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CRIMIC CR00729

Gene crol

CG14938

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:11800867..11800881	33A1	FBti0199696	79290

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTCATCGTGCGTCCTACGTCTTCTTC	AAGAGCTGCGTGTTGCAATCGTGTGT

Images

Construct Design Sequences

sgRNA sequence	TGAGTAGAAGCAGAGTTGACAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AAAGCTCTTGCCGCAGATCT	AGAGTTGACAGGCACACACA	GCTGATCCTCAGGATGCTGT	CTCGCTCATACCGCTCCATT

 

Amplicon 5'

Amplicon 3'

AAAGCTCTTGCCGCAGATCTCGCAACGGAACGGCGTCTCGTTGGTATGGGATCGCATATGATTGGCTAGGTGCTCCTTGCGCGTATATTTCTTGCCGCAGACATCGCACTGGTGCGGCGTTTGACCTGGATATGTGGGAGAGGGTAGCAGAACAGAACGGAATTGTTGTATATAGGGAATGGCGTTTGATCTCGTTCGACAAGACAAAATCGCATTCGTAAGCGCAAGCATTTTCTTTTTTCTCGCTTAACTCGAATCAGCGGTCATTTCTCCAAGAGGGATCCTTGCGGCAGCCCATTGGAGAAATGTTGGCGAATCGGAGAAAAAACAAAATTTCAGATCCCCCGACCCATCCTCGCGCGCACCGCCGCTCAGTCTTATTTTTTTGTTGTCTTTTTTTTTTTTTTTAGTTGTTTAGTGATGGGGTGGCTGCGTGGGTGGGTGGGTTTATTTCTTTCGTATTAAAATCTTTTCTTCGTTTTTTTTTCTGTTTGGTGTGAAACTGGCCGTGGCTTGCGTTGTTGTGGTTTCGAACGAACGAAGCGAACGGAGGCTGCTGGCTCGGGCATATGGACGTGGGCGTGGTGGTGTCAGATGTGTTGTGGATTGTATGTGGTGTGGATGATGTTCATTGGATGTGTGTCTTGTTTTGTGTGTGCCTGTCAACTCT

GCTGATCCTCAGGATGCTGTTCTTGTTACGTTTTTTTTTTTTTGTGCGGAACGTGCAGTTCCAGAAGAAAGTTTCGCCAAAGAGCTAAAGTTTAAGGTAGGTATCTAAGCCTACCTAGCAGATATTTATGCAGGTGAGCTGATTGGTGTGTTTGTGTGTGCGTGTTTCTTGTGTTGTGTGTGATCCAATTGCTTTCGTGTGATCGAGTGTGAAATGTCGCAGTTACACAGAGACGTTGGCAATCGACAAGCAGCAACAACGAACTTTTCTTAGCTTTTCTTTACTTTCTTGACTTTTCTTTGCTTTGCTTTGGACACCCTGTTCGACAGCAGAGAGAGCGTAGACAAAGTTGCACAGGCATTAGATATCGAATCGAAACGGGAGTAAAGGATTGGACGTGGACTTGGAGTGATGGAGTGGAGCGAGCACTGGATGAAGTGTTTGGATTGGTGGTGCATGTGAGCATGATTAAGACTTGGTGAAGCTGGGGATGTGTGATGCAGAGATCATGGTTGGGGGGAAATGGAGCGGTATGAGCGAG

 

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