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CRIMIC CR00670

Gene CG14591

CG14591

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:6037579..6037579	42A8	FBti0199440	78978

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGTGGTTATCGTACTACCCTAGACGC	GAAACTGCCCCAATTCCCATGACCAA

Images

Construct Design Sequences

sgRNA sequence	TGCTTAAGGGTAGGGTGTATAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GGCATGGTGTACTCTGCTGT	AGGGTGTATAGGAAAACGCCA	ACCCTACCCTTAAGCATAGATGT	CCAGTCATGCCTGCAGGTTA

 

Amplicon 5'

Amplicon 3'

GGCATGGTGTACTCTGCTGTAAGGGACGCCATTTCCCCTGCGTCATGCGGATGTCCATCGTCCACCGCTTCCTGGTTGCTAAATCGGTGTCGCAATATCGCCTCCTCCTGCATGGCATAGTCACCTTGGGTTAATTGGTGATACTGGGCAGCCTCGCCCCTCGGCTCCGGCATACTTCCCGATCCGGTCAGGCCGCTGCTGCTCCGATTCCGGCGTCCGAACAGCACCACTGTGCCCTTACTCAGCAGCGGACAGAGCAGTGCTTGGTGGCAGCACGCAAATATCCGGTAATTCGAGCCCTGGAACGGATCTGACATAGCCGCACAGAGCATGTGGTCCAGATTGATACCAGTAAACTGCAAAGAAGGGAAGGTATTAGTGGCAGGAGACGTTTTGGATGAAATACATAATGTGCTAAATATTGTAAATAATGAATTTAAGTATACCAGAAAATGCCATCGTTTCAATTGTTTAGGCCTCGAAAAATCTGACCAAATAATGCTAGAATTTATTTACTGGCGTTTTCCTATACACCCT

ACCCTACCCTTAAGCATAGATGTAAAACACTTTTGCTCGAAACGAATGTAGAGAGTAATTTTAAGTTTAACGCCACTTACAATTGAGAGCGGAGAGAGAAGGATGAAGTGATAAAACAGCATGAAGGCGGTACCTATATGTGACCAGTGGAACTCGCTGAGGTCCTCGACAGTGTACGCTCCTGAAAGAGAAAACAAGATTACTTTTCCGACCAAATAGATGGCGTCTCCTAGTTACCGCTTCGGATAATGTAAATCGGTATTATATAGAGCAGTGCATGCTGGATCCAATAGGTGGCCTGCTCGAAGGGATATGTGCGGCCCTCCACCTCGGGGAAAAGAAACGCCAGAAATGGGCCGTTGAGATTGCTCATCTGTATACGAAAGAGCGCTGTTGTCGTCTTCGTCGGCTTGGCGGCCAAAAGATAAATCTGTAAATATCCCATGTATTTTTAATTGTTCTACGACAATCCACATGACAAATTGTTAACCTGCAGGCATGACTGG

 

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