CR00688

CRIMIC CR00688

Gene Chd1

CG3733

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:2986439..2986439	23C4	FBti0199447	78985

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCATGCAAGTGAGCCCCCTGTATTTA	CTTTCATCGCTTTCATCCGAGTCCCA

Images

Construct Design Sequences

sgRNA sequence	TCTAAAGTGCGACCTCACTCTGG
sgRNA2 sequence

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CTGCTGCTTGTGTTGGCATT	AAAGTGCGACCTCACTCTGG	AGGTCGCACTTTAGATTCCACA	ACACTTTGATGACTGGCCGT

Amplicon 5'	Amplicon 3'
CTGCTGCTTGTGTTGGCATTGTTGATGCTCTGGTTCCTTTGATCCGATGGGCCCTCGGCGTCTGAATCGGAATCACTGCCACTAGACCCGTCACTGGAGCTGTCCTCCTGGTCATCCGTGAACCCGTTCGTCTTACTGTCCGCCTGGGCAGCAGCGGCTGTCGGTGGAAACCCTGCCACCGATAACGATTTGTCCTCGGGTTCTGGGGAGCTCCGTCCATCGCTAGAGTTGCCCGAGGAGCTGTCTGAATCTGAGTCTGAGCCGGAGGAGCCACTACCGCTGCCACTGCCACTATGGTCGGTGCCATTGGCCTCTTCCCGAGTGTCATCTTGTTCGTCTGAGCCAATACTATTCGCCGATTCATTGAGTGCCTGGTCAGATGAAAAGTAATTTCAAAATCAATCTAGGTTGAAGATCAATCAATCTTAGTTAAAGATAAACCGTCGGATACGAAAAGGTTGAAGTAACACACCGGCTCTGGGCCAGCCAATCCTGGCCAGAAATTGTGAGTACAGAGTGCATCGAAATTGGCATAACATGTTTAATATTCCCCTTATTCAGCTCCTTAGAAGTAAGGAATGGGTTACACAATCACTGAACTATAGGCAACGACTTAAAGTGACATTTTAAAACAAGAAGATGGCTCAATCCTGTCCAGAGTGAGGTCGCACTTT	AGGTCGCACTTTAGATTCCACAAAAGCCTGGTAAAACCACGGAAAACTTCGAAAACTTGGCTCTTGGGCCGTGCTGAACAGATTATAAAACAAAACGGCGTGAAGAAACTTTCATAAAACGCACTCTCATGGCTTACACAGGACTAATCCTCTCCAGAAATTTGTTTATGGCAGTTTTTGGCCAAAAAGGGTAAAACAAAATGTATTGCGTTTTGTAGTTCCAGGAAGCACACGACAGAAGCACATGACTACTTTGAAGAGAACGCGGTAATCACACGACACTTCTTCCTGGTGATTAGAAACCCGGATATCCTTAGAAACAAGCGGGAAATCGATGTTCTGGGCGGGATCTTTCCCTGAAAAAGGCACAAAACTGTGGAAATAGGAATGGCAAAGCGGAAAAAATTATTTTTAATCATTGTATCTGTCGAAAAGAGGAGGAATTTAAGGAAAATTATTGCTGGTAGCCTTTTGGCGGGCTCGAAAATCCCTGGAATTTGGTCAGAAGTACAAGAGAGGAAACTTTGCACCTAGCCATTTCTGATTACGTAATCGGTGAAAATTTTCCATTTTATCGAAAAAGGAAGGAAATCCATGGAAATTACAGGATTTTAACAGCCGCTATTGATAAAAACAAACAGAAATTCTTGAATTTGAGTAGAATTTGCGAAAATGGGTACCCAATGGATTTCCACAAAATCCACCTTGGCTGCCACGTTGAAGCCTTGGGAACTGTCAGGCACTGTCGGAAGGATGAAACACTCTCGGAATAGTGGAGAAATAAATCCTTTTATCCAAATGCTCCGCCAGCAGCCGCACGGCCAGTCATCAAAGTGT

Amplicon 5'

Amplicon 3'

CTGCTGCTTGTGTTGGCATTGTTGATGCTCTGGTTCCTTTGATCCGATGGGCCCTCGGCGTCTGAATCGGAATCACTGCCACTAGACCCGTCACTGGAGCTGTCCTCCTGGTCATCCGTGAACCCGTTCGTCTTACTGTCCGCCTGGGCAGCAGCGGCTGTCGGTGGAAACCCTGCCACCGATAACGATTTGTCCTCGGGTTCTGGGGAGCTCCGTCCATCGCTAGAGTTGCCCGAGGAGCTGTCTGAATCTGAGTCTGAGCCGGAGGAGCCACTACCGCTGCCACTGCCACTATGGTCGGTGCCATTGGCCTCTTCCCGAGTGTCATCTTGTTCGTCTGAGCCAATACTATTCGCCGATTCATTGAGTGCCTGGTCAGATGAAAAGTAATTTCAAAATCAATCTAGGTTGAAGATCAATCAATCTTAGTTAAAGATAAACCGTCGGATACGAAAAGGTTGAAGTAACACACCGGCTCTGGGCCAGCCAATCCTGGCCAGAAATTGTGAGTACAGAGTGCATCGAAATTGGCATAACATGTTTAATATTCCCCTTATTCAGCTCCTTAGAAGTAAGGAATGGGTTACACAATCACTGAACTATAGGCAACGACTTAAAGTGACATTTTAAAACAAGAAGATGGCTCAATCCTGTCCAGAGTGAGGTCGCACTTT

AGGTCGCACTTTAGATTCCACAAAAGCCTGGTAAAACCACGGAAAACTTCGAAAACTTGGCTCTTGGGCCGTGCTGAACAGATTATAAAACAAAACGGCGTGAAGAAACTTTCATAAAACGCACTCTCATGGCTTACACAGGACTAATCCTCTCCAGAAATTTGTTTATGGCAGTTTTTGGCCAAAAAGGGTAAAACAAAATGTATTGCGTTTTGTAGTTCCAGGAAGCACACGACAGAAGCACATGACTACTTTGAAGAGAACGCGGTAATCACACGACACTTCTTCCTGGTGATTAGAAACCCGGATATCCTTAGAAACAAGCGGGAAATCGATGTTCTGGGCGGGATCTTTCCCTGAAAAAGGCACAAAACTGTGGAAATAGGAATGGCAAAGCGGAAAAAATTATTTTTAATCATTGTATCTGTCGAAAAGAGGAGGAATTTAAGGAAAATTATTGCTGGTAGCCTTTTGGCGGGCTCGAAAATCCCTGGAATTTGGTCAGAAGTACAAGAGAGGAAACTTTGCACCTAGCCATTTCTGATTACGTAATCGGTGAAAATTTTCCATTTTATCGAAAAAGGAAGGAAATCCATGGAAATTACAGGATTTTAACAGCCGCTATTGATAAAAACAAACAGAAATTCTTGAATTTGAGTAGAATTTGCGAAAATGGGTACCCAATGGATTTCCACAAAATCCACCTTGGCTGCCACGTTGAAGCCTTGGGAACTGTCAGGCACTGTCGGAAGGATGAAACACTCTCGGAATAGTGGAGAAATAAATCCTTTTATCCAAATGCTCCGCCAGCAGCCGCACGGCCAGTCATCAAAGTGT

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