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CRIMIC CR00724

Gene Atg7

CG5489

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:18622270..18622287	55E2	FBti0199615	79288

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AAGAGATCAATCCGTCTGCGGAAACG	GGATGGAGTGAGGCAAAATTCCTAGC

Images

Construct Design Sequences

sgRNA sequence	ATTCCGCTACCGCATGCCGTTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CGTGTCACTGTGCACTGTTG	CGGTAGCGGAATCTGCAGA	ATTCGTGCTCTGGAGATCCG	CTCACAGATTCGCGCGAAAG

 

Amplicon 5'

Amplicon 3'

CGTGTCACTGTGCACTGTTGATCCAAAGTTCGGTCTTTGAGCGACTGAAAATGGAATAGAACGAAATGCAATGAGTATCTGTATCCAAATTTGTACGTACAACTTTATAAAGCGAGTCTATAAGAGATTTTTTTATACAGAGTTTTTTTCAGTGTGATTTGGAGCACATTCGACGCCGGTGCAGTAGTACGATCAAGAGCTTTTGAAAGCTTTTCTGTGTCCACAAACACATGCTCCGAGCAAACAAAGATGACGCCTTCGGGCGAGAACAAAACAAATAGCAGCGCTTAAAAACCAAAAACACAAGATATAGAAAACTTCGGCGTTTGTATCGGTTACTTGAGCAACCCGCCGCTCCGCCCATCACTGATTAGATAAAGTTTTAACATTTGGGAGAGCCGGAGAACTCCGGACTCTCCCACGGAACTATAAAACACATTTTGCGCTTGATTGTTCAGTACAAAGGCGATAGTTAGTTGAATTGGTAATTGCAGGAAGGAATCATGTTGACCGAATTCGCTGGCAATCTATCGCATCCGCTGGAGTTTGGTCATGTTCTGGAGGTCGTGGCCAAGACCATCGATGGAGCAGCCAGGTAAGTGTTGTCCGGCATTAATGTAAGACCGCTTTTCCGTTTTCTGACTAATTACTTGTCAAATTGGGCTTCTTATCAGTACGTGACTAATCGATTGTGATCATTTGGTTCGGATAGGTTCCACATTAACCTCTGCACGGCCAAGAGCACGGTCGATCCGAACGCAGATATTGGGCTACGCTTCTCATGCTATTTTCGGAACGATGTGATCGTTCGAAATTCCCGGATAAACGGCGCCTGGGGCGAGGAGGAGTCCCATGTCATGGACCCCAACACCCTGCCCAATCCCATCGTCAGTGGCGAGTTCTTTTTGGTCTACATTCTTTGTTGCGAGGACAGCTTTGCCATCTCGATAAATAGCCGAGAGTTCTGCAGATTCCGCTACCG

ATTCGTGCTCTGGAGATCCGTGATCAGATCCAAGTCATTAAACAGGTGGATCATCGTACTGTGTTTCCAAATCCGTGGCCAGCCGTTCACGCCTCGGACTACTTTAAGGCGTTCAGTAACGATCAGCCAATTTTGTTTAGTCCGGGCCATGTGATTGTCCTTACAGCTCGGTGTTTCGAGAATAAAAAGGGACAGTTCATAATCAAGTTCATGGACTCGGATACCAAGCGCGAAGAGCTACACTTCAGTGTGCGTTTCGATGAGAAGGCAGTAGTGCGCAATTCCATGAACAAAAATTTTGAGTAAGTTAACTTTATAATGCCGTACCTCTTTGGGCGTTAACTAATTCTTTTTATAGATTCGGAAGCGAGGAGCGTCATGGAGGTTTCCCCTTCGTCTTTAACCAGCAATTCAAGCTGGCGCTTGCGTTTACGGAACGCGAGGTTTTGACTGCAGTGGACGGTTATAACTTCTTCAGCTACACCTGGCGCACACCTAATGCCATGATGAATCTAGTGGGTTTCAAGGTGACCAGCATAAATGGCCTGGTAGTGCAGATAACTGGTGTGGATCACCTGCAGACGGGGGATCCTACGTGCGCTGGCTTCGAAAAATATTCCCGCCATGATTACGAATGCGTTTAGTAACCATGTTAACCCAACTAATGAAGTCTGAAAATACAAATGTTATCTTAAACTATACAACAAATGGGACTTACATTTCCAGGGGCCGTAACATCGTTACAAAAGTAGCAACCTAATTGATCGCCATTAATGCACTTAAGTCCTTCGATTTCTTGGCCATCGTCGCCTGCTTCCTTACGCGTAGTGCCATGTCGCATTACCAGATAACTGTCAAAGCCTAAAGCTGCGTTGATGACAATCTATAAGGGAAACATTTTAGGTACATCTCTTCTGAAAGGTGAGTATATGATTGTTCCCACTTTTTCCTTTGCCGCTCCAAGCAGGGTGGGCAGCCAGCGGCTTTCGCGCGAATCTGTGAG

 

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