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CRIMIC CR00714

Gene 128up

CG8340

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:12037537..12037543	48D8	FBti0200642	83185

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TAAACTGGCCCACGAGATGGACAAAG	TCTTCACAATCTGGACCACATCCTCG

Images

Construct Design Sequences

sgRNA sequence	GAATCCAGGGGGGAATCCGCGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TTTGTTTCGCACGAGCAAGG	GAATCCGCGGGGTAAATGGA	TGGATTCTATCTAAATCACCGGG	ACAGATGAGCCCCAAACCAG

 

Amplicon 5'

Amplicon 3'

TTTGTTTCGCACGAGCAAGGTGTGTTCTCCACGTTTTCCCACAATGATCCGTAGCAATTTTCCTGAAAAACGATAATTAATGATTTAACTTGCCAAATTTAGCGATATTAATCATTCTGTTTAAAATGTATATAGTAATTTGATTTTGAATGTATTTCAGTTCAGACCCACCGTAAACATTCCAAATAGCATTAATTTCCGGATACATTTTTTGAGTTTTAAGGCAGAAATCCACGCGACCAATTAAACTAATTAAGTTTCGTCCTGGCTTCAACATCGGGGAGCTTGGCAACTCCGCTGATTCCTTTTTGGCTGACATTTCGCAACTGGATTTTTCCAGAATAATCGGTGATGGAACGTTCTCCTTTTTGGGCTGAACTTTCACAGCTTCCTCCACGAATCCATCGTATATTAGCTTATTATCCTTACGACAGACAGTTAGTTTTGTTTGCCGCAATTGGGTGCTATTTTTGCGCAAAAGTTCAGCGGCCGTTGGAAAACTGCGATTCAATTGCGAGACGTTGTTGGTCAGTTGGCTCTTAAATTGAGCTTGGTTGTTACCCAGAGGGGTGCGCGGTTTAACTGAAACAATGTTAATTTTTTGTTAATTTATAAATAGTTCTCAGTATTTAATTGCGGAACTTAACCTTTAGCAAACATTCTTGCAGCCATCAAATTGTAACGGCTATATTCCAATCAGCCTGACCATTTTAATTTTGTCTAAGAATACTTTTACGATTCGTTAAGTGTTGGCTAACGTTGGGAATCGACCACATTACTGAACAAATCGATTGTATCGAATACATATCGATTGCACCTCCAACTGTCCACGCGAGAGTTTTATATATTTTATTTTTACATGCATATTTGTTGATAACTGGGGTTTTCTGTGAACCGCGTTTAACTCTCAGCCAGCCATGAGCACAATATTGGAGAAAATCTCGGCCATCGAGTCGGAGGTGAGTGGAACTTGGAGTACCTGCCGATCTTACAGAAACTAACCTGTCTCGCATCCATTTACCCCGCGGATTC

TGGATTCTATCTAAATCACCGGGTTGTTGGACCACCTTCTCAACTGAATCCTAGATGGCCCGAACCCAAAAGAACAAGGCCACCTCGGCCCATTTGGGTCTACTGAAGGCGAAGCTGGCTAAGCTGCGACGCGAACTGATTTCCCCCAAAGGAGGCGGCGGCGGAACCGGCGAAGGTAGGCTCTTGGATATATAATTCAGGCAATCACTAAACATTATGTATTTCCAGCTGGCTTCGAGGTGGCCAAGACTGGAGATGCCCGGGTGGGATTCGTAGGGTTTCCTTCTGTGGGTAAATCCACACTGCTCTCCAACTTGGCTGGCGTTTACTCCGAGGTGGCGGCATACGAATTCACAACGTTGACCACTGTGCCGGGATGCATTAAGTACAAGGGCGCTAAGATCCAGCTGCTGGACTTGCCCGGTATCATTGAGGGCGCTAAGGATGGCAAGGGTCGAGGTCGTCAGGTGATTGCTGTCGCTCGCACCTGTAACCTCATTTTCATGGTGCTGGATTGCCTGAAACCGCTTGGCCACAAGAAACTCCTCGAGCATGAATTGGAGGGCTTCGGCATCCGGCTTAACAAGAAACCACCAAATATCTACTACAAGCGGAAGGACAAGGGTGGCATCAATCTGAACAGCATGGTTCCGCAGTCCGAGTTGGACACGGATCTGGTGAAGACCATTCTATCCGAGTACAAGATCCACAATGCGGACATCACCCTGAGATACGACGCCACTAGTGACGACCTCATTGACGTTATCGAGGGCAACCGCATCTACATACCCTGCATATATCTGCTGAACAAGATCGATCAGATCTCCATCGAGGAGCTGGACGTCATCTACAAGATCCCGCATTGCGTGCCCATCTCGGCCCATCACCACTGGAACTTTGACGATCTGCTGGAGCTGATGTGGGAATACCTGCGACTGCAGCGCATCTACACCAAGCCCAAGGGCCAGCTGCCCGATTACAACTCGCCCGTGGTACTCCACAACGAGCGCACCAGCATTGAGGATTTCTGCAACAAGCTGCATCGCTCCATTGCCAAGGAATTTAAATAGTGAGTGCGCTTCACTTTCCCTCTGTTCCCTTGGTTATAATAACATTTATGTATTTCCTTCGTAGTGCGCTGGTTTGGGGCTCATCTGT

 

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