAACGGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GACGGGGCAGGAGAAAATCA	GTCATCCTCATGGAACGGGG	ATGACTAATCACGCGCCTCC	CCACTGAGCCCTTGTACGTT

 

Amplicon 5'

Amplicon 3'

GACGGGGCAGGAGAAAATCACGATCTTGGCCTTCTCTGCATAGGTCACATCGCCTTCTACGAAGCGCTTGAAGACCATGCCGCGAACCACCTCAGACTTGGTCAGTCCGCTGCCCAAGATCTTACAGATGCGAATGTTGTCCACGTTGAAGGTGCCCTCATCCGGCAAGATGGAGACGCAGGCCTTGCTCACCAAATCGTTGAGGAAGTCCTCCTGGCCGTACTGCTTCGACATGATCGATGTGCGGAGTACCTCCTTGACCTTCTCCACATTGCGATAGTCCTCGATCTTGTGGCTCACCAGATTGGGCAGAATCTCCAGAGCCTTCTCCAAGGCCTTTTCATAGCCGTCGGAGATCTCGGCAGTGGTGATTCCTAGACGCAGCAGCTCCTCAGCGGACTCCAACAGAGCTCCGGCCAGCACCACCACGAAGTTTGTACCATCGCCCACCTCGGCATCCTGCATCTGGCTGGCCATGACGATCAACTTGGCGGCTGGATGCTCAACATCCAGCTCCCGCATGATGGTGCCGGCATCGCTGGTCACGAACTGCTTCTCGATGTGATTGATGATCATCTTGTTCATGCCATTTGGCCCATAGGCAGAGCGCATGGTCTGTGCAAACTCCTTGCAGGCGCTGATGTTGCGGAACACCGCCTCCTCCAGCCCACTGTACATCTGAGGACACAAGTAAAGCCATTAAATTAGTCATTTTTCTTTCATATGTCGAGCCCCGTTCCATGAGGATGAC

ATGACTAATCACGCGCCTCCGCAAGACGGCATGTTTTCTTTTAAGCCGAATCTCAATGAAACCGATGCCCGGCGGACTCCTCGCAGAGTTATTCCTCCTTGTTGCACTTACCCGGGCGCCATCCTTCAGCATCTGCGACACACCGGGAGCCTTGGGAACGGATAAAGCCATTTTTTAACACAGATTTCAATTTCACACGGAGTCAGTCGGTAAATTCCAGCTATAGTTATAACCTCAAAGTGGCCGCGAACATTCTGGATGGGATAGCGATGGCACTTGCCTATCGATTATCGTAAACGGTGACTGGCGCTATTTTATTTTTATTTTACATTTGAATTGATCTTTTTCAATAAGGTAACGATTCTCAATGGTTTATGATGAATAGCATGCAATTAACAATATAGTTCAGCAGTGGAGTTTACGCCCCAAAGAATCCTGTAAATCGAGTTTATTTCTTGGTCTTTGAACGCATTTACTTAAATAATCCAGGATAATGTATATAAGTTACTCCAAATAAGTAATATTTACAGTTGCAAAAGAATTTACTCATTTTAAGTTTTGAGGAAATTTTAAAACAATTAGAGTAAAATATTTTATTATTTAATTTAAAAACTGAAAAGTTTATTTAAAAAATTCACATTCCCATTGGTTTATTATATTGTTCACTCGATGAATTTTGTTTGCATATAATTTGATAAATCTTTCAAGGGTATAGGAAACGTCGGTAAATGCAATTCAATCGTGAGTCGTGCGTGACATTTGCGTGATAGATTGCATTAAGTGCGACGAGTGTGAGCCGATGTAAACACTAGCCTTAGCGCCCATCTCTAATTCAAAACATAAAAAACGATGTCGCTGGGCAAGAAGCACGTGTTGGGATTTTCGCCCAATGGTGGCCAACTATTTGCCTCGGTGGACGAGTCGGGAGTTCTGCGGATATGGAACACAGAGACGAACACGCTGTATCAGGAGTTTACGCCCAATCTACAGTTATCCGGGTCGTGCACAGCTCTCACGTGGGTTTTGGTGGCCGGTTCCCGGCCCAAGAAGTCGCGGAAGAGCCTCCAGGCACAGGATGCAGCCGGTGATCAGCTTCACATTGCTCTGGGAACGTACAAGGGCTCAGTGG

 

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