• About
• Search
• MiMIC
  • Technique
  • Constructs
  • RMCE Lines
• Transposons
  • GDP
  • Others
• CRIMIC
  • Technique
  • Constructs
• Order
• Downloads

CRIMIC CR00739

Gene CG5149

CG5149

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:7387837..7387852	27F3	FBti0199619	79292

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTGCACTCCAGGATGTTAACCAAGAC	GTGTACATAAACAAAATGCGCGCCGG

Images

Construct Design Sequences

sgRNA sequence	TCGGCAAAAAAAGAGAAATCGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TTGGCCCTAATTGACGACCC	AATCGGGACTTTCGAGTGCA	GAAGAAAGTTCACTCCGCGC	GCGTTCCTCCTCATCCGTAG

 

Amplicon 5'

Amplicon 3'

TTGGCCCTAATTGACGACCCCAGTCATTGAAATCACGACTAATTCGTTAGCAAGCAAATTCAACAAAGCAAAAGACAAACAGTGAAATGCGGCAGCAAACAAATTAGACAAATTATGGACACGTTCGCACTGGCCCAGGAATTATCAATTCGAAACTGATAACGCCCGAGTTGAAGATATCGAATTTTGCTATACCCTAGGAAGCGCTGTGATAATCAAACACGAGCTATAAATAGATGGAAATATTATAAGGAAAAATCATCATCTGGTTACAAACAAACATTTTTTGAGTGGTTGCATTTCCGTCATCTGTGCTGTGATAAAAATAAAACACAAATCTGGGGCCTTGAACCCGCATCGGAAAAATTATAGCATAAATTGTGTATGATCAGATTGATTTAACTAAATGGGTTAAAATAACAATTTTGGAAAGTTACGTGTACATTTAATCTAAATAGGACTGACTAGTTTAGAAAATTACATTTTTAAACAAGTTGAATAATTTACTTTTTATAAACTCTGTTAAATTTCCTGGTTTGCACTTTAAATCGAAAAAGAACTGTATTAAAATAAGGTTTAGAAACTTTGAACTTTTGTATAATGCCGGATAATGTTGCGACTCACTCTCTTATATAACTAAGCTTCTTTTTCGGAACATAGTACTATGATTAACTTCAATGTTTAATCCTCGAAATAGGAGTTAGTGACAACAACAAATTAATGCCCTAAAGGATATACCCATTCAAGTGAAGGGTGGCCATCGCAGCAGGTGGATGGACGGCCGTTTGTCATGCACTCGAAAGTCCCGATT

GAAGAAAGTTCACTCCGCGCGTCCTTGAACTTGTGACCCTGACCCTTCCGCAAATGCAGAATGCAGTTGCCATTGATTTTCGCAGTGCACACGTGGCACGGTAACTGGATTTACTTGACTTACCACCAATCTGTCTTGGGTCAACTTCTCAAGTGCTCCGCCAGAGGCGTTTTTGTACGCGGATTCCAGCATGCGTAGCTCTTCCGAATTGTAGAGATTATCCGTCTCCCGCTTGCCACTCGCATTTCCCATCTCAGGACACGAAATTCAGTTGGCTGGTGCTACGACGGCTGGTAAACAAACAATGCAGCTGCAGCTGCGGATGATCGCGGATTTTAATTGAAAATAAAAAACAAACAACACAAGCTGGTGATGGAATGGCAGTCGATTTCCCTGCGATACCCTCGATAGTTTCGGAAACGGGTAAATTTCGCGGTGGTCGTCTGCACTATGAATGGTTATTGTCTGTGCTAAGTCAGGTCGATGTATTTGCTAAACGGTTAATTTTAACCGCTAAGCGAATTCAACGAAGGCCAAGCGAATAAAATAAATTTTAAATGCCTTGGATAGCTGTAGTTAAAAATTTATTAAAATAAAAAAAAACTTACAAGTTTGAGCTAAGATACCTTTGCCCTAGCCCGGTATTTTTGAAATTATCTCAGTTACCTTAGCAACACGCCTTATTAAATCAATTGCCTGGCCTAATAAAGTTCTCCCACACAAAAACACAATAAATTCCGATACAAAACTGAAGAAAGCTGGCATACCGACAGATATTTCCGGAATACGATTGGCCAAATCTGTGAGTAATCCAACGCGAAAGGATGATTGGCACTAGCAGCGGAACGTCCCACAATCGGAGTCGAAAGAAGAAAGAACAGTGCGGATCCTGTCCCAACAGATTCTCAAAGGATAAGCGCCAGGTGGCCGCCGAGGACTCGGATACCACGGAGGTGGAATCGTCTACGGATGAGGAGGAACGC

 

---

Search Again