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CRIMIC CR00668

Gene Rchy1

CG16947

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:6462327..6462327	26D6	FBti0200626	80621

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	no band	ATCGCTTTGCAGCATGCTCACGTAAC	GTCGATGCACAGCTCGAACTACATGA

Images

Construct Design Sequences

sgRNA sequence	TTGTCTTGATCGCTCGCGTTTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCTGCTGCTGCGATGATAAC	TGATCGCTCGCGTTTGGATA	CGAGCGATCAAGACAAACGC	CAACAGGCAGTCCAACTCCA

 

Amplicon 5'

Amplicon 3'

GCTGCTGCTGCGATGATAACACTCGAAGTACAGAACCGAACCGGAGAAGAGTCTTCGAATCCGGTTTCTTTGTCATTCCCCCCGGCTTTCATTGCTGTGCTTCACTAACGATCGTATTTTAATGATCGTTTGCATCTGCAATTGGCTAACTCCAGACATCTCAAATTTATTTAGCCAAGACGAAACAAAGCTTCTAACACAAATTGATACAAGATTCGCGGGAACAGGCGATATCCATGGGGCATTGCTCCACTTGGGGGATTGCACATACTGCTCTCAGCTTATGTCCACATTCATGTATACATTTATTTCACGCACTGTCGGGCGAAAATAAATATTTGACTATAAAATAACCCAGCTGATTCAACACTCAATGGGCACCTCATTAGTGCACAAAACCCTCGCTTATCCGAATTTCTCGCGGTGCACAGACGAAAGCAAAAACCTCTACATTCGAATTTTATTAAAATGTGGGACAGATTGAAGCGTGTGTACGGATTTACGTGACATTTTTTGGAAAGTATTGTAAGGCCATAGCGCATAATACAGAATTTTTGGGGAATTAATAATTCATATCGCGAATTGGCTGCTTTTCAACAACAAATAAGCGCTCACTACTGTTGGAATTATAAGTATTTAAATATTTACATTTAACTTTCTTTTTCTTTATTTAATAATCCAGGTCATTCACAAACAAATCTATCTAATCAAGTGCAGCTGCAGTCTTCTCTGCATGAGATACTTTATACATGAAATGGTTACCACTTTCCCCAGCACTGAGTTACTTTTCCCACCTTCGAACTTCTGTACTTATCACCTTACGATAATTATGTCTGCTGGCTCTACTAATTGTTAAGGTAAAGAACCTGAATATAGCCGTGCTACGATTGCTTTATATGCACACACAACATTTTTAGATGCCCCATAATTTGTTATCCAAACGCGAGCGATCA

CGAGCGATCAAGACAAACGCATTGTTTGCCCAGATGAATGGCGAATGGAGGTGTTAACTGGCCAGTGGCCACTCGCCTCCTGCTCAGTTCCTGCTCAGCGGAGCATGCTGACCAGTTAGTTTTTTGGATGCCGGACATCGTCAAGCTGTCCACACAAGCCAAACACTTGCCGTGCAAATAGCGATGCCCTCCAATTGAATTCAAAATCTAGTCAGCAGACAGACACAAAAGAATCTTCGCCAAAGGTGTCTGTTTAGAGTTTAGCCACTTTATTTGTTTAGGAACGAATAAATAAAGGTTAAATGCGCCCATTTTCAGGAAGGTAGCTGTGTTGGATAGACACACACACACACATAGATAGATAGATGGATAAATAGGCATACAAATGCCATTTAGAGGCGCCATCATAATCTTCTTGAAAATTTTCCAAATGGGTGAAATGTTTAATGGCTTTATCACTTTGAATTTATTTCAATGTATTTTTAGGATTCATTTTATAAAAGTGAAATGACTGATCCGCTTTCTAATCATGACATAGGGGTTCCAATTGCATAATTCTTTATCTGATGACCCAGGAATTGAACTATACCCGTATAAAATAAAAAAAGAACTTTCAAAATTGAAGTTTGTTCTTATATCACATAAGTTGACTGGTTTCACATTTGAGGTTTGAATAAACTAATCTCACTACAAGTAAGACTGCTAGATACATGGATCCGTCTAGTCTTTCTACGAACATCTATCTACTAATCTTAAGAATGGTTATAGATACTACGATACACTAAAAAAGGATTAAAATTTGTATTGATATACATATCAAAGAAGTTCAAGTGTATAACTTTTATAACTTTTCTGGGGCTTTTCCTTTTATGTAATGCTTGGAAAAGCATCCATAGATAATATGAATGGAGTTGGACTGCCTGTTG

 

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