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CRIMIC CR00666

Gene CG8635

CG8635

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:8631584..8631610	44D5	FBti0199439	78977

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TAAATGAGCGCGAGTGAGCAGCAGAA	TCCAGTAGTCGATTGGTGGAGGCAAT

Images

Construct Design Sequences

sgRNA sequence	CACATCACGGATTTACAAAGAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GGCATTATCGTCGTCGTCCT	ATCCGTGATGTGGAAACCCG	GGCTTTCCGGTTACGAAGC	ACTCGCTCGCACTTGAGATC

 

Amplicon 5'

Amplicon 3'

GGCATTATCGTCGTCGTCCTCCCTGTTGTAGACGTCGAAAGCAGCGTCGCCCTCCTCCATGGGTCCGTCGTCGACCAGATCCGGATTGAAGCTGAACATTTCGCGACCAGAGATGCCGAACTGTTTGCCCTTGCTGAAGTCACTCTTCTTGCGCTCTTCCTCGGCAGCCAGCTTGGCCTTCTTCTCGGCAATCTTGCGCTTCTTCCAGGCGAGGAAGGACTCGAGGGTGACGCGTGTCTGGTTGGGACCGAGGGCAGCGCGCTCCTTCTCAATTAAATCGACCAGCGAGATTTCCGTGGGCTTTTCCTCCTTCTTCTTATCGCGCTTCAGCACATATCCAGCTGGCAAGGCGTGACGGTAGATGCACTTTCCGCCGTTGGGGCACTCCCAGAACCAGCCGTACTTTGATTTTTCCACAGCTTCCAGGAAGAATTTGCAAATCTGAAACACAGGGGAGGTTAGTTTTGAGTTTTAAGCAGTTTTTTAAACTTAGACCCACAATATCCGTGGTGGGACGTTGCTTTTCGCCGGAGCTCTTCTTCTCGACCACCTCCTTCAGCTTGGCATCGTCCCAGTTGGTCATCGGATCGTCCTCGTCGCGCATGTCCACATAAATGGACCGCTTTTCGACCTTGTTCTCCTGGGAAAGATCGTGGGAGAACTTGCACTTGTCGCCCTTGGTGCAGGTGCCCTGTTTGAAGAAGGCGCAGACCACCGACTTGGGATCGGTGCCCTTCTCCACCTTCTGAGTCTGTACCGGCTTGAAGATCAGCGCCATCTCGCGCTGTTCGGCCAGCTTCTTCTCCTTCTCCTCCTTGCGCTTGTCGCCATCCTGGCGAGGATGCTGTCCGCCGGACTGGACCTGCTTCTGCACCTGCTGGATGAACTTCTGCTGCTTGTTGCCCTTCTTGTTCTTCAGCCCGAAGGTTTTGTCCTGCCGATGGAATTACACTTGGTCAGTTCAATGTGGATCTTATTGAATTTCTCCCTGCCGGGTTTCCACATCACGGAT

GGCTTTCCGGTTACGAAGCCTACCTCAATGACTTTCTCCTTCTTTTTCTGCTCCGTCTTCTTGCTGGGACCTGGTGGCGCCTTTTTGGGTGGCATCTTCGGGCGGGATACGGGAATTTAATAACTTAAATGTAGAAATAAAGTTCAAACGAAGTTTTTTGCACAACCACGCTGGAACAAGAGGCCCTGTCAGTGTGACCAGAGTGCGGATGGCAGCATATCTCAGGAAGAACCGGTTATCATTAATTAATTTAACAATTGTGCTTTTAAAAATGTAGTAACTGAACAAATGATTTCTTGGCCAGCAACTTTTAATTAATAAGTGGAGTGTGCATGAATTGCACACTTTTAAACTGTGTGGTTTGAAAAAGAGCTAAGGTTTTCAGCGCACACTTAAACCACTCAATCGATGTTTACATGGGTTCAAAACTTGGCTATTTAACTTATTTCTAATATTTAATATCATACATTTTAAATCATCGTTTGAGTTACAACTTTTAAAAGACATATCGTTAAACATATATAGTAAGTGGCTGTTTCAGCAGCATAGCGACTATTTTACAGATTTTACACGAGCTCCATCTAATGGCGTCGACATTACTTAGAATACTAATGCCCTACAAGTTACCTATAATTTATATCCGATCTATAGAGCGATTCGAATAATTTTTGGTATTGGAATCTAGCTGCTTACTCCCATCTCATTAGCTGATATAACTGATATACAGGCGAAGGGAAAGACGGACATGGCCGGATCGACCTAGGTAAGGGTAAGGATCCTGGTCCAGAATATATATACTGTTCCTGTGACTTAGCATTGAAAAAATCTTGTATATATAGTATTCCCATTAAAATACCTTAAACCCTCTTCTAATATAAATCCCATCTTCCATTGCTCAAAATTGATACTTTTTTTTCTCACTGTACCATCTCGCTCACCAACACACATGCAGCAACGAGAAAGAGAGCAACGACGTGTCTCTTCGTGGGTGGCCCTTAATTCGACGTCATCTTCTTGGGTGGTCCATATTAGCCGGCCTGCTCTCTCCCCCCTTTTGATCTCAAGTGCGAGCGAGT

 

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