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CRIMIC CR00728

Gene CG5142

CG5142

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:13108804..13108814	34A5	FBti0199617	80363

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GATCCTCTACTCCAGCGAAGATTTCG	CGGAATTGAGCTGCGACATAAACACG

Images

Construct Design Sequences

sgRNA sequence	AATCCCTCTCACAAAAGTACCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCGCAATCATCCAGAGCTTG	TGTGAGAGGGATTCTGCTCTC	GGTATCTAACAGTCGGTACAGCC	CACTTCGGCCAGAGTCCTTT

 

Amplicon 5'

Amplicon 3'

GCGCAATCATCCAGAGCTTGGAATAGGCGCCCAGATGGATATTCCCGATGGCGGTGCCAGGAGCGTGGGCAATCCCATCACCATGGCCATTTCGGGCATAACTCAGGCACTTAATCTCAAGGCAGCCATTGAATTTCAAGATGGCAATGAGGAAGCAGCCAGGGACGCCCTCCTGGACCTTCCTCCACGAGCCGAAAGCGAACTAGATCCGGTCACATTGCACAACATGGCCTTAACCGATGTCGAAGGTCCTGTGGCGGGTCTGAGGAAGCTGGCGTTTCTCCTGGAACTGGGTGCACCCTCCTGCCCCAAGGAGACGTTCGCCAACATTCTGCTCATCTGCTGCAAGAACGAACTTTACGAAACTGCTGCGGATATCCTGGCGGAGCACACGGACCTCACCTACAAGTATCTTTCGCAATATCTCTATGAGTTATTGGACTCCCTAATAACCGCACAAACAAGTGCCGAGTTGGCCGAGAAGAAACTAGGTACTCTGGCCTCCAGTCTTGCTGGTAAACTGCGCAGCTTGGCCGCAAAAGTGCAGGAGGTCAGGGCCACCAATGAGCAACAGGCACTCAGGGATGCCCTCAAGGACTACGAACAGGCACTGGAACTGTGAGTTAACCTTAACGTGATATTTATTTTCTAGAATTTATTTACATTTCATTAAGTATGGGTCGCGTCTGGATTTGTGTTTATCAAATGCAACAGTATTTAATTTTATAATATTACGCTTTTTCAAACAAAGTTACTTGCCTGTGGTGATGGCTCGAGCCTGGATCTCTTGGCGGGATGATGACTTCGTCGGAGCTGAAAGGGAATTCCATGCCAGTGCCGAGTTCTGTTCGGAGAACTCCATTTGGCGATTGAATGCAGGACATGTGCTCTTCATGCAGGGCGACAAATACAACGAAGCGGCTGCGTTTTATGAACCCATCGTGCGCCAGCATTCGGATGATGTGAGATTGAAACATTTAAGCTATTCACACAGATATTTACTTTTTGAATGCCCGGTACTCAAAGTGAATAAGGGAGTTATACCAATAGCTAAGAGGTGTTTGAAATATGGCAATATCTTAGTAAGGGAAATATTTATAAAATCCATAAATCTTAGTAGAGAGCAGAATCCCTCTCACA

GGTATCTAACAGTCGGTACAGCCGACTTTATCATATCAGGTGGCACCCAATTTTAATCTACCATCCCCCGTTTCAACCAGATCATGTCCGTTTCTGCAGCGGTGCTGGCCAACCTGTGCGTCTCCTACATCATGACATTCCAGAACGAGGAGGCCGAGGAGCTGATGCGTAAAGTGGAAAAGGCCGAGGAGATGAAGGGCAATCTGGGCAAGCAATACCACCACCTGTGCATTGTCAATCTGGTGGTGGGCACACTGTACTGCGCCAAATCCAACTATGAGTTCGGCCTATCCCGCATCGCCCATGCCCTGGAGAGTGGGTCTGGGAACCGGCTGTATGCCGACACCTGGCTGCATGTGAAGCACTGTATCCTGGGGCTTCTCACCGGCATGGCCAAGCAGAACATTATCCTGCCCTATGCCACCGTGCAGGAGGTCCTCAACTTTCTGCGCTTTTGCGAGTCCTACGGCCTCTTTACGCCGGCCAACATTTTTTCGGCCACCGAACAAGTGCCGGAGGAGCCACTTACCATTGGCCTGGAGGCCAGGAAGTTGAGGCTCCTATTGATTAAGCTGAGCGAATACGATAACTTTTAGATTCGGCTTTAACTGTTTAACAAATGTATAAGAAATAAACATCAAATAAAATTAAGAAGAAGTTTTGATATATGTCATATCCTTTAATTGCACTTTCCTGCACCTTCTTTAAAGAACTTAATTTAATTGTGATAAGACAAATATGAAATTTCAGAAAAAACCTTATCGATTAATTTGTCTTTGCTCTGTGACAGCTGGTAATGCCACAAAGGACTCTGGCCGAAGTG

 

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