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CRIMIC CR00745

Gene ave

CG30476

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:14752801..14752824	51C2	FBti0200653	80638

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCGAAGAAATGTGCGCTTCTCCTGTT	GAAGAGACACATGACATGTGGGTAGC

Images

Construct Design Sequences

sgRNA sequence	ACCGTCGAGCCTCGTTGGAACGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ATATGCGAGTCTTAGCGGGC	GGCTCGACGGTAAGTGAAGT	GCAGTTCCGTCATTTCAGCA	CGACGTCCAGTTGATTTCGC

 

Amplicon 5'

Amplicon 3'

ATATGCGAGTCTTAGCGGGCATCGATAGGCCACGAATGTCGTACTAGTGCTTGTTGTCGATATATCGCACACAACTCGTGAATTTCTTAGGATATCGTTTATACCTTTGATTTAAATCAGTCGGATTAGGGAACTTATCGGCCGATTGTGTCACCCCTAATTTACATTTTTTTATATTTACATTACAGTAATACAACTGTAATCGATATCTTGTCTCACACCTAATTAATATCGCCTAGCTACCATCACTATTTGTTTTGTTGCTATTTGCAAATAAATCTTAAATTTCTCGATTTGTTTTTTGTGAAATGGGTGAAGAAACTATTAACTCAACGCAAAACAAAACCAGAACGAAAACTACGCGACCGAAGGCAGTGTACCTGTGGACAGTTAGCGATGTGCTCAAGTGGTATCGCCGCCACTGCGGTGAATACACCCAGTATGAGCAGCTCTTCGCCCAGGTGAGTTGAGCCCACAATCCAAATCGTAGTCCTTGATCTACTTCACTTACCGTCGAGCC

GCAGTTCCGTCATTTCAGCACCTCAAAACACAAACAGGAAAATTCCCGGTTACCTTTTGCTAAAATTTAGACATCACTATAACTGCCTACTGATCTTGTTTAATTTCTTTCCATGTACACAGCACGATATAACCGGAAGGGCTCTACTCCGGATCACAGACTCCTCACTGCAAAGAATGGGCGTGACGGACAACCGGGATCGGGAAGCCATTTGGCGGGAGATCGTTAAGCAGCGACTGAAGACGGATATCATGGAAATCCGGGATATGGAAAGGCTCAATATTTACTAGGGTCACGATATACTCGTTTTATTTGCTGTAATAGGAGAATAGTTAATGCGTATTTTGTAAACTTTCTTTTTAAGATTCTTAGACGCCATATCTGGTTTTAGATTAGACAATCATGTATGCTATTAATCAAATATATAAGAGCGCAAAATTCTAGATTAAATTTAAATATTGACTAATTATTGCTTATTGCCCATCATTTTAAATATGAAAATAACCCTAGAATGAGAGATTTCTTTGGTTTTTATTATTAGGATACTGGTTTTAGAGTCTACTAAAGTAAAGACCAGAATATCATTTCTTTGAGATCACTTAAGTGTGTTTCTAATATCAATCACTTATAACGACTAGTTCCTCTTAATCAGATCTCCTTTTCCAGCTTGGGCGAAATCAACTGGACGTCG

 

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