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CRIMIC CR00713

Gene Fak

CG10023

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:19432574..19432578	56D5	FBti0199610	79283

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TAATTCATCGGAGACACTGAAGCGGG	TTTCTTTTGTGGCGGATGGCGGGAAT

Images

Construct Design Sequences

sgRNA sequence	TTTGGGCCTATTGGACACGTTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ACCTTGTGACAGCGCCATTA	TGGCAGCCAAGCTTAGCATA	TGTCCAATAGGCCCAAACCTG	TCCGCCATCGAAACCTTCAA

 

Amplicon 5'

Amplicon 3'

ACCTTGTGACAGCGCCATTATTGATTTGACCACCAGTGTGGTGGCGCAATAAACTTCGTCATCTGCGCGGTTAGGTGGTCGACTTGGAACTGCACTGCCCAGGTTTCGTGCATTTGAACCCATCTGTTCTTCTGCAGATGTCAAGCTGGACAAATGATCCGCCGATGAGTGGTTCGACTTCAGTGAAGAAAGATTGGCATTTGGTGTCATAGGTCGAGAACTGGAGTTCGAGCAGTCCGGCAAACTGGAAGGACCAGCTGACATGAAGTCAAGCTTAAACATTTGAGCCTGATGATCATTGAGCGATCCTTGAGGTATTGAGCAGGATCTCTGCCGCTGAAAATTGTATGATATGAAACCTACATTCTTAATAGTTTTTATTTGCTACTCACTAGCAATTCTTCTTGAATTAACCATTCGCTGTCGCTGTTGCTATCCTTTTGCTGTTGTCGTAGTTTTTCGCCCAAAACATTGTGAATGCCCTGGAAATAAAAAGGATTTGTTACTCGCGGCTCAAGGATTTCAAGATTTTAAGTTAACTAAAACTTAAATAAATCCAAGCTAACTTAAAAATGTTACTAAATATAAATAAATATATAATGCTCTTTAGCATAAAAGTAAAAAACGAAGGGAAATAAAAAGGATATGCTAAGCTTGGCTGCCA

TGTCCAATAGGCCCAAACCTGATTTCCCATTGCTTCAGCTTGCAATGTGGCCAAGCAAGCAGCTCCTTCGGAAATGGAAATGTTTGGGTTAACTGTGATGCTCTGTGGTCCCGAAGTCATCCACTGTCGCTCCATTTCTCTCTGATGTCTTAGACTTTCGTCCAAGTTGTGTGGGGATATAACTCCAAAGGAGTCCATTGGAATATTCTGCTGTAATCCCATGCAATCTGTGTTTTGTACTTGGCCCAGTTCAGCTGGGCGCACATTAACCATTGGGCTAAACTTGGGTTGCTGCCTAGCGGACACACAGGTCTTGAGTCCGATGCCCCCAAAATCGTAAATCTCCTCGGGTAGAGCTTCGCATTCAATAGAGGTTTTCATCTGTTGGCGATAGGCATGGAAGTATGTGTTCCGCTCCATGCTCCTCGATCGCTCTGCGGTATTCGTGCAGTTTGGTGGCGGCTTTGTATTGTAGAAAACAAGATTTTGTGCTGACATCAATGACTGGTGGCGTTCCAGGCTGCCACCTTTGGAGTGCATGGCTTTTGCTGGTGGTGGTTGGTGCCTCTCCAAGCTCCCATATAGGCTATATCCGGACACCTTGGACACAGACGGGTTAGAAATGTTTTCGGCTTGCATGTGTTCCTCTGTTTGGGGTGGGCAAACGACGAAGCTGCTCTTTGAAGGCAAACTACCCGTAAACATTCCTGCCTGGTGTGAATTGAGGGATTCATGGGGGTTAACTGAAGCAGTAAGGGGGTTAGCCAGTTGACTAGCGCTGCTATTAGCTGAAAATTGGGACCTAAGCCTTTCGGATTCTGCGATGGGGTCAAGTTTTAACAAATCTGCTGCTGGTAGCTTAGTAGTCTTAAGCGAAACATTCGGATTGCTGTCATCTAATCCTACGGCTAGAGTATTAAATACCGAAGCTGGTGTGGTGTACGCCGCTGGATTTATGCCTCGTTTTTGGTTCTCCATCATCAGTTTGGCCAGCACCGCGGGATTTTGTGCAATAATATAGGTCTGAGGTAGCCCCGTTGTTTCAGGCATTAAGCCAGTGATGTCTGCAAATAATTAAAACATTAGAATACTTATATAAATATTTACTTAAATAGAATTAATTACCAGGATCGTGCATCACCCTTGAAGGTTTCGATGGCGGA

 

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