• About
• Search
• MiMIC
  • Technique
  • Constructs
  • RMCE Lines
• Transposons
  • GDP
  • Others
• CRIMIC
  • Technique
  • Constructs
• Order
• Downloads

CRIMIC CR00740

Gene ssp3

CG18397

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:18938276..18938298	37B6	FBti0200650	80635

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	short band	AACATCGAACACATCCGGCAAAGAGC	ACGGACAGAGCAACAGAGAAAGAGAC

Images

Construct Design Sequences

sgRNA sequence	TCTTTATAGCGGGTTTAAGTTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GAGCGACAATGAACACGAGC	AAGAAATCCTAGTGGGGCGG	AGAGACATAAACGCGGCCAA	TGCGTGTCATACCCAAAGCT

 

Amplicon 5'

Amplicon 3'

GAGCGACAATGAACACGAGCCAAGTTAACACAATGCCAACGACCAAAACAACAACTCCGAAGTGCGATGCGCCGTCAAAATATTTTTATAGATTTAAATAAAAAGCAAGAAATTATGTCCTATCAAAATGGAGAAGAAAACCGATTTGCAATACTTTTTCGTCTTGAGAAAATATATATATAATTATACGTGTTGAACGATATTTCTTTAAGCTTTAAAATTACATCAAATATAAAAAACATAAATTATTGTTTTTACTATCACAAAAATCAAATATTTTTGTTTTTACATATTTTTTTTTAATTTCTGTAAATTTGATATATATGTATATTCTATATATATTTATTTTTTTCTGATCTGACCTGATGTTCTCGCGGAAAAAAGGGTACAATTATATAGGCACCAATAAATTTAAAAAAAGGCAACAAAAATTTACATATATTGGGTGGAAGAGTGGCTTTTTATGCGATTTAACGATTGATTTAACAGCAGCCGCAAATGTACATATAAGCATTTTACACGCCGACAAATTGGACAGAAGAGTGAAGAGCTCCAGAAAGTTGAGATCAATGGGGGGCAGGGATCAGGGAACCGACCTAGTGCTTAAAAATTGATGGGCCAGGCGACAGACAAGCGAGCGCACGAGGCCAGTAAATAAAATTCAAATAAAAATGCCAAACAAACAAAAGGCAGCGCCAAATAAAAGCGTCAAAGAAATGACAAATAAACAACGCCAGCGAAACGAGCAAAAACAAAAGCCAGCAAAACATAAACTTTAAACAGGGCCAAAAGGGTATTGCGGGCTAATCGACCACCAAATGCCCTTCCAAACCAATTGGGAGTTACATAAATTTGCATAACTTGTATAATAAAAAAATGTGGTATATATTAATATATATAATAAATATTTATGGAAATATATAGAACTATTCAAAAAACTAGTGTTAAAATAGTGGCATATCTAAACAATTCTGTAAGGCAATCGAATTGAATAATACAAAATAAATATTAACAACTTTTCTACACTTAGTTCACATAAATGGCCATCTCCACGACTATATAGTTGCTGCAAGACCTTCGCTTAATCCTCCGGACTCCCGCCCCACTAGGATTTCTT

AGAGACATAAACGCGGCCAAGTGTTAAGTGGCTGCCGGCAGGAATAAAACGCTAGCCAAGTACGAATTTATACCACACACATAGGCGACTTTCGACGTGGCCTGGGGAATTGTGAGTTGCCAACTTTAGTTGGGTCTGCCCCAATCCCACGGACAACGCCTCGAGGCGAGACGACTGCCTGGCGACACTTTTATTGTGGAGCATTTTGGGTGGCCAAAAGTTCGAGAAAGTTTCTTTTGTGCAGCGGTTGCTTTTGAGGATTACCTGGAAACTCTTGTTGATGGCATCGAACAGAACGGTTATGGAATTGCCCAGAAGAGCGTGACGAGCAATCTGAGGACACACGGAACAGGCATTCCGGTACAGCTGCGCGGCCAAATAGACGGACCTAGAGCATTATAAAGTAGCGATATTAACGATATACTCAAAAGGTAAAAAAGTCCAAAGTCAACTCACTTCCGGGAGATCTCTGAGTTTTCATCCATGCCCTTGCTAATCACGTGGTTGGCCAGTATGCCCAAGCCATCCATGCAGAGAAAAGCTAGAAAATCTAAGGTCTGAGCTTTGGGTATGACACGCA

 

---

Search Again