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CRIMIC CR00693

Gene Nox

CG34399

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:16310895..16310897	53B3	FBti0199450	78988

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGCCAGAGCGAGCTGATATCAGAGAT	CGCATTCAATTGCCTCCTTCCACTGA

Images

Construct Design Sequences

sgRNA sequence	ATTACGTGGGTCAGCCACTGGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GTCTCGGAAAGTGACGCTCT	TGGCTGACCCACGTAATGA	TGGGGTAAACACTGTGCTCC	GAGGCACGCGGACTATGTTA

 

Amplicon 5'

Amplicon 3'

GTCTCGGAAAGTGACGCTCTGGAGCGCCCTGACCTTGTGCCACTTATTCTGTGGTCCTTTACCCGATCGCTGGTCGATCGCAAAACCCGTTTTTGGGTTGCTTGATGGGCTCAGCTTTTGCTGAGCTGGTGTTTATTTGGTTTCGGCTCCGTACGATCGACCACGAGCATTTTGTTTTTATGGGCTCCCCGAAAGCCGTTTAACCAGTCAGGGCTCATCCGTTAAATATGCACATTTTTCATCATCGCCGGGGGATGGAAGATGGGAGATCGCCACAACAACTGTGACCAAATGCAAAAGACCCAGCGCTGCGCTTCGATTTGCAGACAATGCTGGGCGCTCATGGATCGTGAGCTCGAATGCCATTAAGATATTAATAAGCGTCTAATTTATGCCATTCGTCTTCATCACTACCGCATAGTTCGATTCCCATAGTTCGAATCACCCCATAGTTCCCAGTCGTGGGACCAGAACACTCTTGATTATGCTCATTACGTGGGTCAGCCA

TGGGGTAAACACTGTGCTCCGTGCACATATATAAATCTTTGGTTGCGGGGCATAAACAAATGCTTCTACAAACAATACGAACGTAAATTAATTAACACTTGCCCAGAAACGGGAGACGCATAAATCCCAGCGGTTTACAACTTGTTTTTCGGTCTTGCTTACAGGAATTCCAGCAGAAGCTCTTCCAGCTGTTTGATCTCGAGCGGAATGGCTATCTCCTGCAAGATCGCTGGATCGAGCACCTCAAGGGTCGGCTAACGTAAGTGTGGGTTGGTTTGCTGGCGAAGGTGGAGTTGCTTCCTTGGTATCCTCCTGCTCCTCAGAGATCGCTGCTGATGATGTTGCGACGGTGCCAAAACAATTAGCTCGCCTTCGGAAAGGCAAAGAAATAAAGAAAAAATAGCCATACGATCGAGTCCGATCCACACACGCACCTGGCTGGCGTGCTCCAGGCATTCCATTCCAGCTGCGACGACCAGAAGCCCCATGCCACATCACATCGCAGCCCCAGATGCCAGATGCCAGCTCCGTTCCCAGACGATGCCGATGTCCGTTGCCCATGGCGATGCTATCTGCGCCGTGTGTTAACATATACAACAGCTAACATAGTCCGCGTGCCTC

 

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