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CRIMIC CR00697

Gene retn

CG5403

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:23646546..23646550	59F5	FBti0199452	78990

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGTATCTGCATCTGTAGCGGTATCCG	TCCCAATACCAGTTAATGTCCACCCG

Images

Construct Design Sequences

sgRNA sequence	ACTGCTCCTATAGCTCTTGTGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ATGCGGCAGGACAACACTTA	CTATAGGAGCAGTGCCAACA	TTGTGGGCGTCGAGAATCAT	CCTAACCCTCCAATCCTGCC

 

Amplicon 5'

Amplicon 3'

ATGCGGCAGGACAACACTTAGGGACCAAAGGCCGTCCGTGCAACGGGATCATGTCCATATAAATTTTAATGTCCACTGGAACCTTTTTGCATCCATATGCTAGGGCAACTATATCTGTATCTGATAGTTATAAATTACACCCACACCCACACACACACACACACGGGGGCAGGCAAAGGGAATTGATCTGGCGAAATCCAGAATTCCCAGAATCAAGGATTCAAATTTCAAGCACCCAGGCAGCGGATTTGTCGGGTACTAAGTCTAGGCGACAAATAAGTGAGATTATTTTTATAACCCGAACCCAGAACCCCGAATCCCAATTCCAGATTCCCAGTTCACGATTCCAGATCCCGAAGAAACATGTGTGTGCTCAGGCAGCGGCAGCAGCATCTTCGATAGTTATAAAAACGATTATGATTGGTCTACGGCAAACGTGGCAGGGGATAGAATTCTTGTTTGCCGGTTTTATAACTTTATTGCTACGGATTTGGGAGAGATATTGGTAGCGCTTAACGGTTTTGCGGCTGCTGTGATGCAAGTTCGGGGATCGAAATTCGGGAAAGATATTGGTAAATGAGGCACAGGCAGGTTTGCAACCCTTGTGCGTGAAAGCGAGACTTCATTTTATGATTATTAATACGAACGAGTACCCGAGTACCGCAGACAGGCAGGCAATAATGTAGGTACACAGATACAACACATGTGTGTGTGCGTGTGTGTGTGCGGCGGCAGCGACGCAGGCAGCATTTCGATCCAGACTCCTGCCACAAATTTAGTTGATAAACGACCTTTGATGGCGTTGCATCGTCGCGTTGATCAATGAAATAAATCGAAGACTGCAAGTGAAATATAGTTCCGAAATCGGGAAATCATCTCAACAACAAGTGTGAAACGAAAGCATCAGCTCAAAATGCTCATCTCCGAAGATCAAATTCAGGAGGTACATCCACGTGTATAATGTACATATAAGGTGTAATCAGTGAAAAGCAACGAATCCTTCGAGGAACCAATCCAGTTGAATTTCAATTAATTTATTTTGTGTTTGGCTCAACCCCGAGCGGTATAAAGCCACATATGTATATATGAAGGTTTTTTTTTTATTTTGTTGGCACTGCTCCTATAG

TTGTGGGCGTCGAGAATCATAAAAAATATCCCCAGCCCAGCCAGTTTGGAAAGAAGTTGGCAGAAGGATTGGAGTCCAAAGTGCCCGGCCAAAGTCGGGGCTCCAAGTCTGACTTGGTTTTAAATCATTTATGCGAGGAGGTGGAGAGGCGAAAGGAGATGGAGAAGGAGGCAGTTCATCATTCGCATTGACTTAGAATGTGCACCACGGATGGTGCAGGGCTCCGTCTGTAGCTCCGTCTCCATCTCCATCTCCATCTCCAGCTTCGTTTCTTTCTTTTTGCAGTAGGCGGAAAGTATGATGAATTCCGAATTTGATGAGTTCACTCCGATGTTGTCATTAATTCTGAACATACTCGTACCAAGTTGTGACTAGGGTTTTTCCTTCCACGGACTTGGTTCCTGGTGGCAAAGGTCAAAGCATGAGCCGCAGGAAAAACGCAGCACATAGCGACGATAAATAATAATGTATAAATGGCAGATACATTTATGTACTTCCCTCGTTTGTATCTCCGGCTCTATCTTTATCACCTAATTATCCGTTGTGCCACTCGCGCGCCCATTGAGCTCTTCACACTCTAACAAAAGTCCAAAGAGGCAGGATTGGAGGGTTAGG

 

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