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CRIMIC CR00742

Gene Ppt2

CG4851

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:11280518..11280553	32E2	FBti0199621	79294

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCAGAGGTGGTCAAGCTGGATTTTGT	ATCTCTGCTGCAATACGCAGGCTGTT

Images

Construct Design Sequences

sgRNA sequence	CACTGTAAACAATAACAAAGGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TTAGGAAAGCCCAAGGAGCG	AACCTCTTCGATTTCCCGGA	GGCAAGGTCGAATTTGACGC	CAATGATAAGCTTGCCGGCC

 

Amplicon 5'

Amplicon 3'

TTAGGAAAGCCCAAGGAGCGTAGAAATTTAGTTATTTGCAATGGCTTCTACAATCTCATTGCTGTAATTTGTACTTCATACTTTTATTTTGATCAGACTGTATTATAAAAGCATATATATAATTATATTTTTAAAGCACCCAATGCAAACTCAATTGTGTTTCTAATGGTCCGCTAAATTATATTGCATTTTATCTTTATTTGAATGTACCCATCCAATTTATGGGACCTATTTTATAAACCTTTCCTTTTAAATGCACACCACGTATTTTAAATTCATCCAATTATTGTACAATGAAGTCTCGAGAAATTTCGAATGCATTTTCAAATTTCGTTCTGCTCATAATTGCGCTCGCAACCCATACATTGCCATTCGCATGTCGGATTTCAAATCCGCACTTTCAGAATTTCCAAAGCGGGTAGCAAGAAGAAGAGCGGCTAATAAAGATACGGATACATCAACAACAACAGTCGGAAATCCATTGTTCCCCACAAAATGAGACTTCGTTTGCAAGTCCTGGTGGCCCTGTTAACCTGCTCGTCCATTTCCGTCTCACTGGCCTACAAACCGGTGGTCATACTCCATGGAATTCTATCAGGAGCCGAATCGATGGCCTCTCTGGTCCGGGAAATCGAAGAGGTT

GGCAAGGTCGAATTTGACGCAAACAACGCAGTAGCACTGATAAACTCATTTCGTCCGTTTGTAGTTTCATCCTGGCACTATCGTCTACAACTGCGACAAGTTCAATGGATGGTACAGCTTGGAGAACGCCTGGCGACAGGTCGATCAGGTTAGGGATTATCTCAACGAGGTGGGCAAACTGCATCCAGAGGGCATCATTGTGCTGGGTAAGGCAGCCGATTTTCATTAGAGTATTTTCTTTTAGTTACCAATACTATGCCTTTTTCAGGATACTCACAAGGAGGCCTTTTGGCCCGGGCGGCCATCCAAAGTCTGCCCGAGCACAATGTGAAGACCTTTATATCTCTATCCTCGCCACAGGCGGGGCAATACGGCAGTAAGTGAGATCAAAGTTTAAACCAATGAGTAAATTCTATTTATATAGATGCATAACTGCGTCACATTTTTCCGGGCTAAACTCTTTTAAAGCTTACAAGAAACAAATATATTATGTAACTGTGTTCAAAAATTACGCAGTTGGGTTATAAAACAAAGGGGGATCACAATGGTTCAAGTTTTTATTTTTTGGTAATATTTTCAGCTAGCTTCTTGCATCTAATCTTTCCCGACTTGGCGGCCAAGACAGCCTTCGAATTGTTCTACTCGCGCGTGGGACAGCACACCTCGGTTGGTGGCTACTGGAACGATCCGCAGAGGCAGGATCTCTACCTGAAGTACAGCGAATTCCTGCCACTGATCAATAATGAGAAGAAGACCTCCAACTCCACATCTTTTAAGATGGGAATGGTGCGTCTAAATAAGTTGGTAATGATCGGCGGACCCAACGACGATGTGATTACTCCGTGGCAATCGAGGTATGGTTTACTTAGTCGGATATTATCAAAGGTGAAATAACCACAAATCGTATATATTTCCTCAGCCACTTTGGTTACTTTGACGAGAATATGGATGTGATTCCATTTATTAGGCGACCGATTTTCACCAGCGACTCCATTGGCATCAGGACGCTTCAGGAGGCCGGCAAGCTTATCATTG

 

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