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CRIMIC CR00677

Gene Drep4

CG9414

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:13286105..13286113	34B4	FBti0200631	80624

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	OK	CTTATGTCTTTGCTCAACCTCAGGCG	TTGCAACAAGTGCGGGAAGGATTGTG

Images

Construct Design Sequences

sgRNA sequence	AACTTTCAGCCCATCTACGTGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AGTCGGAGCACAAGGAAGTG	ATGGGCTGAAAGTTCCTGGC	GGTGGGTGTATATTTCGCGC	GACACACTGCGCATTGGTTT

 

Amplicon 5'

Amplicon 3'

AGTCGGAGCACAAGGAAGTGGACCATTCGTCGAGGATGCAGTTTTCCTTGGTGTACTCCCTAATCACCTGCCTCAGTCTCTTGCTGGGTATCCTGCCAGCGTCTGTTTCCTCGTCGCCCAAGTGCTGCTTCAGGAATTCATGCTTTTGCTTGCAATTCCGGTCAAACATCATGGAGAAAAAGTCGCAACCGATGAGCAGGTATCGAAACTTCTCCAGCACAGAGTTTATCACTTGTCGGGCCTTGGCATTTTGGCGGTACAAGGGATTGCGGGTCAGCTCCTCCTTGGCCTTATAGTAGTAGCCTCGCACACGTGTCTGGGCACGTGTGGCCATAGCTTCCTCCTTAGAGTGGAAGCGCTCCTCGCCGCCCGTGAACCACTCCACGTGCTCGGCCTTTAAGCTGAGGTGGGTCTTGCTGTTATCCAGGACGCGTCGCTGCTTTTGGTGTTCATACTCCGTTATCATGCGGCGGAACTTTTCCGGATGCTGTTCGATAAAATCGTAGAAAATGTCAGCCACCTTTAGCAAAGGCGATTGTTGTCGCATCTTCTCAAACTCAAAATCGGCATCTAATAGAAGACAGATTAATTGTGATTAAGTTAAATATTTAAACTGCAGCTCACCTGTTGTAATGACTGCATCCGGGCCAGACACTATGAACAGCGTCTGGGCGGGTAGGCTTTTTAAGTACTCATCGTCGGACACCTCGAAGCCATCAGAAGCCAAATACAAATGTGGCTCGAGAAGCTAAAAGGTAAAAAAAAAACGATTTAATTGTAATGCCCTTGACATGGCCGAATTATAACGAGGCCAGGTCGCCCAATTCCTGGTTCAGGGGCCTTCAGTTTGTTTCACATTTCTCTTTTTTTTTTTTTGCCAGGCAAGGACGTTCCATCAACAGGTGGTTAGCTCTACTATACAGGACATGCGCATTGGCATTTACTAGTGGCATTGAAAGAGAGCCAAACAAACGCGGAAGCCACTGCCAGGGACATGGCCTTGAAAATTACCAGAGCCAGGAACTTTCAGCCCAT

GGTGGGTGTATATTTCGCGCTTTTACGCAGGAGTTTTTCCTGTTTTTTTTTTCTTACCGGGAACTTGCTTTTTGCCTTGGCTATCAGCATTTCCAGGGAATTGGCTCCGATTCCGTATTTCCTCGTTCGCTCGTTGTCAGTAACCTTGTAGCCACGCATTGGAGCACCTGCTGTTCCCCTGGATTCGGATGAGCTCACATCCTTGAGAACTTCGCCTGTTGGCTGGCTGCTTTTCTTGTTATTCGAGCTGCTGCGTGTTGTCGGTGACATGATTGCATCTCTAATGTAGCTGATCATCGTTCTGGCTCATAATAAAGTCAACTTTGGAATGTAGTCCTCGCAGCAAGTTTCCTTATCCGCGGACAAAGCAAAATAAACTAGCAGAACAAGGCATTTTTCGCGCTTCTCAACACTAGATTCTCATTGAAAGATTCTTTAGTTGTGCTGCAATTTTAGCTAAAAATTGCAAAAAGCTATATAATAATAATTTATTTTTTCCCGTGTTTTGTAATTAACGAAATATTTTTTATATTTTTATTAATCAATTCAAATTGATAAAATTTTCATTTATTCTTAAATTAAATAATGTGTAAATTAATAAAATGAAAATAGCCAAATTAATAAAATACAAGTTGAACGGCGCATTTCCCACCACTATTGCCTTCAGTGTTTTGCAGCACTTATGCATCTAAAAACAGCTGTTCGGGTAATCATTTTTTTTGTTAAAAGTTTCTGTGGAAAAACTATAACAAACGTAGTCTTAGTGTACAATGATCTGGGCTACGGGCTCCGCGTAATCCGCAGCTCACCCGGAAAGGCGTTTTACGATGAATCTACGGCCGCGATGCCGCAAGTGGCTGCCCCTAGCCACCCAGCAACTTCGACTGCGGAACCTTAACAGAATTCAGGGATTCAATATTGAGTCCTGGCCGCATGAGAAGTCGCTGGAGCTAGATGATCCGGATGATGTGCTCCTCTACTACACACTACACACGGACAAGGCTAGCGAAGCGTTCTACACCAGCGAAAAGCTGCCGCAGCGTCACCAACAGCAAAAGTGGGCGGAGATTTGTACGGACGACGAGGCCTGGCGGAAAACCAATGCGCAGTGTGTC

 

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