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CRIMIC CR00691

Gene Sox14

CG3090

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:23983009..23983009	60A16	FBti0199449	78987

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATATAGAACTGGCAACTGGGAGCTGG	CAATGTTGTCTTCGGAGTCGAAGACG

Images

Construct Design Sequences

sgRNA sequence	CGCTCATACCGCCTTGTCTATGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CGGCGACATTGAGAGTGAGT	TTTCGCTCATACCGCCTTGT	ACAAGGCGGTATGAGCGAAA	TCAGCTCAGAGTGCAGCTTC

 

Amplicon 5'

Amplicon 3'

CGGCGACATTGAGAGTGAGTTTCGGAGCAGAGTTGCGAGGGCAAAGGCAACAGAACGGCAGTGGCAGTGGCAGTGGCAATGGCTGAGCCAATGGCCGATGGAGCATCGCATCCCGTCGGCCCCAATACCACGTCTGCTCCACTCAGCCCGCTCAGCGTTCGGTTCGGTTCAGCTCGGAGCGCCAAGGGTATCGCCTCTGCTTCGGATGTGGATGAACTCGCGAGGTGCACGAACTGCTGCAAGGTCGAAGTTAACGCTATTAATAGGTGGTTTCTGCCGTCTCTCGCGGCGCGAATAAAACGGTTATTCACATTGGCCAAACGAGGCGGCGCGTCACCGCAATGACCTATTCCAAGTCACCAAGTCAGTCTTCCTGGCCTCGCGAGCCCAGTTTCCAGATGAGTGCCCACCTGAGATCGGGCTGGATCGGATGTTTGGATTTTTGGGATGGTCAGGATGCTTAGGGATAAGTTGGGACCAGAAGAACGGGGCAACCAGGAAGCGGACCCACTTGTCCGAGCATCGCAGCAGCCGCAACCCGTCGAGCAGAAACACATTTCAGTACAAATGCACACAGACACAAGCCATATTTCGTTTTAGGAGCGTTTTATTAACCTTGGCGAGGGACATTGAACTTCAAACGCGGCGGATACGTATTTCGCACAGATACAAACGCCATAGACAAGGCGGTATGAGCGAAA

ACAAGGCGGTATGAGCGAAAATGAGCGATGCATGTATGTCGGGCACACAGCCGAACTGTTCAGATGTACTATATACGCATATATGTATATATACTATATCTGGCGTGAATTTGTTTCCTAACTCTTTATCTGCGATACGTTAATATGCAGTCGGCATTTATATATATTATTTATATGCGCAGGTACAATAGGTAAATGTGTTTTTCGCATGAACGAATTAACGGAGCGTATCTGCGGTTGCCGAAAGGTAAAGCAAATTTCGTTGTTCCATAAGTTGGTGGAAACAAGGGAGTGCATTAATTTACGGGCTCTGAAATGCCGGAAGTGAATTTAATATCGTACGGGCGGCCACATATGTATTACTCACTCACTTTCCTTGCGTTGCACTTGACGCCTTGCCTAGGCCAACATCTGTGGGCTTAGCGCTGTGTGGGGATCCCGCCCCCACCCACTCACAATCGCATCTAAGATAGTTATAACTAGCTATAAGATTGAGGCAATAACTAAACCGCCTTCTTTTCCTTCCACCTGCAGACCAAGAAACATTCGCCCGGCCATATCAAGCGACCCATGAACGCCTTCATGGTTTGGAGCCAGATGGAGCGGCGCAAGATCTGCGAGCGGACCCCCGACCTGCACAACGCCGAGATCTCCAAGGAGCTGGGCCGCCGCTGGCAGCTGCTCAGCAAGGACGATAAGCAGCCGTACATAATCGAGGCGGAGAAGCTGCGCAAGCTGCACATGATCGAGTATCCGAACTACAAGTACAGGCCGCAAAAGAAGCAGACGCGCTCCCCGGGATCGCTGAAGCCCAACCAGGACGCGGACGGCTGCGAAGCCAGAAATGACACGACCAACAACAACAACTCACTGACCACACTTGCAATTAATGGAACCACCACTGCCGGCCGCAAAAGCAAAAGATCTACCTCTACATGTCAATCGGGTTCCGCTTCGAAACGATTGAGGAACGACTCGGGTGATACCTCCTCCAAGCCTAAGTACGAAGTTAAGATGGAGTCTGCTGAGCAGCTCAACTCCGCCGATATTATACTACCCTCAGCCGATAATCTGATAAGCTACCAATCGTCTGAATACTTACCTCTAAGCACGCTCAGCAACGCCGACTGCGACGAGAAGCTGCACTCTGAGCTGA

 

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