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CRIMIC CR00717

Gene CG10602

CG10602

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:18857157..18857157	37B1	FBti0199611	79284

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAAGTAATCCCCAACGGCCATCCAAT	AAAACGGTTGGTCCGCCAAACAAGTC

Images

Construct Design Sequences

sgRNA sequence	GAGTACTTGGGGGAACGCACTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GAAGCCCTCGTTCAACCAGA	GTGAGTACTTGGGGGAACGC	CGTTCCCCCAAGTACTCACA	CGAGAAGGATCCCACGATGG

 

Amplicon 5'

Amplicon 3'

GAAGCCCTCGTTCAACCAGAAATGCTCGAAGTTCTTGTTGGTCACAAGATTACCAGTCCAGCTGTGGGCTATCTCGTGAGCCACAACGTCGGCCAGAGACTTATCACCGGCCAAAAGAGTGGGAGTTACGAAGGTGAGGCATGGATTCTCCATGCCACCAAAGGGGAATGAGGGGGGCATCACCAGAAGATCGTACTGCTTCCAGACATACGGACCACACAACTCAGTGGCAGTCTTTAGCATGGTAGCTGTTTCGGAAAACTCCTCGGCGCAGGCATCCACGATGGCTTCCTCAGCCCAGACACTGGAGTTCTCCCCCAGCGGGCGGGAAACCAATTTGCCAATGGCAATGGCCACCAGATAAGCGGGAATGGGCACCTCCTGCTTGAATAGTGTCTTTCCCGGTTCCTTCTTATCGATAAGGGCGCTCATCAGGGCCGTGAGCTCGCTGGGATGCTCCACTGTGGCATCATAGGTAAACTTAACAGCTGGCGTGTCTTGGCAGGGAATCACCGAGCGAGCGTGGATTGCCTGGCATTGGGAGAACATATAAGGATGCTCCTTGCCCAAAGTCTGGGTGGGATTCAGCCACTGCAACCCACTGGCGCTGCTTGAAGTCTCGTAATCGATGCGAACGTTAAGGCTAGATAGGAGTGTTTTAGATTATTAATGACTTTCATAGATTAGATTATCTATCGCCGAGGTTTTACCTTCCCTTAGCCGTTCCAGATGGCAACTCCAGCGTTAGCTTCTGGCCAATATCATCGACGGCATCGCTGATAAAGAAGTTGATCGGGAGCTCACTGCCTCCGGCCAGCAGCGTGGCATTGGTTACATTGATGTCGCGGACATCAAGGAGCTAAGTTACATGTAAGGTAAATGGGTAAATAAGAAGTTCTAGGAACATGCCGCGGAATGAATATCTTCCAGAATTCTTATGCTGGATCCAGTGCGTTCCCCCAAGTACTCAC

CGTTCCCCCAAGTACTCACAATTTTGTCCAGATTGGCAGTCAACACCTTGAAGCGATGGAGCACGCTTCCTTGGATTTTGGTTGCTGCAAAGTCGATCTTCCAGTTGAGAGCACTGTGCTCAGTGGTGATCAAATCGGGCTGCGAGTAAGAACTGGGATCCACGACTCCTAAGCGACCCATGTTGCGTTTTTGTACTTGGTAAATCGGGAGTAGTTTGTGTGAAGTGCACAAGCCGCGAGTAGAGACTGAAAAAGAGTAAGAAATATTTGAAAATTGTTAACTTTCTGCGATAGTGCTATCGTATGCCATAAAAATGTGCACAGATAAGCCAAAAGCTGTGGTTAAATTCTAACAGCTGCTGTTGTGTGGTTTAAGATATTTACGTTAAGTGATGACCGCACTTTCTATATAGTATATTTATTCACGTACGTTAAAATAATTCGTGGGCGGCGTCGATAGATATGGGTGCAAAATGAAGCAGATACAAATGAGATGAATAAGTTGCAATTCACTGCCACACAGCCGGTAAATTGCAGGGTTGTCGAGGATCGCAAAGTGTTAGACACAACAATCTATTAATTATGAAATAAATTAAGCAGCTTGCCAAACTAAATACGTTATTTCCAGTGCCAAGTTACTTTCTTTAAATTCTTGCTATTCTGACATAAATTGTAAAGTCTATAAAAATTAACCTGAAGCTTAGCTGCCTGGCATCCCTTTTGCAGTATTGCCAGCCCAAAAAAATCTCACGCTTTTAGATTAGTGAAGTCGCCGCCGTTATACCATGTAATAAACAATAAAATCACATAAAATTTGGCTATAGAAAATAAGAATTGAAAAAAAAAAATTAAAACTTCAATAAGCTTTTTCCATATTTTGTATTTATCTTAATTACAATGATTTCCTATCAGCATATCAACGCAAGATATAATCCTTGGGATAATCCATAATGTTGGGGAAGTTCTCCAGCGTTTCTTTGTCGATATGATCCAGACGCTGGGGAATAGAGCGCGGCGTGCGGATTTGATTCGTGACGTTTTGCAGGATTTCCTCGGGCACCTGATCGTCGGCGAACAAGTGCAGTCTTTGCATGGTGTGCCTGCGCTGCAGATTTCCGCGCATCGAGTTGTACACGGCCTTCTTCATCACCATCGTGGGATCCTTCTCG

 

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