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CRIMIC CR00706

Gene CG4972

CG4972

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:10316091..10316095	31D5	FBti0199457	78995

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATGCTCACCGGTAATGCCAAGCGAAT	TCAAAGATCGCCGGCTTCACTCTGTA

Images

Construct Design Sequences

sgRNA sequence	CACTTGAACCACTTAGATAAAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GTGCGCTGACCCACATTTTT	ACCACTTAGATAAAGGCCACAA	AGTGGATTTATGATTCTCGGCA	CGCAATAATTCGCGTCGTGT

 

Amplicon 5'

Amplicon 3'

GTGCGCTGACCCACATTTTTATGTGCACTGACGAGGGCTTTATTTAATTAGCACTTTTGTATCTCATTAAAACACAATTTTCACTAATTATTATTCTTATCCGATGTATTTTGGAATATACTTAACTAACCATGTGAAAAATGATTATCTTCAATTTTTTAGGAAGCCGCGTAGCTTCAATATCCCTAGAAGCGAGGTCTTTGTACTCGTGGAATACTTCAAGACACTGTGTGCTCACACGGTAAGTGTAAACTCTTCACTACTGTTGCTCGAAATGTTTTTAACATTGTAATTTGCATCAAACAGGTTAACCGACTTGTCAATCCATGATTTTGATAAGTTACGGCAAGGTGCAGGAGGCCTTATTCTCATGTTGCCGGCGAATATCTTGAATCTGGATCCAGAAACCAAAGAACTTATAACTATTTTGGAGCAATCAATGCTGACCCACACAGCTGCTGTTCCCATTTACTTCGCTCCGTACAACAAAGATTTGGAAAAGATCATCGATGATATTACGTACACGACTACGGACAGTCCGGGTCAAAATCAAACAGCGTTGGCACAATTGGTTGTAACCGTCTCGGCCAACAGATATCACATTAATGTCGGCGGCGGCAGCATTGCGGCCAATAAGAATAGCAAAATTCCCATTATCCACGGCGAACTGATACCCAATCAATTGGCCCTAAAACCCACGGAATCTGTTGCAGATGGACAAAAACTCCCCGTCATCTTGATAACCGCGAATCTGAAAACCTTTGGTATATATAATGTAAGTAATCTGGTGACTGAGTAGATGAATAATTTAGTCTCTGATTTTGCAACCTAAAACTAAAATAACACCATAATTCTGCTTACAAGATAAGGGCTTCTTATCTGAAAGATTGTGGCCTTTATCTAAGTGGT

AGTGGATTTATGATTCTCGGCAATGATTTGAAAGGTCAAAAGAGCTTATCTGAAGTAGATTCACTTATAATCATAATTATTTTGTCTTAAATTCAAGGATTATCCGGTGAATGCTGATGCTGCAGTTTTGCTGGTGCTGATGGAGTTGTTTAGTAAATTACACTATACATCCAGTATGGCACCGAAGTATCGACTCAGATTTTTGCTTTCGGACGCAGGCGTCCTACTAAACTTCCAAGGCTCCAAGAAGTGGCTCGAGGTTGATGATAATGCGCTGCAGGTAAAAAAATGTCTTCTAAAGGTTATATATATCAGTTTTTATCTAAAATCTTTATCTCTATTAGAACGTTGAGTTTGTGCTCTGCTTGGACACCATTAGCGAGAGCTTATCCTACACAACGGAAAATGCTTTGTACATGCATGTTTCCAAGCCACCCAAGGATAAGACTTCTATTAGCAACTTCTTCAAACTCTTGAAGTCATCAGCTGAAAAGTATTCCAATGGTCTGGCGGTCGAGGGTGTGCATAAGAAGATCAATCTAGCAGACACCAAACTGGCCTGGGAGCACGAGCGGTTCAGCATAAAGCGCTATCCCTCGTTCACTCTATCGTCTGTAAAGAGTCCAAGAAGCCCCATTCGTACAACCATTTTCAAAAACGACGAGAGTCGTCTAGTGGAGCACACTCTTAACACGACGCGAATTATTGCG

 

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