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CRIMIC CR00699

Gene sau

CG7085

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:2228124..2228124	22D4	FBti0200637	80626

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	no band	ACGAGGAAAGAGGAAGAAGAGCAGCA	AAGCTATTTGATTGGCCTGGATGGGC

Images

Construct Design Sequences

sgRNA sequence	TCGCCATAATGGGTCACGTATGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TTCTGCTGCTGGGACTCAAG	TTCGCCATAATGGGTCACGT	CGAACAGGCAGATTCGCATT	GGGTCGTAAAGCTCCCAGAC

 

Amplicon 5'

Amplicon 3'

TTCTGCTGCTGGGACTCAAGGACAAGGAGGTGGGTGTGCTCCTTATCTCTTATCTGCTTGGGATGCACTAATTAATTGGTTTCTTTCGCAACATAAGTGCTTCTTCTTCACCTGTTAACCGACGTCTCTCTCTCTCTGTCGCTCTCACGATTTCTCTCTTTCGCAACCTCTTGCTTGGCCCCAAGTGCAACGCAGTCCATCTTCCAGCAGGGGCGTAGTGAAAATTGGATACAGGGGGGTAACTTATCACTTCAGTTATGGGGGCAACAATGTTTATCTGTCCTACAGATAAACATTTTATGCCATGTCTTAGCCACTTCTCTTAGCTTTTCCAGTTTGTTAAGCAATGCACTATGAAATCTTTTGACCAGCCTGCTGACTAATGCGCAATCAGTGAGACTGCCAACACCATTTTCTTAACCACTTTTGTTTTTGCATTAATTAATAGTGTGCTCCATCTGTTAAGGTCTAATCAGCCGTTCATTCTAATCTATAGAACCCATTACTTGCCCTTTGTAATACTTTGATGTACTTCCCAAATAAGTGATGATATATTTATATATAGTATATAATAGGTTTATTTTATGTGTTACACTATCGCGCAATTGCTTCCAGTGCCAAATCAAAAAGATTCTTAGAATTGTTGCCAACGTTCTTAGTTGTTGGAAAGATAGCGCTCTAATACTTCTTCGGTTGGCAGTTGTGGCTATAAATACATACATATCTCTTCGAGAACTTCACTCCGCGCAGCACGGAGAACGGTTTCGTCAAACGAGTTTTCCAATAAGTGGTTAATTTGCATAGGTTTGTAATATTCATAAATCTACGTATAGCGCGGGATGATGTCCGTAAATGCGAACCATCATCCATACGTGACCCATTATGGCGAA

CGAACAGGCAGATTCGCATTTCTCCATTGAATTCGGTTTTTATATTTCACTTGGCACACAGCTTTATCGCTCAATTGCTGTTCGCTTGTACCACATAGGAATGCACACATGTGCCTTTGTGGCTGGCACCACGTCTATTTGTTTTCATCATTTTGCCCGCAGTGTATTTGTGTGACCACCCACACCTCCATCCCTGCCACCTCCTCTCCCTTCGCATAAGAGGGATACGAGCTGGAAACAGTGGGACGTGTTGCCCGCTTTTGTTTTACATTCCCCTTCCGAGCGCTCTTATCGCGAAGATTTTGTCCATTTGGTTAATTGTTTGAGGAAGTACATACTATCAAGTGCTGCTCTCCTCTGCCTAGCTCTCATCCGTTTCGGGCTTTTCTTATGTCAGTACGTCACTCATTTTCGAAATTATACAAAGTACTTGGCTTCTACCCACACCATCCATTAGGAAAATTTCAATATCTCGAACTGCCGAACTGATAAGCGACGTGTTCTTTGCAAATGATTCACTTTTATACCTTTCGTTCGGAACGATTCCTTATCAATCATCTGAGCGATTGTTATATAAATTATTTTAAAATCGTGAAATGGCATTGAAACGTGGTTGAGGTATCTATCTATACCTTCCAATTATCATAGCTTTTCAAGCGTTAATAACAAGAGTAATTTTTGTAATTGATTTTTTATGTGGTTATAACATAACTCTTCTTGTTCGTTTTTTCATTTATACTAGCTAGATAAGCATTGTATTTTTTAAATTGACAGTTCATACACTTCTGTGGGCAAGGCCTAAACAAACTCAAGTGTTTAATGTTTTAACAACTGAATACTTTCGTCTGGGAGCTTTACGACCC

 

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