CR00722

CRIMIC CR00722

Gene ref(2)P

CG10360

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:19543194..19543209	37F1	FBti0200644	83186

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	TCACGTGAACCTCGCGATTTGGAAGT	TTCAGCTGCCTTTTCAGCGGTAACAG

Images

Construct Design Sequences

sgRNA sequence	AGGTGAATCCCTGCTTTGTTGGG
sgRNA2 sequence

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ACCCCAGTTCAGATTGACCG	ACGCAATAATTTGGGCTGGT	AGCAGGGATTCACCTAGATAGT	CTCGGATCGTCGACAGAAGG

Amplicon 5'	Amplicon 3'
ACCCCAGTTCAGATTGACCGTTTTATTGCCTCCCATTTTTATAGTTCCGAAGAAAATTACGAACGCAGAGGCATCATTTTAGATTACCTAGATTGCCAAGTTTCATAAAACAAAACCATCTTTAGATTAGGTTTTCAATATTTCGGGTTTGCTGATTTCGATATCGATAAGAAACTGACGTATGTATGTGTGAAGTTTTAATGGGTAAAAATATCGATATTCTGGCGAAATTTTATGTAAAGCAAAATGTGAATGTAAAAATACTAAAAGTGGTGAGTTCGATAGAGCCGCGTAAAAATATTTTCATTATCGATAACCGAAGTATTATTAATCTTTGCCAAAGACCAGCTTTGGCATTACAATACAATTTTGGGGATAGATTAACTAGTGCAGCCCAGCAGGCAGCACCGTGATTTGCAACAATTTGAGCCTCACTCATTCAAGAACCTTCCAGCAACTAGTTCGCCTAGTATATATACCTGCTCTCACCCAGCTGCACTTGTATACACAATCGAACGGCCGAACAAAAACTACGCAGTAGTCGAGAGCTCCAAATTAATAGAATCCGTCACATAAATTCGCCGCAACAAGTTAAACGAGAATAAAAGCGTCAGCATCGATATATATACATACATATATTCAAAAGTCTGTCGAAACTACAGAATACGTTGCGAAAATGCCGGAGAAGCTGTTGAAAATCACCTACCAGGGCGCTGGACCCCAGAAAAAGATAAATGCATACCTGCGGATGCCCTCCCAGAATTACACCATATTGCGTCGCGAAATCGAGCTGTATCTTTTCCAGGAGCGCCAGTTACCCAAATGCGACGTAAGGACCTTCTGGATCGGTGAGTTTTACAATGAAAATGCCGCAGAGTGAATGTTTGCTATACATACAAACAGTTATTCGCAATTGCAATCGAGTCGCAAAGATTGCAACATATGTGCATGGTATAAGTACATTCGTTTCCATACATAACTGCATCCGTCCGAGCAGTGCTGACCATTTAGCTTTTAAGTCTCGTTACTTAAAAACCAGCCCAAATTATTGCGT	AGCAGGGATTCACCTAGATAGTATTCCTGCTCAATGCTTATCATTGGCAAAATTGTGTTGACACATTAGCTGATAAATATGTACATATATATTTTTATGGTACAAACGTGGTCAACATATTAGATGCACTGTTTCATAAATGGCACACATACAAAACATTTTTGATTTTACATATTTCTGAATTCTCGAATTATACATATATATTTTAAATACATCTAACGCGCTTTTTAATTTGTAAAGTATGTGTACATGTACTCATATACATTTACATAGATCAAACGGGTTGTGCTGGTACAACCACAACTGTAGCTAATTTGTCTAACTAGAGCCCTCAGTGATTCACCTTTGATAACAAAACTTCACTGATTCATAATTGATTTTATTTTCCTTTGCCTATTCTAACTTTTGCAGACGCTGATAAAGATGAAATCGAAATAGTCAACCAAAATGACTATGAGATTTTCCTGGCCAAGTGCGAGAGCAATATGCACGTTCAGGTGGCTCCACTGGCGCCCGTAGAGGAGCCAAAGGCCACCAAGCAAGAGGGTTCTTCGGCCAACGCTGAAGCTCCTTCTGTCGACGATCCGAG

Amplicon 5'

Amplicon 3'

ACCCCAGTTCAGATTGACCGTTTTATTGCCTCCCATTTTTATAGTTCCGAAGAAAATTACGAACGCAGAGGCATCATTTTAGATTACCTAGATTGCCAAGTTTCATAAAACAAAACCATCTTTAGATTAGGTTTTCAATATTTCGGGTTTGCTGATTTCGATATCGATAAGAAACTGACGTATGTATGTGTGAAGTTTTAATGGGTAAAAATATCGATATTCTGGCGAAATTTTATGTAAAGCAAAATGTGAATGTAAAAATACTAAAAGTGGTGAGTTCGATAGAGCCGCGTAAAAATATTTTCATTATCGATAACCGAAGTATTATTAATCTTTGCCAAAGACCAGCTTTGGCATTACAATACAATTTTGGGGATAGATTAACTAGTGCAGCCCAGCAGGCAGCACCGTGATTTGCAACAATTTGAGCCTCACTCATTCAAGAACCTTCCAGCAACTAGTTCGCCTAGTATATATACCTGCTCTCACCCAGCTGCACTTGTATACACAATCGAACGGCCGAACAAAAACTACGCAGTAGTCGAGAGCTCCAAATTAATAGAATCCGTCACATAAATTCGCCGCAACAAGTTAAACGAGAATAAAAGCGTCAGCATCGATATATATACATACATATATTCAAAAGTCTGTCGAAACTACAGAATACGTTGCGAAAATGCCGGAGAAGCTGTTGAAAATCACCTACCAGGGCGCTGGACCCCAGAAAAAGATAAATGCATACCTGCGGATGCCCTCCCAGAATTACACCATATTGCGTCGCGAAATCGAGCTGTATCTTTTCCAGGAGCGCCAGTTACCCAAATGCGACGTAAGGACCTTCTGGATCGGTGAGTTTTACAATGAAAATGCCGCAGAGTGAATGTTTGCTATACATACAAACAGTTATTCGCAATTGCAATCGAGTCGCAAAGATTGCAACATATGTGCATGGTATAAGTACATTCGTTTCCATACATAACTGCATCCGTCCGAGCAGTGCTGACCATTTAGCTTTTAAGTCTCGTTACTTAAAAACCAGCCCAAATTATTGCGT

AGCAGGGATTCACCTAGATAGTATTCCTGCTCAATGCTTATCATTGGCAAAATTGTGTTGACACATTAGCTGATAAATATGTACATATATATTTTTATGGTACAAACGTGGTCAACATATTAGATGCACTGTTTCATAAATGGCACACATACAAAACATTTTTGATTTTACATATTTCTGAATTCTCGAATTATACATATATATTTTAAATACATCTAACGCGCTTTTTAATTTGTAAAGTATGTGTACATGTACTCATATACATTTACATAGATCAAACGGGTTGTGCTGGTACAACCACAACTGTAGCTAATTTGTCTAACTAGAGCCCTCAGTGATTCACCTTTGATAACAAAACTTCACTGATTCATAATTGATTTTATTTTCCTTTGCCTATTCTAACTTTTGCAGACGCTGATAAAGATGAAATCGAAATAGTCAACCAAAATGACTATGAGATTTTCCTGGCCAAGTGCGAGAGCAATATGCACGTTCAGGTGGCTCCACTGGCGCCCGTAGAGGAGCCAAAGGCCACCAAGCAAGAGGGTTCTTCGGCCAACGCTGAAGCTCCTTCTGTCGACGATCCGAG

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