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CRIMIC CR00738

Gene NC2alpha

CG10318

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:21670408..21670428	57F7	FBti0200649	80634

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTCTTCATTTCCAAAGCGGTCTTGCG	CCAGAGGCGGCTTTTTTATGGTGTCT

Images

Construct Design Sequences

sgRNA sequence	TACACGCCTCGCATAATCAGAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCTTGTGAGGCGGAACTTTG	TGCGAGGCGTGTATGTACAT	AGTAGAGGAGAAACCAAACCGG	TTTGGAATGATGGGAGCGCT

 

Amplicon 5'

Amplicon 3'

GCTTGTGAGGCGGAACTTTGTTCTCCGGCGGTGGTAGGGGATCGGCGATAAAGCAGTCAAACAGGGGCTTGGAGAAGAAGATCAGAAGTGAGCCTGTGATGAAGGAGATCTGGAACAGGCGTGGATTTCGCTGAATCCACGTGCGTTTGGGTAGCACAGCGGTTCGCCTGCGCTGATTTGCTTGCTGTTGCTCCTTTTGGTCCAGCAAGTCTTGAATTTCCTTCGGAGGCCTATACTGCGGTATGGACATTATTTGTTCCTGCAAAACGAGTTGATAATAGTGTATATGGAATAGAGAAGTTGGCTACAACTCACGACTTGCCGGTTGCGTTCTTTTCCTTGGATGCGTCTGGTAGATAAGTCTTCTCCAAATCGATTCCACTGCAGCAAAGCGATTCCTTGTGCCACTTTAAACAGCACTGGCGCATTGCCTCAAGGACATCCAAGCACTTGTCCTCCTGGTAGTGATTCTCTGTAACCGAGTGCAAGTCAGCTTACATAACCATGAAAATGGAGTCACCGGCGGTGCTTACTGGTCAGACAGGCTTGTATGCGGCAGGCGTTCGGTTTGCAGGGATCCTTGCGAGTGTCAGACATGTAATCCTGCCCAGAATTCTTACGATATTAAATCGCTCTTGCAATATGTGAAAAGTACTTGGAAGTGTTCTGCACTCTTCTTCTTCTTGGCGGTTGTAGTCATTCACAGTGCACGATCAAGGTAGCCAGACTGCGCGACGGTCAGCTGTGTAGGAAACCTAATACATCAAAGCAAAATAAAAAACAATCACATAAATAACAACAAAAAGAAATAACATCTCTTATAATGTACAAAAATTTTTAATCTTTCAAATTTTGATATTAATACCTTTTGCTTCCCTGTGAATACCCCGCCTGCTGCGAGAGCAACTGCCATTCACCCATCGACGCCGACAGTTGGGCACCAAGGAACTGTTCTTGTGTGAAAAACACACACACATTTGTTTTATTTCACTAGGACTCTTGACGATTACTTCACAAATCGGAAAATGCCATCGAAAAAGAAAAAATACAACGCACGCTTTCCGGCGGTAAGTTGAAACCACCGATTGGCGACAGGAAAGCTCCAGCCATTTGGCGGCCGTGAACGCCGAAGATGGGTTATCAGTTTTCGTGCATGTGTACATACATATGTACATACACGCCTCGCA

AGTAGAGGAGAAACCAAACCGGTGCGAGATTATAATGTTTCCAAACCATTACCATAAACTCCTTGCAGGGTCGCATCAAGAAGATCATGCAAAGCGACGAGGAAATCGGGAAGGTGGCCCAGGCGGTGCCTGTTATCATCTCACGCACGCTGGAGCTCTTCGTGGAGTCGCTGCTGACCAAGACGCTGCGCATCACGAATGCGCGCAACGCCAAGACGCTATCGCCATCGCACATGAGGCAGTGTATCGTATCCGAGAAACGCTTTGACTTCCTCAAGGAGCTGGTCCGCAACATACCCGACATCAGTGTGGCCGAGGAGGCCGCCTACAACGAGGACGATGTGTTACGCAGCTCGCCAGAGGAACAGTATCCAGATTCAGACACGCCATATGATCTTAGCCTGCCATCGACGTCAATGCGCTCGCAGGCGAACGGCACAGCTGCCTACATGCGCTCCATGAGTCTGAACAACGGAGCCGGATCGGGCGGAGGAGCCGCAGCCACCAAGCGGCAGTTTCAGTCGCAGCACTCCACCCAGGAAACTCCCACCACCAGCACCACCCTGCCCGCCAAGCTGGCCCGCTCCGGGTCCATGCCGGCCTACACGCCACGGGGCAGACCGCCCAATCACCTCAAGAAACAGTCGGTGCAGCTCGATACTGCCATACCCGCCCCGATCTGCAACTACGAACTCAACAAGCCCATTGTCAAGATCGACTACAGCCACGTGCAAATGCCGACGGCGAACCTGTGCACACCGGACGCCTCTGATTCTGGGTCAGGATCGGGCGCCGGATCTGGAGCTTTTAACTTCGACATAGCTGCTCCGGTGATCAATATCGATCTGACGAATATCGTGGCCGGTGGAGCACCTGGCAGTGGTGTGCCCCCCGCCTCTATTGGATTACCAGCGGCCACGCCGGGGGACAACAATAATGTGAACCAAATGGCTGGAAAGAGCAGCGCTCCCATCATTCCAAA

 

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