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CRIMIC CR00725

Gene jef

CG12858

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:14743022..14743022	51C1	FBti0199616	79289

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AACAATGAATCCCTACACTGCAGGCG	ACATGATTGATGACAGAGGCCACACC

Images

Construct Design Sequences

sgRNA sequence	AAGGATGTGCCAGTAAAGATAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AGTGGGTCAACACGGTCATC	ATAGGGAAGGATGTGCCAGT	TTACTGGCACATCCTTCCCT	ACTCAACGCACAAAGTTCGC

 

Amplicon 5'

Amplicon 3'

AGTGGGTCAACACGGTCATCCACTCTGGCAACTCCTTGGCCGTCTGGGCGAACACCTTGGACTCGGCACCAATATGCGGCTGTGGCTGATGGCCAGTGGCAAGGAAGCTACTGACGCCACCGGCACAGGCACCCGAGGCGGCACTGCCGCTACCAACTGCCAGCGATGGCAGATTTAGCTGGGCCGCCAACTGGTTCATCGACTCCTCCTCCGCCCGCTTATTGGGATCCACGAACTGCGCCTGCTGCTGTGCCGCCAACTGTTCATCGATGGGATCGTACTTGAAGGTGATCTTCGATGCGGAGATTATCGCACAGGTCATCAGCACCGAAAAGATGGAGAAGCAGATGTTGTAGTTCTTCTCCTTGTTGCCGGCACCACAGGGATGATTCGAGAACGAGGTGGAGTGATCCAGAGCGATGCCCACCAGGAACATAGAGATGCCCCAGCCGAGTGACCCAAACATTCGCTGGTGACCGTACTTGTCCGCATCCTCGCCCAGCAAAGTTATTACGGCGGAATCAGCCAAGGTAATCGCCGGAGCGCTGAAGAACTCCCCAATGAGGATCACCAGGAGCAGCAGGAAGAACGCCTTTTGGATGTCCTGTGGAAGGAGATAAATTTGTATAAGTACTTTTAAAAGCATTCCAGAGATACAATCCTCTTGGCAGCTGAGACGTTAAGTTAAAGAGGCGAGAACTTTTAGACATAAATTACTTAGTAGATCTTGAAATGGCAATAAAGATATGGATTGCAAAGCTAAAACTATATAAGTTAGGATTTCATATCCAACATTACATTATCAAGTTTGTATATCATAAATGGAACACAACCTAAATCGAATATATCAAATTTAACTATTTGGAAACGAACCTAATAAACATTACATTCTAATATATACAAATATAAAATTTATTTCATTCCTATCTTTTTGAAGATCTTATCTTCCTTCCTATCTTTACTGGCACATCCTTCCCTAT

TTACTGGCACATCCTTCCCTATTCACTTACCGGAGTCCTGTACACCATGGAGCTAAAGATGGGCGTCACATAGTCCCTGTGGTACTTGTCGTCGTAGTTGCTCACAAAGTTGATGTGCACTGGTGAGATGCCCGCATCGATCCTTGGCAGCAGCAGTGATCTCTTGTGCCGACTGTGTGCTGCCTCCGCCTCCTCCAGCGCCTCATCGGCTGGCATGGTGCCAGTGCCCATATCCAAGTGCTCCTGCTCGTACATGGCCGACAGGTCGCGCTTCGTGCGTGTGTAGGTCAGCACAAAGTCCGTGGCATTGCGTCGCTCAATGCAATTGATGGCCTCCGGTCGGATGAAGCTCAGCGGAATGGTAAAGAGTACCCAACAGGCCAGGGAGCCCAGGAGCAGACGCTTGCCCTGCCGACAACGATCCGCATAGGAGCCCCAAAACGGGGCCGACAGGAACTCCACGAATGGCCGCATTCCCACCAGAATTCCGCACTGGCCCGGATTCATGCCCATCTGCTTGAAATACACGCCCATCAGCGGAAACAGTGATCCAAATGCAGCGTAGAAGAAAAAGTAGAAGCTTTTCACGGGTATCAGTTCCCGTTCTGTGCTCGCCCCGAAAAACATTTCCAGGATGTCCGAGTGGCCGCGAACTTTGTGCGTTGAGT

 

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