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CRIMIC CR00734

Gene Golgin245

CG3493

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:23376709..23376726	59D9	FBti0200646	80632

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CACGGACGGATGAACTCGAGACATTT	GGCCTGAAGCTTTTTGAACTGCTCCT

Images

Construct Design Sequences

sgRNA sequence	TGTGTCGGTGAACTGGGAAGGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CTGTTCGGACTCCAGCTGTT	CGATTGTGCGATTGTGACCC	AGTTCACCGACACACATGCA	ATGGCCGCTTCGTTACAGAA

 

Amplicon 5'

Amplicon 3'

CTGTTCGGACTCCAGCTGTTGGCCGCTTCTCTCCAGAGCCGCCGTAATATTCTCAGGATTTTCGCCCAGCAGCTGGAGCTTCGTCTGATAAGCCTGCATTTTGCAGCTCATGTCGTCCATCTCAACGCGCAGAGCCTCCTCCAAATGCGCCTTGGCCTGCTGCTCCAGCGTGCACTGCTCCCTCAGCTCGCTGATTCGGCGCAGCGCCTTGTCTTGCGTCTCCACCAGCACAGACTGCAAAGCGATATTATCGGAAATGTTCGTTACACAAGCTTATCAAGCAGACCGATTAGATAGGCAGATAGATAGATACCCGCGCCTTGGAGCTGTCGCGTTCCAGTTCCTTGTACTTTTTGGCCAGATCCGTGTACCGACAGCGGTACTTGTGGTAACGATCAAGTGACTTCTTGTACGCATCGTACAGCTGGTCCTTGGTGATCACATCCAGACGAGCCGTTGAGGCGGAGTCGTCGAACTCGCTGCTCGTTTCGTCCAGATCAGATTGCAAGTGGTACACCTGGAAAGTAGAACACATAATTAAAGCAATCTCGGAGACTTTTCGGCCACTTACAGCTGGCGCTTCATATGTAGGCAGGCGGAAAGACACGTCGCTGGCCATGGACGAGTTGGAGAGCTTGCGGGTCCTGGAGGTGGCGCCGTTCAGGGATTGCGTCGATCGTCCGCGCAGAGCGGATGCACTTGTGGCCGGCAGCTTTTGGATGCGATAAGGCGAGTTCTGAGGTGTGTCTGCGCGGTGAAAGATGGATTGATATGTGAGATAAGGAACCCCAGGTGACTCATGGCGTACTCACCCTCCTCCGTGATGCTGAAGAAGTTCTCATTGTTGCTGGTGTTCGTTTCCGAATTGGGTGTTGTGCTCGACGATGAGCTCACGGCGGCCTGGGAAAAAAGTCCAAGGAAGACTCATGAGCAGCTGGTCACTTCACTTTTCACTTGTGCACCCAGAAATGGGTCACAATCGCACAATCG

AGTTCACCGACACACATGCAATTCCGTGTCTGTGTGCGTATGTTTTCCTCAATTTGCATTTTTCATCGGCAATAAGCACCGCCCCGCCCCCGCACACAAAAATACCTGTGCAGCATTCGCAAATTGCTGGAACTTCGAGGGCGACGCCTTCACCTCCTCGATCAGCTTGTTCTTCAGGTTGGCGAACATTTCGAGTGCCTGCTGCGCTCTTTTTCAACTGGTTGCGCTACCTGGTTTAAGCACTATTCAATTTGTAATTAAAAAACATCTAAATAAATACGTGTCTGCAGATGTTCATTCAACGGTCACACTGCCGATAGCCTCGGGAGTTATCGATAACAAGCGCAGTATTCATTAGGGACGAACGCAACTGCGGGTCATTAAGTTTATGAACGTGACGAAAAATTTTAATAAACATAAATAAATGTTACGTACAATGTAATTTTTATTTAATACACACAAAAGTAACAATTTAATTAGTTATAATTGATAATACCTACCTCCATGTTTTTTAATACCATCTTGGTTCAAGATATTTTCCACAGGTGGCGCAGGTTATCTTTAAAAATATTTGGAAGCCTGTTGAACGCCCTTTCAATTCTTTAAATATAGATATATAGAAAGTTCCTAGGTATGATGTATTCAATTTTTTCTAACAATACTACTATAATGAACTGTTTACACGCTAAAACTGAATGAAAGCTGGTCAAACTTGCTAATTATGCAATGATTTAGCATTTTAAGTAGCCGCTCGTGTTTGAGGAATCCTCATGTTGTGGGCAAAAACGCTTAGTTAAGCACCCTACAGTTTGTTTTTATTCGAAGATAATGCAGCATACGTTTAAATTGCACTTCTTTATTTATAAAAACCGCGCAATAGTTCGTATAACTGTGTTTTCTTGTACGTGAAATATACACTCTTTTCATAAATTCTATTTTTTACAAGTTGTTATTCTTTTTAAGTTAATTGATTTTTAGTCGATTATAACGGCAGGCGATAAGGCCTTAGTTATTAACGAAGAGGAATTGAAAAATAACTTATAAAGAAACACATCAACGAACTTTATTGCTTTGGTTCTGTAACGAAGCGGCCAT

 

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