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CRIMIC CR00732

Gene Rbcn-3A

CG3585

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	X:6253135..6253161	5E3	FBti0199618	79291

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCAACCCACGTCAATGGCAAATTGGA	TCTCGCCAATCTCGAACTTGACACCT

Images

Construct Design Sequences

sgRNA sequence	CCTCCCTAGCCCACTCGAACAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CCCCACTGACGACATCACAA	CTGGCCACGATAATGAGCA	GCTAGGGAGGGGGTACACTA	GTTGACACAGTCCCCCGTTC

 

Amplicon 5'

Amplicon 3'

CCCCACTGACGACATCACAAAACGTAAAAAACAAAAACCCATTGCAAGTAATTTGCATTTGTGATTCATCTCCGCCCACGCAAATCGATAGGTGGCCACACAGAGACACATAGAAATGGGGTGTTGGGCAGCTATTGGCTTACCCGTGTGCTCCTCCTCCTGCTGGTGAGTGGGCGACTTGCTCTTCCACAGCTGGATGTTGGTGCCGCCAGTGAGGAGGCGGGTGCCCTCCAGATTCCAGGACAGGGTCACCACGTTCGAGTGGCTGGTGAAGGAGCCCGTTTGCACCCATCTATGGGGGTATGAAAAAATAAGTTAGTATGTCTTTGGGCTTCTTAATACCATCGCTAGCTTACCTGTAATCCAGAAAGTGGGTATTGTGTTTGCTCGACTCAATCACCGGCGTGGGCTCAAAAATGCATATCTTATTCTCATAGGCTGCTGCTATTTTTCCGGTATCGGTGCTGCAGTCCAGGGCGGATATCTTGACATAGCCATGGTTGGCGCCCGGAATGATCTGGACGCGCTCGAAAGTGCTTGCCAGGATGACCACATTGCAGCCGGCGGCGTAGGCCTGATAATAGGAGGGGCGAAATGGGGATTTTGTGAGTACTGCAGTTAATCTAAGCGTTATACGATCGGTATGTGTTTTCTCAGTTATTTGTTTTTGAGTGGCTATGTGTATATAGGGCGGGTGGGTATATAGCTTTATATCGATAACAATACCCCAACTGTCCAGTTAACTGGCTTATCAGGCTGGCTGATGACAAGGCTCTTGCCACATCATGTTTGGTTACCTGGTTAGCTAAAGTGTGTTTGATTTATTTGTTGAGCTGTCGCTGATATGCTCATTATCGTGGCCAG

GCTAGGGAGGGGGTACACTATTTATAGTGGAAACACTATTCATGTTAATTATCTCTAAAACATTACCGCATGACGATCTGTGGCAAAGGATCATTAGTGCGGGTGTAAAGGGTTGAGTCAGCAGGCTTTATCTTTATAATTGGCTCATTGATGCGGGAGATTACAGATACCCCGTTTAGTTACAATAGCGATAAATGGGGAATATCAACAGAGAATCTGTTTGCAATGGCTTATCGCGATATTGGAATGTGAGCCTTATTTAGCTAGTTAATGAATAAAAACATCATTTCATAAAAACAAGGGTATTAACAACTTCGTCACTACACACTACACACCTGCTAACTTTTTGACATAAAAAGGTTCAACTACTCTGCTTTTCTGTCGTGTTTATATTTAGCTTTTTGCTTTCCGGTTTTCCCATTGCTTTAAATGTCAGCTTATGATGGGTTTATGCGTTTAACTGAGAATATTACCCATTAAGTAAACACACCGATGATACTTTTCAATGGAGGGTTTTCCGAACTTTTCGCTTACCAACCCATTAGTGTTCCCGCGGATAAGATTACTTTATATAAAACTTTGTTACCTGCTGAAAATAAACAGTGATTTTTTTACGGTATCCTATTGTAGTATTGCTATACTATTAAAATTTGACTTTAAATGCGGTTTTAATGAGTTTATTATTTTTGGTTTCCCCTTCCAAACGCTAGGTGGCGCCAGGGGAGTGTGTTCGTGCCGATCATATACTAAATAAATATATGCATACACACATACATCCACACATATGCAAACACTCGAAATTAAACAAATTGAAATGATGGCAAATGATATGGCGCAAGGCGGTGGGCAGTGGGTGGGTGTTGGGTGGTTGGTGGTCGGTCTGTGAACGGGGGACTGTGTCAAC

 

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