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CRIMIC CR00733

Gene betaggt-I

CG3469

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:4946311..4946332	25B4	FBti0200645	80631

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	no band	AATTGGTTCCACTAGCACCTTGCGAC	AAGTATCCACTGGCTTATTGGGGCGT

Images

Construct Design Sequences

sgRNA sequence	CCTGGCCACGTATGGAGATAAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GAGCCGCTGTTCACAAGTTG	TGATTGGGAGTGGATGTGGG	GGCCAGGTAGCAGACACTG	ATTCCGCATGCACTCCAAGA

 

Amplicon 5'

Amplicon 3'

GAGCCGCTGTTCACAAGTTGTTCAATGAAAAAGACTCTTCCTGTTACCTGTTTTTTGCTGCGCTCGATGTATTCCTTGGGCAGATTGGAAAAGTTCTTCAACTTGCTGGCCAAATATTGCGTTAAACGCATTTTGAAAGGTTAATTCAATTATTAATAATAAGAACTAGTAAATTAAATGCGGCTTGCAATCAGCTGTTGAGAACTGAAGAATAGCCATTCACAGGAAGGCCCAACAATTTTATTTATATTTAATGTTTATGCGACTCTAAAGATTATGAACACTTGATGTAGGCTTGTAAAATATTTAAATTAATTATTTTCGAGGTTTAAGAAAATATCTTAGCTTATATATTGTTAAACATTATGATATAGAAATTTAAGAACTAGCAATTATTTCCCCGTGTAAACGTGGTTCGCTGTGACTGGCAAGAAGAGGTAAGAAGAAGCAGCTGCTATTTGTGGACTACTCTTGGTGTTTTATATCGTTCGCCGTAGCCCTAAATTGCAATGCAAACCGGCTTTATATAAACAAAAGGTAAGTGCTAGACCATATAAATGTGGGCACGTGCGGGCGCAACATCATTGGAAGACGGAATCGCAACCGGAGAGCGTCTATTCCGGCCAGCTCAGTCCAACCGTTTGGGAAGGGCCTTTCAACGCTTAGTCATATCCATATCTCAGCGCACACCGAGACAATCCAGTTCCTACCCACCCATAAGTTCCTCATTTAACAAGCAATATGTCCCACCACATAGATATGGAAGACCGCACTGATACGGAGCCCGTGCTGCTCTCCAAGCACGCCAAGAATCTGCTCCGATTCCTTAATCTCCTGCCAGCTCGCATGGCCTCGCACGATAACACCAGGTAAGTGCATCCTGGCCTCGTATTTAACTTTTGGTTAACCATTTCCTTGTCCATCAGGAGCACCATTGTGTTCTTCGCCGTCTGCGGCCTGGATGTGCTAAACTCATTGCATCTGGTGCCGCCACAACTGAGGCAGGACATCATTGACTGGATCTACGGAGGATTGGTAGTGCCACGCGACAACGAGAAAAACTGCGGCGGTTTCATGGTGAGCATAAATTCGCGTCTGTCAGGTGTACAAACAGTCGTTGACAAGCCAAACAGAACCATCTTCCGCTCCCACATCCACTCCCAATCA

GGCCAGGTAGCAGACACTGCGGCAGACGTTGTCGCATCCCTTACATATTGGTGTTTATTCAGTCAGTTGATATACGAGCATTCGCATTTCCTTCATTGAGAAGCATCCACGAAGTAAATATTTTGAAAGATTCATTTCCAACAAAAAAAGTATGCAATTCTTTATTAGCATTTATGAAGTTTGTTTTTGTTAAAAATATCTAAGAAGCCACGTCTTGTTTCCCCATCATGCATCACATATTTCATGATTCCAGACCCTTTAGTTTAAATTACAAATCTAAATTTTGTTTTTAAATAATTGGATTCTAATGAAAACCCATTAAATTGTTAATTCCAAGGTCAACGCATCTGCTTTACATAAAAAGCAAAACGGCATTACTTCTGTTACGATCTATACTGTTTAAATATGCATAATTGTAGAGGAGTTCTCCATTTGCGCAGCAGCTCGATTTACCATTACCCACCACACCCCACCCACCGACACCTTGAATTTATCGCGCCACGCTTTATCAGCGACGCCAGCAACATGCAAAACCACTTTAAAATAGATTGATTAATTAATTGGTATCTTTGCAGGGCTGCCGCGCCATGGTGCCCAAAACCGAGGACGCCGAGATCTTGGAGTGCATGCGGAAT

 

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