• About
• Search
• MiMIC
  • Technique
  • Constructs
  • RMCE Lines
• Transposons
  • GDP
  • Others
• CRIMIC
  • Technique
  • Constructs
• Order
• Downloads

CRIMIC CR00741

Gene Tret1-1

CG30035

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:11672591..11672603	48B7	FBti0199620	79293

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATTGTCCGCTTGTTAAGCTCGTTGGC	GCCCGATCAATCAGAACGATGCCTAT

Images

Construct Design Sequences

sgRNA sequence	TTCGACTCGACTTATATGGTCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GACCCTGCAACTTCTGTCGA	TAAGTCGAGTCGAACTGGCC	TCGCTTTCGACACACACTTT	CTTCAGTTCGGGCTCCACAT

 

Amplicon 5'

Amplicon 3'

GACCCTGCAACTTCTGTCGATGTTTGGCAGTTGCAGTTGCAGTTTGCATTCGAGCTTCGCTGCGCGTTCGCTGAAAACAGCCTGAAACTGCTTTTGAAAATCGGCATAACGCATTTTCTTTGTCTTTGCCGTTCGAAAACAAACTGATAAGCTTGGTCTTGATCAAACACTTGGGAACAGAATCTCCGTCGCTCGCATAAGCAATATCGGTCCGATCAGCACTCGGTGGCTTCGTGTACGTGCCTGAGTCCCATAAAATCACAGCGAAAAACTATTCTCGAATATTGGAAACTAGTGACAGTGCATAATAACAAGTTTCAGTTGAGTTTTGACGAGCGAACGCGCCAAACGTATTCCATTTCCAAGGAGCAAAGTGAAAGGCAGGAAGCTAACGCTGTATTAGCTACATTCGCCGCTTCGTTGCAAGCCAAAAACGATTTCAGTTCGTGATTCAGTTTAAAGATACAGCACCGCGATGAAGATCCTGATGCGAGCGGACACCCATGTGTCCTTCTCCGTGCCCGTCGAGGAGCCCAAGGCCATTTGTACCTTCTCCCAGGTGCCTGAATTATATTAATTAACAAGTGTGAATGCAATAGTCGTATACTATCAAATACCTATTACTCAGCTAGTGGAAATCCCAAAAGCGAAAATTCGTAAATTTGTAGGCTTATCGCTAGATATTCGGGAATAAAATAAGAAAAAATCAAAAAAATTTAAACAGTGTGAACAGTATTGGGCGGTTTGTGGGCGGTGCAACATTTCGAAGTAAACTCGAATCTCAACTCTCTAGTTTTAATAGTTCCGGAGATCGAGGCGTTCATACGGACATGGCCAGTTCGACTCGACTTA

TCGCTTTCGACACACACTTTTCAACGAATCTAGTATACCCTTTTACTCTACGAGTAACTACTGTGGAAAAGTTCGAGTCTTACTAATTAAAATCGTTTTCCTTCCTATTTCCAGGTCTTGGCTGCTCTGAGTGTCTCGCTGGGCTCCCTAGTGGTCGGATTTGTCAGCGCTTACACGTCGCCTGCTCTTGTGTCGATGACCGATCGGAATATCACCTCGTTTGAGGTCACCCAAGATGCTGTAAGTACTATTAAAATGTAATTTGTCGAGGTTAAGTTAACTCTTTGTTCGCATCTCTTGAAATTGTTGGAGATTAGCTAATCCCAAGCTAATAAGTCCCAAAGGAGCGTTTCAAAGTCACACTTTTCCATCGAAACTCGAATTCCCATTCTCAAACGAAGCTAAAACCCTATCTAATCACTTGAAATCTATCATATTGCAGGGTTCTTGGGTTGGTGGCATCATGCCGCTGGCTGGATTGGCAGGTGGCATAGCCGGAGGACCCTTGATCGAATATCTGGGCAGGCGCAACACCATCCTGGCCACTGCGGTGCCCTTCATTGTCTCATCACTACTGATTGCCTGTGCTGTGAATGTGGCTATGGTTCTGTGCGGACGATTCCTGGCTGGATTCTGCGTTGGCATTGCCTCTTTATCCCTGCCCGTCTACCTGGGTGAAACTGTGCAGCCTGAGGTGCGAGGCACTTTGGGTTTGCTGCCCACGGCCTTTGGTAACATAGGCATCCTGCTCTGCTTCGTGGCGGGATCCTTTATGAATTGGTCGATGCTGGCCTTCTTGGGAGCTGCTCTGCCCGTTCCCTTCCTGATTTTAATGTTCCTTATTCCGGAAACTCCGCGCTGGTTTGTGGGCCGTGGTCTGGAGGAGCGCGCCAGGAAGGCTTTGAAATGGCTGCGTGGCAAGGAGGCTGATGTGGAGCCCGAACTGAAG

 

---

Search Again