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CRIMIC CR00711

Gene slim

CG5186

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:18442856..18442856	55C9	FBti0199608	79281

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGTCGACCGTTTCAATGCCATTCAGG	CCAGCACAAAGATGTGCTTGCCATCA

Images

Construct Design Sequences

sgRNA sequence	GGAGGTGCCTTCCGAGTGAGAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AGCAATCTCAAATGGCCGGA	GAGGTGCCTTCCGAGTGAG	GCACCTCCTTCACCTCCAC	TATACCGGTGCGCAAATCCA

 

Amplicon 5'

Amplicon 3'

AGCAATCTCAAATGGCCGGACAGATTCCCATGTACGCCTTCCAGCCGTTGCGTCTCGTCAAGTGCCAGTACAGGGATAAACATATACCCGGTGGATTCCCTCTGGCACGCAGTGGCCACCGGATCATTGCCTCCAATTCGCATCTGTATTCCCTGGGTGGCTACAATCCGCGGAGCGCCATGTCTGCATCACGCCACGGCCGATGTCTTCTCTTCCAGGAGCTGTGGAGCTACAACTTTGCCACGAGAACCTGGAGGCTGGAGCTGAATGCGGGCAATGCCGCCAATATGCCCGTGGAGCTGGCCTCCAATGCATTGACCATTCACAATAACGTGTTGATTGTGAGTATCTATTTGATGCTAATCGCTGCAGATGGGGGACGGGGTTCGTGGGGTTTTAGGCAGACGAAAATAGATGAGTTGGATTGTCTCTTGCTCGTTGGGTGTGTTGGTAAACACAAAGAAAAACCAACTCGATTGAAAATTGTGATCTTCAAAGAGAAGCAGAAAAGGTCTGTAGTTGTAGATTTCTTTGTTTTATTTCGTTCAAATTGCTAGTACTTGAAGTGATCATATTTCTTTCTGTGTAGGTTAGGCAAACTATCAATTGCAATAAAAAAAACTTGTTTGTGCTCAAGTAGAAAACACAATTGAATGGTGAAATGAGCAGAGAAATCAGGCACAGCTGATCGCGGAGATCTCGAAAGAGAGCAGTGAATAGTAGAGTAGTGAGAGAGGGCTATTCTGGGCAATGAAAATCGCCAGGGCCAATGCCACGCCCTGTTCCGCGTCTCAACACCGCCGCCTTTGCTTTTTGTTTATGCATTCTAAACACCCGAAATGTCAACCCAAACAGCTGCCCATGGCCATTGCCGTTTGCCTCTCACTCGGAAGGCACCTC

GCACCTCCTTCACCTCCACCATCTCCTCCTCTGCCTCTGCGCATGCCAGGCAACCCTCTTTTTCCATTTTGGTTACTACGCAGTTGTTGTTTATGCTGCTGCCACATGCAACAACTTGTTTTCCAGCTTTCCCCACCTCGCGCTCGGCTGGAAAAACTGAAAACATTGTATTTACCATTTTTACCCACCCAGCAGCTGAGTGCGCGCGCTTTTTTAGTTTTCGGGAGGAAACCGAACCGAAAGCAAAGTCAAACGGGAGCTGTTGGCATTTCATGCTGGCATCAATGCCCTTCCCTTGCATTCCACAATTAGCTATAATTTGATGACGAATCATGCCCCGATACATACACAAAAACACACTGGAGACACATGTTTCCGCTTATCGGGGCTGCAGCTCAGCCTCCACCCCCCTCCCACAATACTGCTGGTCCCTCCCCATTTTCCCATTTCTCTGCTCTTTCACCACCAGGCGATGATAAGCTGCTGCAGCTCAAGTATCTGCTAATTAAATGCATTGGCAGCAATGTCAGGGACGTGGGTGCCACCCGCTTAACTCTACATACATACATATAACTATATATTGAAAAAATCCAGAGGTTTAGAAAGCTAAATTGTTTAATAAACAACGAGTTTTTTACTTCTTAGAAGAGTCATAACGATTTAAATGTAAAGTCTAGGAAAGTGGGCTTGTATACATTTTGTTTACTCTTTATTATTATTTTCTATATATCTTAGTGTCAGCTTTTCTTGAAAAGAAATTAAATTCTAATTTTAATACTACTCTTTCAGTCTCATGGCGGCACTGGTTATCCATTTGGAGTATCCTGCTCCAACGACTGTTACGTTTACCGAACGGCCAGCGCTGGCGCGACACCGGGCGTGGATCGCCTCCAGGTTAAGGGTGACCTGCCCACGGCTCAGTACGGACCGGGAATAGTGATCCACAAGCACTTCCTCTACACAATCGGCGGAACGACGGGATTTGATTACACTTGTGATGTTTATCGTTTGGATTTGCGCACCGGTATA

 

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