CR00745

CRIMIC CR00745

Gene ave

CG30476

Information

Donor Location Cytology FBti Stock number
pM37-p02R:14752801..1475282451C2 FBti020065380638

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK GCGAAGAAATGTGCGCTTCTCCTGTT GAAGAGACACATGACATGTGGGTAGC

Images

CR00745-1.jpgCR00745-4-1.jpgCR00745-4-2.jpgCR00745-4-3.jpg

Construct Design Sequences

sgRNA sequence ACCGTCGAGCCTCGTTGGAACGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
ATATGCGAGTCTTAGCGGGC GGCTCGACGGTAAGTGAAGT GCAGTTCCGTCATTTCAGCA CGACGTCCAGTTGATTTCGC
 
Amplicon 5' Amplicon 3'
ATATGCGAGTCTTAGCGGGCATCGATAGGCCACGAATGTCGTACTAGTGCTTGTTGTCGATATATCGCACACAACTCGTGAATTTCTTAGGATATCGTTTATACCTTTGATTTAAATCAGTCGGATTAGGGAACTTATCGGCCGATTGTGTCACCCCTAATTTACATTTTTTTATATTTACATTACAGTAATACAACTGTAATCGATATCTTGTCTCACACCTAATTAATATCGCCTAGCTACCATCACTATTTGTTTTGTTGCTATTTGCAAATAAATCTTAAATTTCTCGATTTGTTTTTTGTGAAATGGGTGAAGAAACTATTAACTCAACGCAAAACAAAACCAGAACGAAAACTACGCGACCGAAGGCAGTGTACCTGTGGACAGTTAGCGATGTGCTCAAGTGGTATCGCCGCCACTGCGGTGAATACACCCAGTATGAGCAGCTCTTCGCCCAGGTGAGTTGAGCCCACAATCCAAATCGTAGTCCTTGATCTACTTCACTTACCGTCGAGCC GCAGTTCCGTCATTTCAGCACCTCAAAACACAAACAGGAAAATTCCCGGTTACCTTTTGCTAAAATTTAGACATCACTATAACTGCCTACTGATCTTGTTTAATTTCTTTCCATGTACACAGCACGATATAACCGGAAGGGCTCTACTCCGGATCACAGACTCCTCACTGCAAAGAATGGGCGTGACGGACAACCGGGATCGGGAAGCCATTTGGCGGGAGATCGTTAAGCAGCGACTGAAGACGGATATCATGGAAATCCGGGATATGGAAAGGCTCAATATTTACTAGGGTCACGATATACTCGTTTTATTTGCTGTAATAGGAGAATAGTTAATGCGTATTTTGTAAACTTTCTTTTTAAGATTCTTAGACGCCATATCTGGTTTTAGATTAGACAATCATGTATGCTATTAATCAAATATATAAGAGCGCAAAATTCTAGATTAAATTTAAATATTGACTAATTATTGCTTATTGCCCATCATTTTAAATATGAAAATAACCCTAGAATGAGAGATTTCTTTGGTTTTTATTATTAGGATACTGGTTTTAGAGTCTACTAAAGTAAAGACCAGAATATCATTTCTTTGAGATCACTTAAGTGTGTTTCTAATATCAATCACTTATAACGACTAGTTCCTCTTAATCAGATCTCCTTTTCCAGCTTGGGCGAAATCAACTGGACGTCG
 
Search Again