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CRIMIC CR00719

Gene RpL18A

CG6510

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:17547070..17547094	54C3	FBti0200643	80630

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	no band	GATTTCACGGAAGTTCGCTGCACAAC	GCGGCAAGTCCATCTATGGCAAAAAG

Images

Construct Design Sequences

sgRNA sequence	ACAAACACACGCATGGCTGTTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ATCGTGGAACTGCTTCACGT	TGTGTTTGTTTGCCTGCGAC	GTTATGTTTTCGGCGGCACA	TAGTTCGATAGCTGGCGCAG

 

Amplicon 5'

Amplicon 3'

ATCGTGGAACTGCTTCACGTGCACGCGGCGGGTCTTGGCGGCAGGGATCGAGTCCACCTTAATGATCTGGATGGAGTGGGCACGGGCACGGTGGCGGGCTCCCATGTCGCGGTAGCACTGAGTGACGGCACCGCCGACAGTCAGGTCACGGTACTCGCGGTACATGTTGTGGGTGCCCGAGCGGGAATCGTAACGCAGCCAGATGCCGAAGTTCTTGATCTTCACGGGCGACGTCTCGTACACCTGCTTGATGGACACGATCTCGCCGGTGGTCTTCTTGAACTTCTTCAGTTGGCGCAGGAAGTACCAGAAGCGGGATTTGGCCACGATGTTGTCGGGGGCAAAGATGCGCATCTTGTAGAGGGGAGTCTGGGGCTCCTTCTCGCTGGGCAGCTTGCGGCCCACGACCTCGTACTCCTTCAACTGCAAATGGGATTTGGATTAGTTTAAATGGTCTACATAAAACAATTGTAAACAAATGCGAACAGTCGCAGGCAAACAAACACA

GTTATGTTTTCGGCGGCACAAAGCAGTTGTATTTCTATTATATTGAATATGTTTGAATAGTTAGTTATAAATTCGTACTGAATAATAGTTTCTCACAATTATGTAAATTACTTTTGTGAGTTAGATAGGATCACATTTTTAAGAAAAGTGGCTCGGCAAACATTGTCCACGACAATGTCGTTTTTTACACAGAAATTACGGAGTTTTAAAAAATCCAACGATTACGCCAAATAGGCTATGTTTTTGCTTATCTGTTGCACGCATTTTAAACTAAAATAATGATTGTATAAATCATTTATTTTGGCAAATACTAACCAATCCCTTGGCTCTCATGTTGGACGTTCACGCGAAAAGGAAACGGAAAGAGCTTGTCAAATCGGTATGACACTGAACCTCGATTCAGGGATGGAAAAAACTATCGATAGCTAACTACGAATATTAGTTATGATTGATCCACTGTGATACTGGACGTGCTATAATGGTTCGTTTAAAATTAAGATAATTACTCAAGATAATATTTTTAAATAGCCCGTGTAAATAAGATTTTAACAATAATTGCTGGTACTGGCCAAGTATCGATATGCTTACATTGAACCGCAAGGGAAAAGCACAGCGATGCCTGTCACACGGTTGCATTTTTGGCAGTCACACCAACACGCTGCGCCAGCTATCGAACTA

 

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