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CRIMIC CR00698

Gene Patronin

CG33130

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:17534270..17534275	54C1	FBti0199453	78991

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCGGCGGTTCCCCAAAGAAAATTGGA	GCTGCAATGCATTGACTTCTCCATGC

Images

Construct Design Sequences

sgRNA sequence	CTTTCTAGGGAGTTGTCTGGGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CGGAAAAACTCTGGCTGCAC	AACTCCCTAGAAAGTCGCGC	GGGGGAAAAGTCGGTGTACA	TGAACACTGCGATGGGTTGA

 

Amplicon 5'

Amplicon 3'

CGGAAAAACTCTGGCTGCACTTTGCATTGACTCGCGGGGGAAAAATGTCTAAAACTCAAATTTCAAAAGACCGTGCAGGCAATTCGAAGAAAAACAATAAATGAGTGCTGCTTTGGACGCCCACTATGAATAAACGAACGATGGATGGATGGATGGATAGCATATTTGTATTCAAGCATCCAAATACACACGAAAAAAAAACAATCTAATTTAATGAGAATTAGGTTGCTGAGGGAGTAATAATTAGAAAGCTAATAAACTAGTGTTTTCTGAGATAATTGTTATATTTAAGAAAATAAAACTAAAAATGAATATAATGATGATTTATTGTTGTTCAGTTTAGAGATGTTTTTTCTCTGTGCCGCAAACATTTCCATCACATGTTTGTAATAAAGTTTCAGTTGCTGGTGGTTTTCAAATGTCACAAATTGAAATTGGCGCGACTTTATGATTTCATTATCTAGCCGAACGTTGCTTGTTTTTGGCATTCCAGAGAATGGGCAGCGATATCTGCGGCTAAATATAACAAAGAGTGCTGATGAAGGAAAGCGGGCAAGAAGAGAGTGCTCCGCTTGAGTCAGCGCTTGGATGTTTGAATGTTTGAATGGGTTTAGCCATAGGTTCGGTTCGCGTTATGGTTCCTAGCTGTTTGTTTTTGCTGGCCAAGAACGAGACTCCAGCGCGACTTTCTAGGGAGTT

GGGGGAAAAGTCGGTGTACACGCAAGGCCCCAAACCGCCGAGCAGAGTGTTAGCGTTTCTCCCTTTGCGCCTGGCATTCGCATGCGTAAGCCGTTTTTTTTTTTTTTTATTATTTGTTTTTTCTTTTCATATTTAGCGATTAGGTCATGCAACAGCGGCCAGCTCAAAGCGAGAAAAACGATAACAAATGCCTAATCTTGCAGGGGTAAAGCGTATTTTCCGGCTTCGATTTTTCAGCTGCAGTTTTATGAATGGATGTTGGCTGCTGGCATTATATTCCGATCAGCACAAGCACACCCCACACAACTACCGGTTGTAGTTGTTTGCATTGGCCATCGAAACCAGTCGATTGGTAAGTAGGTGTCGGTCGCATACTAAGCGCTAAGTTGCCGAGCGGCATTACCTCAGCGCACCATTTTGGCCAAGAGAGTAGGTGTTTAGGCCCGCTTCGCCTCCGTTGGAATGTTAATGAGTGCTTTTTGTTTTTAGCTCAAATATGAGTTTATTTTATGTGTAACTTTGTACACGGGAAGCCCTCTTGCTGCGCTTACCTGTCGTATTTCCTGTGTTTCGACATCCATGCTGACGGATTTTCCTGCGACGGACTCCGGCTCTCCGACGCCCGCCACTTCACTTTCCGCACTTTTTGCACTTTTCGCACTCTATGTTTGACCACAACACACCGCTTTTCCTTTTTCACTTGTCTCTTTTCTGCGTGCGAGCGAGTGCGTGTGTGTGTGAGTGTGGCTTGGAGTTTTCCTCAGCTTAGGTGATTTTCACTTGGAGCCTAAACACAAGAAAATACAAAAACACGATTGGCACTTTGTCAAACCACTTGGAAATCGAGAAAAATAACTAATTGCGCACACGCATTGTCAGCGAATTTTTTTCGGCGGGCGGGGCAGGGAAAAAGCTTAAAAGGCAGGCGGGCAGCCGAAAGAAAATGTGAATCATTCACGGAGCGCGACGGTGGGTGGTGGGCGGGGGCGGGGCTGCAGTGCGACAATAACAGAGAACACTTTACAGTTGCCTGTTCGCTTTTCTCGAGATGCTGCTGCATCAGCTGTTGCGATTTGCTCGTCTGGACAAGCCATTTACGCACTTTTCAACCCATCGCAGTGTTCA

 

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