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CRIMIC CR00737

Gene HLH54F

CG5005

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:17759149..17759166	54E8	FBti0200648	83187

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTTCTGATTTTCCAGGCTCCCAACCG	TTAAATGCCATGGGCATGGCTAGTGG

Images

Construct Design Sequences

sgRNA sequence	CGGTGTTCTAGTGGGGGGCCCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CGTTTGAAGACCAAGCTGCC	GACTCCGGTCATGGGATGTG	CCCCCACTAGAACACCGAAA	CAATCCATACGGGCGGATGA

 

Amplicon 5'

Amplicon 3'

CGTTTGAAGACCAAGCTGCCCAACATTCCGCCGGACACGAAGCTCTCCAAGTTGGACACGTTGCGTTTGGCCACGCTCTACATCAAGCAGCTAATTACGGCTGTGGAGACAGGCAGCCACTCGCAGAACCACCCGCACAACCACAACCAGCACCACAGCCTCAACCACAGTCACTCGAGTACCACCAGTTCCGAAGGCCTGGACACAAGTCACATGGCAGATTCGTCCGGTGGAGGCAATTATAATTTCCACAACAACGGACACGGCATGGTAAGTGTCATGCAATTGCGATCCCCCTGCCCGAATATAAGTGATGTCACTGCCACCACTTAACGATGCACTGCACCCGCGGCTCGGGGGAACGGGAACGCATTAATGAGCCGGCCACTTCAGGTGGATCTCGTCAGTTAATGACAGGTGTCGCAGGGGATGCCCAGTGATATGCCCAAGATCAATGTTTATTCATGGATTTCAGTCTAATTGAGAAGTAATGTTCAGCCGATCGGGATTCCCATAAGTTTATGTCTTTTTATGGAAAGTTTGGTATTGGGAATGAAAATTTTTCTTTTATGCGTTTTCTTTGAAAAGCCATTAAGGAAATAAAGTAGGTAGATATTTGTAAATTTTGCTAGTTGAAATTAGCCTTTCGTAGAATTTTAACTTAGAGATCGTGGAAAGTTACACGGAAACCCAGGCTTCAGTGTTTAAAATTTAAAAAATTGATACTATTACAATTTAAACGTATACGCAATCTATTATTCTCATTTTTTTTTTAACAAGCAACTTAAGTATTTAGTAATACTACTCAACTTATAGGGGTAATTCAACGCAATTTTAGCGAAATATAGATCAAAAATCCAAAGGAATGTGGTGCAACATCTGCTTGGTGATGCGGACGCGATGCGGCGACAAGTATTTCCACCTTTTCTAGATGTGGAGGTGAACCACAAACGTAACCACATCCACATCCCATGACCGGAGTC

CCCCCACTAGAACACCGAAATCACATTTTGTGATTTCAGACTGCCTTGCTTTCCTGTAATCCGATACTTTTGTGAATTTCGAAAATATTAAATATGAGTGACAGGCTGTTGGTGGAGCGGGCAGGGAACGGAGATGGTAACGCAGACCTGCGGATCTGGATGGGGGGCACTAGTTAGTCTTTGCTTTTATTTTTTATTTTTAAAGATGCTCTTGATGCTTTGAAGCGACACAGTGAATGACATGCGAGGGAAAGTGTCATCCTCAGTAGTTTTCCCACTCGGATTATTGTAATTAAAATTTGTCTAACCGTGTTTATCCATCAGCCATTCCCGCTGGAGTTTACACAATCTATCACTCAAGATTCATGGCGTGCACAACTTATCTAAATAGATGCAATTGTTTACACACCGAATGCGTGAAAAATTCAGCAAATACGGCTCCGTCTTGTGCCCATATGGTGGCAAATCAGATCGGATCGGAGAGTCCCGACCCTATCCGAATTTATACCATCCACGCGCCCAAGATGATCTGTTGGGAACAACCCATTGGTTCGCTATTTGCTCGCTAGCCCTAGCATCCCTATATCCCATTTCCAATCCCTCATTGTATGGCATACAAATGAGTGTCTCTTGTCCGCAAGTGTTTGGCTGTTTATGTTTTTATAGATCCGTTAATGAGGCTGTTATTGTAGATCTAGACGTGGAGGCGGGGGTGGGCACTAAAAACAATCAAAGTTGCGTAAACAAACAATGGCGCTGTCTCGGGATGGGTTGTCGTCATATTGTTCCATATATAGCCCCTGCGGTTTCCACAACTCCTATGATTTATGCCTGTTTTATCCACACATTCCACATTATACTGTCTTGCAGAGCTGGCCCTTTGAGTTCCACCAGAGTAGCCGGAGTTTGGCATTTGCCCCATCATCATCAACCACCTCATCCGCCCGTATGGATTG

 

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