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CRIMIC CR00684

Gene boca

CG30498

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:7566499..7566512	43D7	FBti0199446	78984

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCGAAGTCTGAATGCTCCTCCGTCTT	TCTTGTTGCGCAAGCTCTTGACCATG

Images

Construct Design Sequences

sgRNA sequence	GTAGGTAACAACACTGCGATTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCTAGGTTGGAGAGATCCGC	CTGCGATTGGAAAGCGACAC	CCTCCCATTGATCTAGCAGACG	TGATGTGGTCGTGGTGAGTG

 

Amplicon 5'

Amplicon 3'

GCTAGGTTGGAGAGATCCGCATTGAGAATGCTGGGACCGATCTTGGCTTGGACGGGCATCTTCTTGGTGGAACAGCTACTCAACAGGCGGAGCAAGTAGAGCACGTCGCGGTTCGCGGGTGGCGTCTTGTTGACTTAAGGTCACACAGAGGGGAGCTGGAGCTCGAAACATTTGAAAGGCGATACGATAACTTTGCCGATGGCACCGTGAGTTGTGTAGTTGGTCAGCGATTAATTTAGAGTTTTGCTTAAATTTTTCATTTATTTTGTTTTTGATTTGACACATACGCAGTGTATATAAACAATCTAATAGAGATACAAATATTATATTCAAAATATGTTTATTGAAATATTCATATTTTTATCCATATTTATTCCATCCCGCAAACGGAGCCGTTTCCCTGTGAATGGCCAAGCAAAATACATATGTAGCTTCTTTAATAAGTTTGTTTAATAAATTTGTTTTATTTTAATTGAACTTTGATCTTAAATAGTGAGAATGTTTTGATGTGCTGGTATGCCGTGGAACCGGCCTACAGTTCGTCCTTTTTCGCATTGACACCTGGGTACTCCTTGTTCTCGATGGTTACGCCCTTGCAGCGTTCCTGCTCGATGAGGAAGTCCTTGGCGTCCCAGGCTTGTGTGCCGTCCTTGAAGAGGAAGATGGCGCGGTTATCGTCAACCATGTAGCGCTCCGCCTGGATGTGGTTGTTCCAGAGGCTTGTCTGCCAGAGCTTGGTGATGGTGTCGCTCTCCTCCCGGGTTGGATTACCGGTCACCGAAACGAAGGTCATCAGCGTGCGCCCCTTTTTGGACACCTTGAGCAGGTCCTCGGGGCTCTTGCTGTCCAGATTGCTCAGGTCCAGCTTGGGCTGGGGGCGCAGGTGCTCGGGCAGCTCATCATCCTCGAGGGGCTCTTCATCTTCCTAAGAGAAGATTAAAAGAAGGGGTATCAGTTATCCGTCTCTACAAATGATGAATCCTTAGATAGGTGGGAATCTAAAGGGTTGGCATTACTCCCTTGCTAGGTGCGAATCTGGAGAGTTGGGTCCGATGTGTCGCTTTCCAATCGCAG

CCTCCCATTGATCTAGCAGACGCTCGAGGTCCGCCTCGCTGTAGTCGCGTATATCCTTTTTGGCCCACGCCGGTTTCTCCTCCTCCTTGAACTTCTTGGCCAGGACGAGCGGGGTCAGAGCTAGCAGCAGCAAAACAAGACGTGTTTGCATGGTGTTTTATAAATATTTTTCCTATAGCTCAGCAATTCGCACGGTCAAACACGTCACCTAACAGTTTGCCATTCGCCCGGGAACGCGACCGAACAGCTGATTGAGCGTCCCCAGGTGGAGAACGCAAACGGAAAACACAACAGCTGTTAGTTTCGGGCGAGCATTTAAAGAACGCGCATCGCACGGAGTTACTACTTTTTTGTCGCGTGGAAATGCCGCTGGTAATTGGGTGCAGTAAATAGAGATAAGAACGGGCGTTGCACACGTGCTCTGGAGTGGAGTAGTGAGTTAATCGCACCAGCCCTACTCCAGCTGACCCTGGACAATCGTTTGGCTTGTTAGTTTATTTTGGAACAATGGGCCTGGCGGATTGTGCCAAAATATTTGAAATGCGTTCAACGATGACATCATCGACATTGTGTTTTAATCCGTCTCTCCGTTCCATCTGTTTGGTTTCTGTGTTTACATATGCATGTATTTTAGATCAACTATAGACCGCACAGCGAACGCCAGATTGTTTGAAGCACTCACCACGACCACATCA

 

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