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CRIMIC CR00683

Gene ncm

CG12750

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:18448223..18448245	36F5	FBti0199445	78983

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCCTTGTTTAGTGTAGGCCGTCGTGA	TCTAGTGCCAGAATCTCATGTGCCAC

Images

Construct Design Sequences

sgRNA sequence	CATCATGTTTGGGTAGAATTCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GTTTCGGTGGTTTCGGTGTG	TCGGTATTAAGCCGCTTTACT	TTACGTCGGTTATCTCGCCC	AACACGATTGCCCGTTTTGG

 

Amplicon 5'

Amplicon 3'

GTTTCGGTGGTTTCGGTGTGGCGTGGGGTCTTTGAGGATTTGGATTTCGGAGTGGAGGAGGATCGATCTCTTTTGCGTTCTGGCGACCTCTTCTTTTCCCTCGAATCATCTCTACGCCTGCTGCTGGTTGGTTCGCCCTTTTGATGGCGCTTTTCTCGCTCCTTGGCTCGCTCTTCATCGCGTTTATCTCGCTCCTCATGACGTCTCTGTCGCTCAACGCGTCTTCTCTCCAAAGAATTGGATCTGCGGCGGATTGGACTTTTACTGCGACTCCGGGTCCTGCTGCCTTTGCTTCTCGAGCGACGACGACGACGGCTGCTGGGTGATGGACTCCTGCTGGATGAGCGTGGGCGTCGTCGTTTGCCATCCGGGCTGCGGCTTTGTTTTCGTTGGGTTTCTTTGGGTGGACTCTTAGATGCGACAGCTTTCTTTTCCTCTTTAGTCGGTAGGTCATCTTCGTCTTTCTAAAAAATGAAAATTTATCATATCATGATGATGAAAAAACTAGCTGATAAATGATTGTGTTGAATTAATTTCATTAATTAAATAATTTGAATTTCATTGATGACGAAATAGTAAAGCGGCTTAATACCGA

TTACGTCGGTTATCTCGCCCTCTTCGATGTGGTCCGCAGTGGGAGGTTTCACTGTTTCAGCTGCTGCTTCCACCGGCGATTCCTTGCTACTTCCATTGGTGGTCACTGGCTGCTCTTCATCCTTCCTCCGCTCTTCTAATTCCCGTGCAGCGTTTTCCTCTTGGGCCTTCGGGCTGTTCAGCTCTTTCGTCACACTTGAGGCTTCTGCTTCCTCTTTCCGCTGGACAGGTGTTTCAACTTTTTTCGCTTCATTTAATTTCTCGCTTTCCGCCTCCGCAGCTGCTACTGCTTCTTCTTGCTGCTTTTCCACGCCAGCAGAATGGGAAATGTGCTGGCGCTCCGAACGAGGCTCAGCCGCAGGAGTTTTCTTCTGCTCAGTGGGCTGCTCATCCTGTCCAGCATCCCTCTCCTTGGCACGCTTCTCCCCCCTATCCGTATCGTCAGTTTTCTTGGCATCTTTGGCGGATTCCTCCTGCTTTGCTTCCTCTTCCTCGCGCCCGCTGCTTTCGGAGGACGAGGACTCGCTCCTGGCGTCGCTGTCGCTGCCACTGGAAGTGTCGCTGCTGCTGGAATTGCTGCTCGAATCGCTCTCGGCATCGCTTTCCCCCATCGCGCGTTTATTTCGAGAAATTTTCAGGGAAAACAACGATGAAAATTATTGGGCAGCAAAAAACACAACGCGGCGATAGAATTAGAGATGGGACACCAGTGGCGACGGTATCGATATACACATGTGTTTGACTGCTAAATCGTATCGAAATTGTAGTTTCGATAGTCAGCTATAGAAATGCTAAATGTGTTTTTTAAATTGAACTGAAAATTTAAATTCAAGTTCCACATTAGAAAAAATGATATTTTAAAATTAATTTTATAATATGGCACTATCGTAAATTTTATAGCACAGCGAACCTCAATATTTAAGGGCAACGATTTTTTTACGACTAAAACAGTGTAATATATTTGTTATAACATATTTGGTTCATTGCACTGCAGAAATTTCGAATATCAGCTCGAAAATGGAACAAGGATATGGAACAAGAACATTAAATTTGAAACGAAACTTTTTTTTTTTAGCGATTTTTGGGCTCCAAAACGGGCAATCGTGTT

 

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