CR00662

CRIMIC CR00662

Gene GABA-B-R3

CG3022

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:753734..753756	21E2	FBti0199438	78976

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TATGGTCGACTGCTGTGCAGAGTGAA	CGATGTGGAACCGAAGGATACCTTTC

Images

Construct Design Sequences

sgRNA sequence	GTGAGAATGTCATTTCTACGTGG
sgRNA2 sequence

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCGCATAATTCAACCGGTCC	TCACCTGAGTATCGTTGGTCAC	TAACCGTATGCCCCACGTTG	GAACCGAGCGAGCTGGATTA

Amplicon 5'	Amplicon 3'
GCGCATAATTCAACCGGTCCAAGGGACCAGATACGGTCCATGGCCGGCCGTGGGACTGAGGCTAGTCCTGGCCCTTGCCTGGGCAACGTCGGCAGCGGCTGCCATGGAGTCATCAGCCGAGCTGCAGGCCCTGGGCTACGAGGCAATTAGGCCAGGTGCTGCCTCAATTAGCACATCCAGCCCATCCAGCTCGCCACCCGGAGAATCGGCATCGACTGTGACTGCAGGGGGGACTCCGATTCCACCGCGCTCCGATTGGAAGTACAAACGGACGAAAGTCAAACGCCGGCAGCAGCGCCTCAATTCGCACAGCAATCTGCCCGGAAGCACCAATGCCTCCCACGCCCACCACCTCCTCAATCTGCCCCCCAGGCAGCGATACTTGAAGGTCAACCAGGTGTTCGAAAGCGAACGCCGCATGTCGCCGGCCGAAATGCAGCGCAATCATGGCAAAATCGTGCTGCTCGGACTCTTTGAGCTGTCCACATCGCGGGGACCACGTCCGGATGGTCTGAGCGAATTGGGAGCTGCCACCATGGCCGTGGAGCACATCAACCGCAAGCGCCTGCTGCCGGGCTACACCCTCGAGCTCGTGACCAACGATACTCAGGTGA	TAACCGTATGCCCCACGTTGGGCGCCACTTCTAGGCGTCGCTGTTCCATTACGAAATTGAATTTTCATTCAGTAGCGCGTGCAACATTAAAACTTAATAAGAAAATCTGCAACAACAAGAACAGTGCGGATAGCAGCTGCGGCTGTGGCCCTAAAAGAGAGGCAAATATGTCATCAACCTTCCGTCTTTCGCGCTTTTATTGCGATGAGTCGTTGCCGGTTTTGATGGGGCACGCAAATGTACAGAACACTGTCACTGAAGAAAAGCAATGCCACTGACTAAGTTACCAACAATGTAAACTATTTACCTGCAATCATTTTTAACTTGGTGCAGTAGTGTATTAAAGCTACAATTAATTGATGGTAAAAAAAAGAAAATGCATAATAGTAGTTTAGTTTCTGATAAATTAAACCGTCAATACGTAATACCTCGACTGTGATGTCAGCACTTAGTGAACTAGGTTTTCAAACGATTTTTTTAAGTGTATGCATATGCACATCGACCTTAAACAGACTGTCATATCTGGAGCCGCATCTCCGGCTGTGCCTCTCTAAAAGTCGACATTTTAAAAATGTTTATTGCGCCATCATGTTGCCTTTTTATTGCGTCGAAACGAGTCAGCGAGCAACGGCCTCTGCCACTGCTATTTTCCCTGCCCCCTCTATTACTCATCTCTGATGTGGTGTCCTTTGCTCCTCCGTTTCTTCACACATTTCTTCTCCGACAATCAGTGTGATCCTGGAGTGGGCGTGGATCGCTTCTTCCACGCCATCTACACACAGCCCTCGACGAGGATGGTGATGCTGCTGGGATCGGCCTGCTCGGAGGTCACCGAGAGCCTGGCGAAGGTGGTGCCCTACTGGAACATCGTGCAGGTGAGTCATCTGCGACTGATTAAAATGCAAATAATCCAGCTCGCTCGGTTC

Amplicon 5'

Amplicon 3'

GCGCATAATTCAACCGGTCCAAGGGACCAGATACGGTCCATGGCCGGCCGTGGGACTGAGGCTAGTCCTGGCCCTTGCCTGGGCAACGTCGGCAGCGGCTGCCATGGAGTCATCAGCCGAGCTGCAGGCCCTGGGCTACGAGGCAATTAGGCCAGGTGCTGCCTCAATTAGCACATCCAGCCCATCCAGCTCGCCACCCGGAGAATCGGCATCGACTGTGACTGCAGGGGGGACTCCGATTCCACCGCGCTCCGATTGGAAGTACAAACGGACGAAAGTCAAACGCCGGCAGCAGCGCCTCAATTCGCACAGCAATCTGCCCGGAAGCACCAATGCCTCCCACGCCCACCACCTCCTCAATCTGCCCCCCAGGCAGCGATACTTGAAGGTCAACCAGGTGTTCGAAAGCGAACGCCGCATGTCGCCGGCCGAAATGCAGCGCAATCATGGCAAAATCGTGCTGCTCGGACTCTTTGAGCTGTCCACATCGCGGGGACCACGTCCGGATGGTCTGAGCGAATTGGGAGCTGCCACCATGGCCGTGGAGCACATCAACCGCAAGCGCCTGCTGCCGGGCTACACCCTCGAGCTCGTGACCAACGATACTCAGGTGA

TAACCGTATGCCCCACGTTGGGCGCCACTTCTAGGCGTCGCTGTTCCATTACGAAATTGAATTTTCATTCAGTAGCGCGTGCAACATTAAAACTTAATAAGAAAATCTGCAACAACAAGAACAGTGCGGATAGCAGCTGCGGCTGTGGCCCTAAAAGAGAGGCAAATATGTCATCAACCTTCCGTCTTTCGCGCTTTTATTGCGATGAGTCGTTGCCGGTTTTGATGGGGCACGCAAATGTACAGAACACTGTCACTGAAGAAAAGCAATGCCACTGACTAAGTTACCAACAATGTAAACTATTTACCTGCAATCATTTTTAACTTGGTGCAGTAGTGTATTAAAGCTACAATTAATTGATGGTAAAAAAAAGAAAATGCATAATAGTAGTTTAGTTTCTGATAAATTAAACCGTCAATACGTAATACCTCGACTGTGATGTCAGCACTTAGTGAACTAGGTTTTCAAACGATTTTTTTAAGTGTATGCATATGCACATCGACCTTAAACAGACTGTCATATCTGGAGCCGCATCTCCGGCTGTGCCTCTCTAAAAGTCGACATTTTAAAAATGTTTATTGCGCCATCATGTTGCCTTTTTATTGCGTCGAAACGAGTCAGCGAGCAACGGCCTCTGCCACTGCTATTTTCCCTGCCCCCTCTATTACTCATCTCTGATGTGGTGTCCTTTGCTCCTCCGTTTCTTCACACATTTCTTCTCCGACAATCAGTGTGATCCTGGAGTGGGCGTGGATCGCTTCTTCCACGCCATCTACACACAGCCCTCGACGAGGATGGTGATGCTGCTGGGATCGGCCTGCTCGGAGGTCACCGAGAGCCTGGCGAAGGTGGTGCCCTACTGGAACATCGTGCAGGTGAGTCATCTGCGACTGATTAAAATGCAAATAATCCAGCTCGCTCGGTTC

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