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CRIMIC CR00705

Gene CG5022

CG5022

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:10345035..10345054	31D9	FBti0199456	78994

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGACATGTTACAGCCGCAAATGAGTC	ATTTATGCCGACGTACTGCTGCGATC

Images

Construct Design Sequences

sgRNA sequence	TTCGGCTGTAAACAGATGGGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CTCCCTTCCCACATCGCTTT	CCGAACGCAAGGTACCAAAA	CTAACGCCCTTTCTGCTTGC	AGAGATTCTGTCCGGGCTCT

 

Amplicon 5'

Amplicon 3'

CTCCCTTCCCACATCGCTTTTCACGTTTATATTTAATTTATGCAGACTTTTCCAGTATATGGCACCCATGGGCTACGATATACCAACAGTCACATCTGAACAAACACATGTTCGCGAGGCAGTTGTCCTTATTTGGTGCTTTATACCGTTAGTAGCCTCCCCACGCGAAAAACACCCCTAACGTGAACCACTAAAGGACGGCAAAGGACACGGCCAAAGCCGAAATATTTGGATATCTAAAATAACGAATAGAATGCCAAGCATTCGGAATTTGGCTCTAAAATAAGCTGGCGCAATCAAAAATCGCCCAATTCATTTAGTGACTTTTGTTTCGAGTGAAAGTTGCCGTGAAAGTGTCGCAGTCAACACAATCCAATCCACAAAATGTTCAGGAGCAAAAATGAGGGGAACATTGTGTACAAATGCACTGTGCGACTCCTCGATGACTCCGATGTCCTAGAGTGTGAATTTCAGGTTTGTGCTGCTCTTGAACTCTGTCAATGTTAACCTAATTGGACCGGTCATTGGATGAATCAGTTGCTGAACTCGAAAATCACTGCTCATCTGCAATCGATGGAGTATTTCTTTCTATCCGAATTTGTGCTAAAATTAAAGATACTGCTATTTTAGATAACCCAGTGCACAAATATAATGTTAAATTTAATGGGATTCCGTTGTTTTTATTTTTCCGTCCATTCGCGAGTGTCTCAAGTAAATACATAAAAACATACTAACTTATTAATATATTTTGGTACCTTGCGTTCGG

CTAACGCCCTTTCTGCTTGCCGGCAAAAATATTGATGTAGTAATCCCCAAAAAGCATGCTATTAAAGCTGAAAATGAAATCTTCGAAATTTGTGGTCAATTCGCATACTTCTAAGTATTTGTACTTATAGTAATTTATTAAGCTTTGAAATTACTTTTAGCCAACTGGTACACTTTGTGGTTGTTTCTATGTAGTTCTAACCCCCATTTCACTTCCTTGAGGGTGTAAACAAGTCATACAAATTACCATTTTTTGGGAGGTGAGAGTATTTGGAAAGTGAAAATAAGTAGGAAAGACTAATGACTAGGACATATTTAACCTCTAAAGTATGCAATCATTTTCAGGAGTTTAAATATATCATATATATAAAACGTATAATAAACAAAATATAATATCAAAATATCCCATATTTTACAGCCCTTCCATAAGGGAATATACCTATTGGAGTACTTGTGCGGTGAGTTGGAGATCAAGGAGAAGGATTACTTCGGTTTGCGATATGTTGACTCCTCCAAACAGAGGGTAGGTATAACTATATATAATATAAATATTTGTCTAATTAACATAAACGTTTGTGAATAAATTATAAACAGCACTGGCTCGACCTGTCCAAATCAATTATTAAGCAATGCAAAGGTGAGCAATGTTTAAAAACGTATTAGCTTTATAAACTACAACTTTTATCTTGTCTTCAGAAATGGATCCACTATTGTTTTCGCTGAGAGTAAAGTTCTACCCTGCGGATCCATTTCGACTGACCGGAAATGCACGGATTATGCTCTATCAGCAGTTAAAGAGGGACCTTCGACATGGTCGCTTGTACTGCTCCTTGGGCGAGGCGGCTGCACTGGGCGCTCTAATAGTTCAAGGTCGGGATGAACACCTCTTAAATGAATTAACTTGCTAACTATATATATACTATTTCCTGTGCAGAGGAGCTGGGCGACTATAACGAAGACGTGCATGTGGGCAGTTACGTATCTTCCATAGAATTAGCACTGCGGCAGACGGAGTGTCTGGAGAAGAAGATAATCGAGCTGCATAAGAAGCGAGAGCCCGGACAGAATCTCT

 

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