• About
• Search
• MiMIC
  • Technique
  • Constructs
  • RMCE Lines
• Transposons
  • GDP
  • Others
• CRIMIC
  • Technique
  • Constructs
• Order
• Downloads

CRIMIC CR00685

Gene Gprk1

CG40129

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:4431133..4431145	41B3-41C2	FBti0200633	80625

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	GTACATGAAGGCGGAATCTTTGTCGG	TCTTATCGGTCTGCCTCCAGTTGGTT

Images

Construct Design Sequences

sgRNA sequence	CGCCTAGTTAACGTCATACGCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TTGTGTGGCTTGCGACAATG	ACGCGGATTAACAGTAGCGT	TAGGCGTCTTGGTACAGGGA	CCGCTGAAGGGTGTGTTGTA

 

Amplicon 5'

Amplicon 3'

TTGTGTGGCTTGCGACAATGTTCGGCCGTGTGGTCAAACTTCTGACATCGATGTCATTGTACTACATCATTTTTCCATTTTTGCGAGCAGTACTTATGGGCCTGCGTGCAGCTGCAGTTCATGAAGCTCCGAATAATAGACAGACTTACAGGATGGTGTTTGGGCAAAATCATTTGGTGTCTAGCATCGTATGAATGTGAAGAACTTTCCACACGGCCGCCAACGCGTAAAGTGTCATTCTCATCCAGAAAGGGCAGTAGCCACGCAATACAGCTCGAGTTTTTGACAATTCCCTTAGAATGCAACTCTTTAATTTTGTCGTTCGTTTTACTTCTGTGTGCAATATCGGAAACCCAAAGCCAATGATAGCGATTCACTCAGTAATCGATATTACGCCTCTAAAGAATTCGGGCGGTGAGCGTGGAAGCGGGTAATGAGGGTCAGGCGGTAGCAGAAGATGGATATGAGACGATGAGGCTGAGATCAGGTGAGACGAGAAGACTGCGATCAGGAAGGTCCAGGACAACGGAGGGAGGCTAGAGAAAGGCGGAAGGATGTGCGAGATGCCAAGTCAAGACGCTAAACGACAGGGTCTACTAGGCTGAGACTGAGAACGAGCGAGGGTCTAGGGTCTACCTTTGTGAAACCACCACACTTAAAAAAAAAACCCACACAAATACATAGGTTAATTGCGAAAGGAACATTGAGGTCCAGTAAGACGCCTGGCTACACAACTAATTGATACCAGACACTGGCGTTAGCCCGGCGAGTCCGTTAATGTATGAACAGGCGTGCCCAATCCTAGCAAAGACCCGTTCCCGTGGCGGGATTGTGGTAAAGATCCGGTCGACGGTTTCAGTGTTTGTGTACCGTAAACCAGACGATTGCCTTTAGCATGCGTAAGAATGAAAGACGCTACTGTTAATCCGCGT

TAGGCGTCTTGGTACAGGGACTGTGGCGTAAACCGGCGGATTCCACGGAGCATCTGTAAGAACTGAGCAGGGGTCTGGCGGGACCGGTCCGAAACCAGTGATTTCAAGACAACAAACCAAATACTCCACTTACCAAGCTACGACGACATACTCTGGCAAACCTGAACACCCCCAACAAGCTACTACAGACGATGACATGGACACTACCGCAGCAATTTCACAAATACATAACAAAAAGAACATGATTGATTCGGCGCGTAAGCTCAAATCCCATACAAAAGACAAATCAAGCGTACTTAGTAACCCACTTGAATCTTGAAAGACAAAACAGAAAAATCAATTATCCCCATCAGCAATCTCATCAACAAACTACCACAAACAAACCCCGTACAGTACTCGTACTGAGGCTTAATAGAAGGAAACCGAGCGACCTCAATCTTCGATCTCCTTCAAAAAGGGTCAGTAGACACTGTCATTTATACAACTTTTATCTGTTATTGTCCCTTTTTGTTATTGTTGTAATTTATAAATTTCCCATCTGCATATAATCATCATCCATGGCAACCGAACAAGTCAAACTGTTATCGGAATCCCATCTGAAACAAGCCTTAATCCAAATTAGACAAATACCAACATTCAGTGGTAATAGCCAAGAAGTCAGTGCTTTTTTCCGAAGGATCGAGTTCATACTTCAGCTATATCCATCAACCGACACCAGCATGTATTCTTCAGCGCAATTGAGATGCAACTTGCAGGAGATGCTCAACGAGTATCCCAATTATCCAGAGCAACAACCTGGCCAACCCTTATGAATGCGCTCATCAGCGAATATAAAACCCAAACACCATGTGTAGAACTACTCTGTCGTCTCTACAACACACCCTTCAGCGG

 

---

Search Again