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CRIMIC CR00707

Gene Cnot4

CG31716

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:10398796..10398796	31E1	FBti0199607	79280

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGCTACAGGTGCCAAATGTCCACAAC	CCTCCTTTTGCTGGGCATTACCTGAT

Images

Construct Design Sequences

sgRNA sequence	CAACTCGGGTATTGGCCCATTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TGAGGTGGACGATCTGACCT	AAGCAACTCGGGTATTGGCC	GGCCAATACCCGAGTTGCTT	TACCTGTTGCATTCGGTCCC

 

Amplicon 5'

Amplicon 3'

TGAGGTGGACGATCTGACCTTCTTTCCGTGTACCTGCGGATATCAGGTACATACTGTCATTAAAGCACCCTTATCAGCAGCGTATTCAAGTGCTATTGCTTCCAGATTTGCCGATTCTGTTGGCATAGGATCCGCACGGATGAGAACAAGCTGTGTCCGGCATGCCGAAAGGAGTATCCCGAGAATCCAGCTGACTTCAAGCCGCTGTCGCAGGAGGAAGTATGTCGTGGTCACCTCGTATTCATTCCACTAACTTCTACTCTTCTTTAGATGATTGCCTTCAAGTCCCAGAAACGCCAAAGGGACCAACAACGAAAACAAAAGATCACCGAGAACCGCAAACACTTGGCCAACGTTCGCGTTGTCCAAAAGAACTTGGTGTTCGTTGTGGGGCTACCGCCACGACTTGCTGATGCAGACGTATGTTGATTTTGCGCCATAGATAACCGACAGCAAACCCACTCACAATTGCCATTTCAGATATTAAAGAAACACGAGTATTTCGGTAAATATGGGAAAATTCATAAAGTCGTTATAAATCCAAGTACCACGTATGCCGGGGTCCAGGTAAGAGTTGTAAGATTCAATTATTTATTACCTCTATACATTCATATGGCCCAATGGGCCAATACCCGAGTTGCTT

GGCCAATACCCGAGTTGCTTAGTCATTTTTAGGTGGTTTCTATGAAAACTTTGATAATGGCACGTTTTCAATGAAACTGAACAGATTTACGTGTATCTACCAAAATTTATTTTCGATTAAAATGAATTTGAACCTTTTGTTGTTAGCTGCTCTGGTAAGCGAACATTGTTTATAACAAGAAGTTTAAGTTTATTTTTTAACCTTTTTTATTAATATATATATGTATTTTTTTATTTTAGGCAAAATTGTGTGCGAAATGCAAAAATTTCTTGTTAATTTATGATTAAATGGATCGGAATTTACGTTCTGTTTCCGTGCTTATGCTAAAAAAATCAACTTTTGACCAATTATGATAAGAGTCTATTTTAGAATAACCCCGATTATTGTATACAAATCAAAAAGCGTAACATTTGATAAACCTGCTTTCTATAAAACTGCGAGGGTATCAGAGTTTTCGGTCCACCGAATCGCGTTTCACACTCTGCCATCATTGCTTATTTAAATTTTTTCGATTTTAAAGGGTCCATCTGCTTCTGCGTATGTCACATATGTTAATAACTCGGATGCCTTGCGGGCCATTCAAAGCGTCAATAATATTATGATAGACGGTCGGCTTATAAAGACCAGCTTGGGGACAACCAAATACTGTAGCCACTTCATGAAGAACCAGCAGTGTCCCAAGGGCGACTGCATGTACTTACATGAATTGGGCGATCCCGAGGCCAGTTTCACAAAGGAGGTAAGTTGTGAGATAACTAAATTTGTCCGTATTTAAATGTTATGTCCGGATATTTAAAGGAAATGCATCAGGGAAAGCACCTGGAGTACGAGAAGCGCCTGCACGATACTCTAATTGCGTCATTGGGACCGAATGCAACAGGTA

 

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