CR00712

CRIMIC CR00712

Gene esc

CG14941

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:11828552..11828552	33A2	FBti0199609	79282

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCGCAAGCAGCAACTCTCCTAGTCTT	GCGCCATCAGATGGAAGTTGTTTGTC

Images

Construct Design Sequences

sgRNA sequence	ATCGTTACAGGCTTTCAGCATGG
sgRNA2 sequence

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TAGTTGTCCCGGTTTCCAGC	GCTTTCAGCATGGTTTCACA	GCCTGTAACGATTGTTCTTACTT	TGACTGGGGTCACACCATTG

Amplicon 5'	Amplicon 3'
TAGTTGTCCCGGTTTCCAGCAGACAATGGCATTTTCGCAGGACTTGGAGAGCACAAAGTTGCCGAACCACTGCACACAGTCCACATAGTTCCTGTGTATATCCCGCGTTGAAAAATCGGGGAAGTGCTTGGTCACCGTGGGGAAAGGCAGCGTTGACTTCTCCTGGCTGAAAGTGTTCGACAGTTCGATCTTGTGATGGAACTCGGGCGTATTCAGACACCACAGCTTCAGCGAGTGATCCATGCCGCTCGACACAATGCGATCTCCACGCATATTAAAGTCGATGGACAGCACCTCATCGCGATGTCCCTCCACTCCGCCCAGGATGGCAATGCACACATGGCTCTGGATGTTCCACAATCTTATCGCGTGATCCTTGCTGCCCGAAAGCAAAAGCTGTAGCTTGTGCGGATGGAACTTCAGCTCGTTAATGGCCTGTCCATGGCCAATGTAGTTGCCCACCGCCTCATTCTGCTCGACATCAATAACCCGGATGACTCCGCGGTATCCAGCAGCGGCCAGGAGAGGTGAAGAGGTCTTAAGGTCGTACGACCAGGCGCAGGTGTAAAATACTTCATCGGGCTGGGAAGGGAATTCAAGATTTGTTGATGTATTTCGAGTTTGTACAATTACATTTGCGACCATATTCTCAGTTACTCTCGGCTAAATTAACAATATAAGCTTTAATTTGAAAACTTGACTGTTATGAAAAACCAAACTTAATACAAGAAATGATAAATGTTTGGATATTGTGAAACCATGCTGAAAGC	GCCTGTAACGATTGTTCTTACTTTAAAACAATTAACAAAAGCAATGATTTAATAGAAACTTTGACTGGATCCCACTTTTTCTACAGCTTTGAATGCGATGTCTAATGTATGAAGCCCCTAACTTTAGCCAACTGATCAATAGGGTCTTGAAAGTGTATTCTGTATCCTGGCGACTCACATCCGGATCTGCATAGCAGTGGAGCAGCTGCATCCCGCCCTGTCGAGGGCACTCGTAGACGGTCACCCGGTTGCTGCCCGCCGTAGCGAAGACCTGTGGCTCATCCTTGCCCAGCAGCGTGTTGAAGGCTACGCCGAAGATGTTGGCTCCGTGGTTCTCCTTCACGTGGGTGCTTCATGGTAATCATTTGTAGGTTCAGTATCAGTGGCCAGCTGAGAAAGTGCATATTCTTACTCGTATTTGTAGGCGGCGCGACTTTTTGGCTTCGATTTGGTGCTCCGGCGACCGCGGCGCTTGCTGCGCGTGCTGGATGACGGGGACTTGCTGCGCGAGGTGGTGCTGTTGGTGGTATAGCTGGCCGACTCATCCTGCAGCCGGCACATGGCCCAAAAGATCCATTAATTCTCGGTTCGCACAGCCATTTGTCATTCGCTACTCACGCCGCATGATTCCTCCGACTCCTCGGGCTCGTTGCCGTTTTTCACTTTATCACTGCTCATTTCGAACGCGCGGCACGTTTGTACGGTTAAATGAAACCCAAATTAGGCCGCGACTTCAATGAACTATTCGATAATGCTTTTTTTAAATGTTTAAATAACGAGGCGAATGAAAATGTTTTTTTTTGGCGGCAATGGTGTGACCCCAGTCA

Amplicon 5'

Amplicon 3'

TAGTTGTCCCGGTTTCCAGCAGACAATGGCATTTTCGCAGGACTTGGAGAGCACAAAGTTGCCGAACCACTGCACACAGTCCACATAGTTCCTGTGTATATCCCGCGTTGAAAAATCGGGGAAGTGCTTGGTCACCGTGGGGAAAGGCAGCGTTGACTTCTCCTGGCTGAAAGTGTTCGACAGTTCGATCTTGTGATGGAACTCGGGCGTATTCAGACACCACAGCTTCAGCGAGTGATCCATGCCGCTCGACACAATGCGATCTCCACGCATATTAAAGTCGATGGACAGCACCTCATCGCGATGTCCCTCCACTCCGCCCAGGATGGCAATGCACACATGGCTCTGGATGTTCCACAATCTTATCGCGTGATCCTTGCTGCCCGAAAGCAAAAGCTGTAGCTTGTGCGGATGGAACTTCAGCTCGTTAATGGCCTGTCCATGGCCAATGTAGTTGCCCACCGCCTCATTCTGCTCGACATCAATAACCCGGATGACTCCGCGGTATCCAGCAGCGGCCAGGAGAGGTGAAGAGGTCTTAAGGTCGTACGACCAGGCGCAGGTGTAAAATACTTCATCGGGCTGGGAAGGGAATTCAAGATTTGTTGATGTATTTCGAGTTTGTACAATTACATTTGCGACCATATTCTCAGTTACTCTCGGCTAAATTAACAATATAAGCTTTAATTTGAAAACTTGACTGTTATGAAAAACCAAACTTAATACAAGAAATGATAAATGTTTGGATATTGTGAAACCATGCTGAAAGC

GCCTGTAACGATTGTTCTTACTTTAAAACAATTAACAAAAGCAATGATTTAATAGAAACTTTGACTGGATCCCACTTTTTCTACAGCTTTGAATGCGATGTCTAATGTATGAAGCCCCTAACTTTAGCCAACTGATCAATAGGGTCTTGAAAGTGTATTCTGTATCCTGGCGACTCACATCCGGATCTGCATAGCAGTGGAGCAGCTGCATCCCGCCCTGTCGAGGGCACTCGTAGACGGTCACCCGGTTGCTGCCCGCCGTAGCGAAGACCTGTGGCTCATCCTTGCCCAGCAGCGTGTTGAAGGCTACGCCGAAGATGTTGGCTCCGTGGTTCTCCTTCACGTGGGTGCTTCATGGTAATCATTTGTAGGTTCAGTATCAGTGGCCAGCTGAGAAAGTGCATATTCTTACTCGTATTTGTAGGCGGCGCGACTTTTTGGCTTCGATTTGGTGCTCCGGCGACCGCGGCGCTTGCTGCGCGTGCTGGATGACGGGGACTTGCTGCGCGAGGTGGTGCTGTTGGTGGTATAGCTGGCCGACTCATCCTGCAGCCGGCACATGGCCCAAAAGATCCATTAATTCTCGGTTCGCACAGCCATTTGTCATTCGCTACTCACGCCGCATGATTCCTCCGACTCCTCGGGCTCGTTGCCGTTTTTCACTTTATCACTGCTCATTTCGAACGCGCGGCACGTTTGTACGGTTAAATGAAACCCAAATTAGGCCGCGACTTCAATGAACTATTCGATAATGCTTTTTTTAAATGTTTAAATAACGAGGCGAATGAAAATGTTTTTTTTTGGCGGCAATGGTGTGACCCCAGTCA

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