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CRIMIC CR00718

Gene Fem-1

CG9025

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:20665191..20665217	57A9	FBti0199612	79285

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGGAATTGGCCAAATGCCAGACCAAA	AAAGCACCTGGCACTGGTAAAACCTG

Images

Construct Design Sequences

sgRNA sequence	AACGTGAGGTATGAAATCTCTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AATGCGTTCCCTTATGGCCA	CGTTTATCACTTGCGACGCA	CATGCGCGCTTTCTTTGTTC	ACTCTGCTTCCACCCCCTAT

 

Amplicon 5'

Amplicon 3'

AATGCGTTCCCTTATGGCCAGCGACTCCATGTGGATGGAGTGGTGATTAAGTTTGATGGCATACAATTCGGGCAGATTTTCAGTCTCAAGCCAATTGTCATACGCGGGCACAGTGGGCTGAACTCTTTTGCGGATCACACCGCGTGGATTGCTGTAACGTAGCTGCATGGCTCTCAGCAGATAGCCATAGGCCTTATTCACGTCGTATTTGATTTTGTCATTCGCATAAGTGGCTCCCAGAAGCTCCAATGCTGTGATCTGCTCCTCCAAGCTCATCAGTTCTGGTCGTTCGATGAACGCTTCAAGGACATCCTCATGGAGACGTTCGGCAGCCTGCAAGGCGGGCGTAATTCCCAGCTCATTCTTCTTCAGAGTGGCGCCATGGTCAAGCAGGGCTACCACCACATCCAGGTGACCGCTTTCGGCCGCATAGTGCAAGGCAGTGGCGCCGCACAGCGCCTGATCATTGGCCCTTGATCCATTCTGAAGTAGAGTCTTTACCACCAGGTGGTGACCCTTGTAGGCGGCAATCATAAGCGTGTTGTTGTTGAACAAATTCGTGGCATTCACATCAGCGCCATTGTCAATTAGGAATTCAACGATGTCCAATCGACCTGTGGAAATATTCAAATTAATAACATATCCATGATTATGAGTGAAATATACGAAGTAAGCGTATAATTCATAGCACTTCCGTATAGTGAGAGCTTTTGGATGCTTACCCTCATAGCAGGCCGCTCTCAGCGGCGACGACTGCGCCTTGGTATTGTGATTGATGGCCGCTCCCGCTTGGACGAGCATTTTGACTATCTGCAGATGCCCCATTCCGGCAGCCACCCACAGGGCTGTGGCTCCGTAGACCACCATCCCGTCGTAGATGACATTGCATTCTCGGTCCAAGTCGACGTCGTAGTGCGACAGCAGAGTCTTCACGAATTTCACATTGCCCATCATCGCGGCCATGGTCAGGGGTGTTAGCGATTGTCCATCCGGCTGATCGAAATTCTGGCGATGAAAAATGTATAGAAACTAGAATGTTTGGAGGGGCCAGGGAAAACTTATGATGTGCGTGCGCAGCCGTTTCACAGTCAAGGTTGGACGAAAATAGTGCGTCGCAAGTGATAAACG

CATGCGCGCTTTCTTTGTTCTTTGTGCTTTACATGGGCGAAATGGGACTTGGGCAAAGTCCATAAACTTGGCAACTGCGTTGCGGCACGGTTAGAGCAGCATCTGAATGTGGGTCAAGGAACTGCTGGCCCGGGACGGGACACTCACCGCCGGATACGTGATGTAGTTCGTCACGGGCAGCGTGTCGTTGGCTCCTCGCCAGAATCCTGCTCCAGGCCAAGCGGACTCATACCACCGCTTGCGAGTGCTGCAATTAATTTGTGAAAAGCCATTTGATTCGAGATCAATTCTAGCTGGAGCTTGGGCATAAAACCCAACTATGTATTACCGATACAGCTCCGCGTGCATGGCCTGCAATCTGAATTTTAACTGACAGCTGCCTTGACAGTGGAGTTCCGAGCACCGAAAAACTCGATAAAACTTCGCCAGGCTCACGATTCCATTCTCAACCCTAAGTTTGCATACGCTTCCAGTATTTTTGGGGGTTGAATCGCACGGAGAGGCAGCGATAGATCCTGCGCAGGGCCAAACAGCTGTTCAAGGAGAGAGGTCCTTGAGGGCTTGTTTACCGCCCGCAGCTGTTTGCCTGCCTGCATTCCAGCAATCCATTGCAGCTCGGAGAGAGTGGATACGATAGGATAGGACAATAAAATAGGGGGTGGAAGCAGAGT

 

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