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CRIMIC CR00675

Gene ckn

CG12424

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:14976989..14977000	51D6	FBti0200630	83184

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTAATGATGGCTGCCGCTGCTGTTTA	AAGTGCCCACCTTAATGAGCACCACA

Images

Construct Design Sequences

sgRNA sequence	GATTAGAGCGACGGGTGGGTAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TTCGCCCATTTGTTCGAGGA	CACCCGTCGCTCTAATCAG	TCATGAGAGACACCAGCCCA	GGATGGCGTACTGGGTATGG

 

Amplicon 5'

Amplicon 3'

TTCGCCCATTTGTTCGAGGATGGAGGATTTCGATTAGCATATGCGGCTTATAGAGTTGCGGTCTTGTTATTATTGTTGTTTTGCTGCGACATTCACTTCGACGGAGTTGTGAGGCATAAATACTACATTAACATACTATTTGAAGTGACATTCCTCGGTGGAGAGTTCTCTAAGTAAACAATTGAACAACGAAGCTCAAGATCAAGAAATATTTTCATACCTACTTAACAAAAGAGTGGTATTAATGGGGGGGTTTTCAAATGGAAAGACATATGACCTATCTAGAAAATGAAGATATGAATTTTATACTTCAAAAATAAATTCGAAAATGATATATGTAATATGTAATATGTAATGTAATAGCACATGCGTTAATTAAATTATCGGTAAGAAAATCTAAGGTAAGGAAAGCAACTAAGTCTGTACTCCACTGATGTCATGTTCAATAGATACTTCTATATGAGACGAACTTTTCTAGCAATCAAATGCTTTCAATGAGATTTTCTCATAGTGAGGCTCTTCCAGCAATCACATTCCATTTCGCAGAAGACAAGGCGTTTAATTAAATTGAAATCAACAGCAATTTAGCTGCCAAAAGAGCAGACAGTCCATAGAGCAAACAAAAAAGAAAAAAAAATGAGGAAAACTGATTAACACATTGCTGCGTACATGGAGCATAATGTGTGTATGTGAAAAAGTGGCTTTACACGCACTGAAAAGCTAACGATTTTTGACTGCATCGCATATGTGTATATATCTGGTGGGGTATATAGCGAGTTTTGGTTACGAGTCGGGCTAGCCTGCTACCGTTTAATGACGACAGCAACGAGTTTTGGCTTTTATTCGTTTCTTTATGCCCGTGCTTTGACGCGATTTATGTCATATCTTATCAATTACAACTGATTAGAGCGACGGGTG

TCATGAGAGACACCAGCCCATATATATGTAACCCATTCCATTCCATTCGATTCGATTCGATTCGATTCGTTTTGATTCCCCCCTTTATCAGCGAGGTCTGAACTAGTTCATGGACAATTTGTAGTCTGTGAGGCGATCTGTGTTGTTCCCTACTCTGACATTAATCAAATGCGATTAGCTGGCTTCAATAAAACATAACGATAATGACGTCTCAATTCGAATCTCCCCTCGCATTTCCAGGGATCCATAGAGGAATGGCTGCAGCTGCTGCGTCTGGAGGAGTATATACAGCCCCTGCTGGAGCAGAACTATAAGACGGTGCGCGACGTAACCCAAGTGACTTGGGAGGATCTCGAGGACATTGGCATTGTCAAGCTGGGCCACCAAAAGAAAATTCTGCTGGCCATCAAGCGTGTGAAGGACATAATTAGCGGAAAGTGGAACCCAGGCGGCGCCAATGCCTACCAACAGCAGGTGAGAAGTGGGTGAAAATACAAGGGATTTAGGAAGAAACAACAAGGGTTTTTTAAATTTTACATTACTGCAAACTTCAATCTAAAGCATTTGTTGTATTTTTTCTTTAGTCTTAATTTAATTTGATTTTAATTTACCACTAACTTTCGTTTCGTTTCTATTTTGTTCATTTGTTTTGTATGATTTCCCCTCGTCGGAATGTTGACCAAACAGGAGTACCAGCGGAAGCCCTGGCAATTCAATGTGCCCATACCCAGTACGCCATCC

 

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