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CRIMIC CR00674

Gene CG11807

CG11807

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:15202757..15202785	51E5	FBti0199442	78980

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AATGTCCATCGACCTCTGACAGCACT	AGCGGATTTCCAGTAAGTCGAAGGGT

Images

Construct Design Sequences

sgRNA sequence	GCATCTATCATTGATGCTCTCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ACCAAATCTCAGGGAAGCGG	TAGATGCCTCACGAGTTTGC	TGACTAACCGACGATTGGGT	TTGCCTGTCAGGTCGAGTTC

 

Amplicon 5'

Amplicon 3'

ACCAAATCTCAGGGAAGCGGAAAGGACTGCGATGGCTTGCTATTACCGCCAGCATGCGGACACCACGGTGACGGTTCCAAAGTTTAGCAACGAGAGTTCCAGCGGCGGAGTGACCTACTACGATATCAAGGTGCGAGTGGGCAAGGTGGAATGGCTGGTGGAGCGCCGTTATCGGGACTTTGCAAATCTTCACGAGAAGCTGGTTGGCGAGATCTCCATCAGTAAGAAGTTGCTGCCGCCGAAGAAGGTACGCCCCCAAACATTTATTACACATACGTATGTAAGTTATGAATAATTTCCTATTTTCAGCTAGTTGGAAACAAGCAGCCCTCCTTTTTGGAGCAACGTCGTGAACAGTTGGAAATTTATCTTCAGGAGCTTCTCATCTACTTCCGTACAGAACTGCCGCGAGCTCTGGCCGAGTTTCTGGACTTTAACAAGTACGACATTATTTATCTGCTACAAGATCTTGCCAAGCTGTTCAATGAAAGCGGTGATGCTCTGCTCAGCTCCAAGAAGGAATACAACCTTTCAGCCCTAGAGGTAAGATATAATTATATGTAAGTGTTTCCTTTGCGTTTTTTGTTAAGTTCGAATATACAGAAAAAATGTCAAAATTATTAGTATTCAACTTTATCCAAACCATCCCTTTGCAGCTTAGAAATAACTTTAAAAGCAAAGTTAAAGCTAGACCCATTAGTTACTCCCTGGGTACCCTCCACAAGCATCACACAAATTTTTCTACAGAGCTGGAAAATACCATAAATTCTGAGAGCAATTTTAAGAATATCAGTAACCTTATAAAGTTTGTTCGACAACATAGTAAGTGGTACCGAGATAATGACTGTATTAATCGACATTATTTTAACTACATATTACTGGATCCTCGAGTAACAAATAATCTAAAAGAACGGGCCAGTCAACTTCACTTAATGGATGTTTGGTATACCTTTCTAAGAGCAATATTTTATGTAGGCAAAGGCAAGGCCACCCGCCCCTACGTTCATTTAAAAAACGCCCAGAAACTGGTAGATGAAACCAACAACATCACATTAGTAAAAGATCCCAAATTGGCGTTAATTGTGTCCATATGGAAGGCAAATCGTGGAGTCTTACTAATACGCGCTTTTCAGGGAATCTCGTCACAAGATGCGCAAACTCGTGAGGCATCTA

TGACTAACCGACGATTGGGTTTTTACTATGGTCGAGCTCGAAGTTTATCGGATAAGGAGAGAAAATATCTGGGGATAGCTTTACTTTACAAATTGATGATGAAGTTTCTAGCAAAAGAAGAAAAAGAACTATTTCCTTTAAAGAACACCAAGGCTGCAATGGCAGCTTAAATCAATATAAAATATACTTATACTACTTATTCTAAGATTGTTTTTTATTTTTTCCCCTTTCTACTAGGTCTACGCTATTAGTGAGCGGCTTAGTCTTCCCTGCCCACCGAGCCTGGATCGCGGTGGAAAGTACGACTTCTCGCACGTGCTGGACTTTTGCACACAACTTGTCGCTTTGGTGGTCACCCCGGTCAAGGACAATGCTAGCTATGCCCAGGATTACAATACGGTTGATGTGCCAATCGATCGCAGCAATATTATTCCGAACAGACTAAGTTTTAATCTAAATGCCTTTCGCAATCTCAAGACGCTCAAATTTTCGGCCTTGTCTACCGAAAACATTGTGGACATCGAACTTCTGAAGCCGACTCTCCAGACAATCTGTGTTCATAACACAACCATACAGAACATAAATCAAGTCCTGCTCTGCGACAATCTGCACAAGCACTGCGATGTGCCAAGCCTGCTGCCAGAGACTATCCTTGCATCCCCCTCCGGTTCCGGTCCCTCCACTTCTAATGGTAGTGCCTTGGTCAGTGCGGATGCGTGGCAAGAGATAACAGAACTCGACCTGACAGGCAA

 

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