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CRIMIC CR00680

Gene gammaSnap1

CG3988

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:24047398..24047407	60B5	FBti0199444	78982

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTTCCTGATTACGATAGTGCTGCGGA	AGCTGCCACTTTGGAGCATTCTTTCG

Images

Construct Design Sequences

sgRNA sequence	TTGTTGTCGATAGAAGACTTGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AAAAGTGCACAATGACCGGC	GAAGACTTGGGACAGTGCGA	ACAAAGGCAGATTCAATCGCC	TATCAACAGCACGGTTCCCC

 

Amplicon 5'

Amplicon 3'

AAAAGTGCACAATGACCGGCCGCTTTTACCACCGAGTCTGAATGCGAGTCCCAAGTGTACGTACCATTTTTCCTGTAGTTTTTCGCTTTCACAATTCTCAAATTGCTGCTTCGCCGAGTATATATTTTACAAATACTGTTTAACCTTCCGCCTTCTTTCACGAACAACCGGACGAATCAATTTGGTCGGTTATTTCGCACAGGTTAGGGTTGCCAGGCGCCGGCAAAGTATCAACATGCGTCGTGACGCGTCGCGACGTTTGTGAAGCGTTTGGAACGGTGAAATGGCACACTTTCGATCTTTCAAAATGTCAAATATTTTAGGGTGCTTTAAAATACATAGTATAGTTTGAGTTAATTTATTTATTTATATTAACAAATTGCACTATACTTTCGTGTAATAAAGCTGAAGATTTACAGATTTGAATCTTAACACATTCGCTGTTTTGGAACATAGAATATATATACATAGTTTACACTTATAGCTACAATTTATTACTTCAATATCAATTCATCCCAAAGCTAATGATATTCTTTGTTGTATAGTATTGATCTTAGTATTTATTAATTAGTACAACGATTGCGATGAAATTTGTATTTAACAGTTAATATAATATGTTAAAAGCGATATTATTAACGATAAATATATTTTGACTAACAAGCAGACCGAACATTAAGAAATTTAGCACAAGCCACCTTCGTCCTCGGCGTCGTTGGTTTCCTGCGAGAGAGAAAGAGAGAAAATACGAAATTCTTGTTAGCTATTTAGTTTACTGCTTCGCACTGTCCCAAGTCTTC

ACAAAGGCAGATTCAATCGCCTATCGATAACATCATAATAAGAGCCAAATTTACAAAACAAAAATTGCAAATTCCATTTTTAGAATAAAAATACTCACAGCAGCAGTGTCGCCAGCCTTCTTCTCCATCTGTATACCCTGAGGTAGTGGAATGTTTTTAGATAGAATAGCGTACTCAACATCCATATGTCGTATGAATGGGGATGCCAGCATCCTAGCAGCTAACTCGGGATCCTCGTCATCGAAGGCTTGCAGAAGGGTCTGCAGTGTGGACACTTCCTCTGGCTCGCAGCAGTTTCCCCACTCCCTGAAGGTCTTTTCGGCTTCCACGGAATCCCCGCGAGCCAGTTGGACCATCACCAAGGCCACAACTAGGCGTCCAATTTGTCCGTAAGATTCCGTTTGCTGATTCAAGCTGATCTCCTTTTTGAGCGCATTCGTGGCTTCGTCGTACCTGGAGTGATTGGATGATATAAGCTAAGGATACCCAAAGTGATTCGTTGCAATCATAGGTACATACCTCCTTAGTTTGACCAGTATCCTGGAAACTTTTGATGCATACTCGGCTGCTTGACGGACGGAATCCTCGATCTGTCGGGTAAGTATAAAATTAATAGTTTAAGAATGGAATTGACTAGAGCAGTGTCAAATAGGACGAACCATTATGACTTCTAGAGCATGCTGATAGAAGCGCAAAGCCATGTCAGGATGCTTGGATTCAGTTAACTTGGCGGCTTTATCCAAGGCGGATGCGGCTGCCTCTGGGGAACCGTGCTGTTGATA

 

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